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<title lang=en>Localization and in situ absolute quantification of chlordecone in the mouse liver by MALDI imaging.</title>
<creator>Lagarrigue, Mélanie</creator>
<creator>Lavigne, Régis</creator>
<creator>Tabet, Elise</creator>
<creator>Genet, Valentine</creator>
<creator>Thomé, Jean-Pierre</creator>
<creator>Rondel, Karine</creator>
<creator>Guével, Blandine</creator>
<creator>Multigner, Luc</creator>
<creator>Samson, Michel</creator>
<creator>Pineau, Charles</creator>
<contributor>Plateforme Protéomique-Biogenouest (PPB) ; Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) - Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) - Proteomics Core Facility (Protim) ; Université de Rennes 1 (UR1) - Plateforme Génomique Santé Biogenouest® - Plateforme Génomique Santé Biogenouest®</contributor>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Center for Analytical Research and Technology ; Université de Liège</contributor>
<description>International audience</description>
<source>ISSN: 0003-2700</source>
<source>EISSN: 1520-6882</source>
<source>Analytical Chemistry</source>
<publisher>American Chemical Society</publisher>
<identifier>hal-01118103</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01118103</identifier>
<source>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01118103</source>
<source>Analytical Chemistry, American Chemical Society, 2014, 86 (12), pp.5775-83. 〈10.1021/ac500313s〉</source>
<identifier>PUBMED : 24837422</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/24837422</relation>
<identifier>DOI : 10.1021/ac500313s</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1021/ac500313s</relation>
<language>en</language>
<subject>[CHIM] Chemical Sciences</subject>
<subject>[SDV] Life Sciences [q-bio]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Chlordecone is an organochlorine pesticide that was extensively used in the French West Indies to fight weevils in banana plantations from 1973 to 1993. This has led to a persistent pollution of the environment and to the contamination of the local population for several decades with effects demonstrated on human health. Chlordecone accumulates mainly in the liver where it is known to potentiate the action of hepatotoxic agents. However, there is currently no information on its in situ localization in the liver. We have thus evaluated a matrix-assisted laser desorption ionization (MALDI) imaging quantification method based on labeled normalization for the in situ localization and quantification of chlordecone. After validating the linearity and the reproducibility of this method, quantitative MALDI imaging was used to study the accumulation of chlordecone in the mouse liver. Our results revealed that normalized intensities measured by MALDI imaging could be first converted in quantitative units. These quantities appeared to be different from absolute quantities of chlordecone determined by gas chromatography (GC), but they were perfectly correlated (R(2) = 0.995). The equation of the corresponding correlation curve was thus efficiently used to convert quantities measured by MALDI imaging into absolute quantities. Our method combining labeled normalization and calibration with an orthogonal technique allowed the in situ absolute quantification of chlordecone by MALDI imaging. Finally, our results obtained on the pathological mouse liver illustrate the advantages of quantitative MALDI imaging which preserves information on in situ localization without radioactive labeling and with a simple sample preparation.</description>
<date>2014-06-17</date>
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