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<datestamp>2018-01-11</datestamp>
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<title lang=en>Looking for Missing Proteins in the Proteome of Human Spermatozoa: An Update</title>
<creator>Vandenbrouck, Y.</creator>
<creator>Lane, L.</creator>
<creator>Carapito, C.</creator>
<creator>Duek, P.</creator>
<creator>Rondel, Karine</creator>
<creator>Bruley, C.</creator>
<creator>Macron, C.</creator>
<creator>Gonzalez De Peredo, A.</creator>
<creator>Couté, Y.</creator>
<creator>Chaoui, K.</creator>
<creator>Com, Emmanuelle</creator>
<creator>Gateau, A.</creator>
<creator>Hesse, A.-M.</creator>
<creator>Marcellin, M.</creator>
<creator>Méar, Loren</creator>
<creator>Mouton-Barbosa, E.</creator>
<creator>Robin, T.</creator>
<creator>Burlet-Schiltz, O.</creator>
<creator>Cianferani, S.</creator>
<creator>Ferro, M.</creator>
<creator>Freour, T.</creator>
<creator>Lindskog, C.</creator>
<creator>Garin, J.</creator>
<creator>Pineau, C.</creator>
<contributor>Laboratoire de Spectrométrie de masse BioOrganique (CNRS UMR 7178) ; UdS</contributor>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Institut de pharmacologie et de biologie structurale (IPBS) ; Université Paul Sabatier - Toulouse 3 (UPS) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Centre de génétique moléculaire et cellulaire (CGMC) ; Université Claude Bernard Lyon 1 (UCBL) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>IxFiber SAS (IxFiber) ; industriel</contributor>
<contributor>Médecine et Biologie du Développement et de la Reproduction ; CHU Nantes</contributor>
<contributor>Université Joseph Fourier - Grenoble 1 (UJF)</contributor>
<description>International audience</description>
<source>Journal of Proteome Research</source>
<identifier>hal-01406074</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01406074</identifier>
<source>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01406074</source>
<source>Journal of Proteome Research, 2016, 15, pp.3998--4019. 〈10.1021/acs.jproteome.6b00400〉</source>
<identifier>DOI : 10.1021/acs.jproteome.6b00400</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.jproteome.6b00400</relation>
<identifier>PUBMED : 27444420</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/27444420</relation>
<language>en</language>
<subject>[SDV.EE.SANT] Life Sciences [q-bio]/Ecology, environment/Health</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>The Chromosome-Centric Human Proteome Project (C-HPP) aims to identify "missing" proteins in the neXtProt knowledgebase. We present an in-depth proteomics analysis of the human sperm proteome to identify testis-enriched missing proteins. Using protein extraction procedures and LC-MS/MS analysis, we detected 235 proteins (PE2-PE4) for which no previous evidence of protein expression was annotated. Through LC-MS/MS and LC-PRM analysis, data mining, and immunohistochemistry, we confirmed the expression of 206 missing proteins (PE2-PE4) in line with current HPP guidelines (version 2.0). Parallel reaction monitoring acquisition and sythetic heavy labeled peptides targeted 36 ≪one-hit wonder≫ candidates selected based on prior peptide spectrum match assessment. 24 were validated with additional predicted and specifically targeted peptides. Evidence was found for 16 more missing proteins using immunohistochemistry on human testis sections. The expression pattern for some of these proteins was specific to the testis, and they could possibly be valuable markers with fertility assessment applications. Strong evidence was also found of four "uncertain" proteins (PE5); their status should be re-examined. We show how using a range of sample preparation techniques combined with MS-based analysis, expert knowledge, and complementary antibody-based techniques can produce data of interest to the community. All MS/MS data are available via ProteomeXchange under identifier PXD003947. In addition to contributing to the C-HPP, we hope these data will stimulate continued exploration of the sperm proteome. © 2016 American Chemical Society.</description>
<date>2016</date>
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