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<title lang=en>A misleading false-negative result of Pneumocystis real-time PCR assay due to a rare punctual mutation: A French multicenter study.</title>
<creator>Le Gal, Solène</creator>
<creator>Robert-Gangneux, Florence</creator>
<creator>Pepino, Yann</creator>
<creator>Belaz, Sorya</creator>
<creator>Damiani, Céline</creator>
<creator>Guéguen, Paul</creator>
<creator>Pitous, Melanie</creator>
<creator>Virmaux, Michèle</creator>
<creator>Lissillour, Eloise</creator>
<creator>Pougnet, Laurence</creator>
<creator>Guillaud-Saumur, Thibaud</creator>
<creator>Toubas, Dominique</creator>
<creator>Valot, Stephane</creator>
<creator>Hennequin, Christophe</creator>
<creator>Morio, Florent</creator>
<creator>Hasseine, Lilia</creator>
<creator>Bouchara, Jean-Philippe</creator>
<creator>Totet, Anne</creator>
<creator>Nevez, Gilles</creator>
<contributor>Laboratoire Universitaire de Biodiversité et Ecologie Microbienne (LUBEM) ; Université européenne de Bretagne (UEB) - Université de Brest (UBO) - IFR148 ScInBioS</contributor>
<contributor>Laboratoire de Parasitologie-Mycologie ; Centre Hospitalier Régional Universitaire de Brest (CHRU Brest)</contributor>
<contributor>Service de Parasitologie-Mycologie [Rennes] ; Université de Rennes 1 (UR1) - Hôpital Pontchaillou - CHU Pontchaillou [Rennes]</contributor>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Université de Brest (UBO)</contributor>
<contributor>UMR-I 01 - AMIENS ; Université de Picardie Jules Verne (UPJV)</contributor>
<contributor>Laboratoire de Parasitologie et Mycologie (Parasito - Myco - AMIENS) ; CHU Amiens-Picardie</contributor>
<contributor>Génétique, génomique fonctionnelle et biotechnologies ; EFS - Université de Brest (UBO) - Institut National de la Santé et de la Recherche Médicale (INSERM)</contributor>
<contributor>Laboratoire de Génétique Moléculaire et d'Histocompatibilité [Brest] ; Centre Hospitalier Régional Universitaire de Brest (CHRU Brest) - Hôpital Morvan [Brest]</contributor>
<contributor>Matrice extracellulaire et dynamique cellulaire - UMR 7369 (MEDyC) ; Université de Reims Champagne-Ardenne (URCA) - SFR CAP Santé (Champagne-Ardenne Picardie Santé) ; Université de Reims Champagne-Ardenne (URCA) - Université de Picardie Jules Verne (UPJV) - Université de Reims Champagne-Ardenne (URCA) - Université de Picardie Jules Verne (UPJV) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>CHU Reims</contributor>
<contributor>CHU Dijon ; Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon)</contributor>
<contributor>Service de Parasitologie - Mycologie [CHU Saint-Antoine] ; Assistance publique - Hôpitaux de Paris (AP-HP) - CHU Saint-Antoine [APHP]</contributor>
<contributor>Centre d'Immunologie et de Maladies Infectieuses (CIMI) ; Université Pierre et Marie Curie - Paris 6 (UPMC) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>CHU Nantes</contributor>
<contributor>CHU Nice</contributor>
<contributor>Groupe d'Etude des Interactions Hôte-Parasite (GEIHP) ; Université d'Angers (UA)</contributor>
<contributor>Departments of Medical Parasitology and Mycology ; Departments of Medical Parasitology and Mycology</contributor>
<description>International audience</description>
<source>Medical Mycology</source>
<identifier>hal-01477200</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01477200</identifier>
<source>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01477200</source>
<source>Medical Mycology, 2017, 55, pp.180-184. 〈10.1093/mmy/myw051〉</source>
<identifier>DOI : 10.1093/mmy/myw051</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1093/mmy/myw051</relation>
<identifier>PUBMED : 27489302</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/27489302</relation>
<language>en</language>
<subject>[SDV.EE] Life Sciences [q-bio]/Ecology, environment</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>This article describes a previously unreported mutation at position 210 (C210T) of the mitochondrial large subunit ribosomal RNA (mtLSUrRNA) gene of Pneumocystis jirovecii, which led to a false-negative result of a real-time polymerase chain reaction (PCR) assay. Since the aforementioned real-time PCR assay is widely used in France, a French multicenter study was conducted to estimate the mutation frequency and its potential impact on the routine diagnosis of Pneumocystis pneumonia (PCP). Through analysis of data obtained from eight centers, the mutation frequency was estimated at 0.28%. This low frequency should not call into question the routine use of this PCR assay. Nonetheless, the occurrence of the false-negative PCR result provides arguments for maintaining microscopic techniques combined to PCR assays to achieve PCP diagnosis.</description>
<date>2017</date>
</dc>
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