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<title lang=en>New analysis workflow for MALDI imaging mass spectrometry: application to the discovery and identification of potential markers of childhood absence epilepsy.</title>
<creator>Lagarrigue, Mélanie</creator>
<creator>Alexandrov, Theodore</creator>
<creator>Dieuset, Gabriel</creator>
<creator>Perrin, Aline</creator>
<creator>Lavigne, Régis</creator>
<creator>Baulac, Stéphanie</creator>
<creator>Thiele, Herbert</creator>
<creator>Martin, Benoit</creator>
<creator>Pineau, Charles</creator>
<contributor>Plateforme Protéomique-Biogenouest (PPB) ; Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) - Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) - Proteomics Core Facility (Protim) ; Université de Rennes 1 (UR1) - Plateforme Génomique Santé Biogenouest® - Plateforme Génomique Santé Biogenouest®</contributor>
<contributor>Center of Industrial Mathematics - University of Bremen ; University of Bremen</contributor>
<contributor>Laboratoire Traitement du Signal et de l'Image (LTSI) ; Université de Rennes 1 (UR1) - Institut National de la Santé et de la Recherche Médicale (INSERM)</contributor>
<contributor>Centre de Recherche de l'Institut du Cerveau et de la Moelle épinière (CRICM) ; Centre National de la Recherche Scientifique (CNRS) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Université Pierre et Marie Curie - Paris 6 (UPMC)</contributor>
<description>International audience</description>
<source>ISSN: 1535-3893</source>
<source>EISSN: 1535-3907</source>
<source>Journal of Proteome Research</source>
<publisher>American Chemical Society</publisher>
<identifier>hal-00877728</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-00877728</identifier>
<source>https://hal.archives-ouvertes.fr/hal-00877728</source>
<source>Journal of Proteome Research, American Chemical Society, 2012, 11 (11), pp.5453-63. 〈10.1021/pr3006974〉</source>
<identifier>DOI : 10.1021/pr3006974</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1021/pr3006974</relation>
<identifier>PUBMED : 22994238</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/22994238</relation>
<language>en</language>
<subject lang=en>imaging mass spectrometry</subject>
<subject lang=en>absence epilepsy</subject>
<subject lang=en>MALDI-time-of-flight mass spectrometry</subject>
<subject lang=en>protein markers</subject>
<subject lang=en>classification method</subject>
<subject>[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Childhood absence epilepsy is a prototypic form of generalized nonconvulsive epilepsy characterized by short impairments of consciousness concomitant with synchronous and bilateral spike-and-wave discharges in the electroencephalogram. For scientists in this field, the BS/Orl and BR/Orl mouse lines, derived from a genetic selection, constitute an original mouse model "in mirror" of absence epilepsy. The potential of MALDI imaging mass spectrometry (IMS) for the discovery of potential biomarkers is increasingly recognized. Interestingly, statistical analysis tools specifically adapted to IMS data sets and methods for the identification of detected proteins play an essential role. In this study, a new cross-classification comparative design using a combined discrete wavelet transformation-support vector machine classification was developed to discriminate spectra of brain sections of BS/Orl and BR/Orl mice. Nineteen m/z ratios were thus highlighted as potential markers with very high recognition rates (87-99%). Seven of these potential markers were identified using a top-down approach, in particular a fragment of Synapsin-I. This protein is yet suspected to be involved in epilepsy. Immunohistochemistry and Western Blot experiments confirmed the differential expression of Synapsin-I observed by IMS, thus tending to validate our approach. Functional assays are being performed to confirm the involvement of Synapsin-I in the mechanisms underlying childhood absence epilepsy.</description>
<date>2012-11-02</date>
</dc>
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