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<title lang=en>Proteomics and integrative genomics for unraveling the mysteries of spermatogenesis: the strategies of a team</title>
<creator>Com, Emmanuelle</creator>
<creator>Melaine, Nathalie</creator>
<creator>Chalmel, Frédéric</creator>
<creator>Pineau, Charles</creator>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<description>International audience</description>
<source>ISSN: 1874-3919</source>
<source>EISSN: 1876-7737</source>
<source>Journal of Proteomics</source>
<publisher>Elsevier</publisher>
<identifier>hal-01063931</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01063931</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01063931</source>
<source>Journal of Proteomics, Elsevier, 2014, 107, pp.128--143. 〈10.1016/j.jprot.2014.04.013〉</source>
<identifier>DOI : 10.1016/j.jprot.2014.04.013</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jprot.2014.04.013</relation>
<identifier>PUBMED : 24751586</identifier>
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<language>en</language>
<subject>[SDV] Life Sciences [q-bio]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>The strikingly complex structural organization of the mammalian testis in vivo creates particular difficulties for studies of its organization, function and regulation. These difficulties are particularly pronounced for investigations of the molecular communication networks within the seminiferous tubules that govern spermatogenesis. The use of classical molecular and cell biology approaches to unravel this complexity has proved problematic, due to difficulties in maintaining differentiated germ cells in vitro, in particular. The lack of a suitable testing ground has led to a greater reliance on high-quality proteomic and genomic analyses as a prelude to the in vitro antx1d in vivo testing of hypotheses. In this study, we highlight the options currently available for research, as used in our laboratory, in which proteomic and integrative genomic strategies are applied to the study of spermatogenesis in mammals. We will comment on results providing insight into the molecular mechanisms underlying normal and pathological spermatogenesis and new perspectives for the treatment of male infertility in humans. Finally, we will discuss the relevance of our strategies and the unexpected potential and perspectives they offer to teams involved in the study of male reproduction, within the framework of the Human Proteome Project. SIGNIFICANCE: Integrative genomics is becoming a powerful strategy for discovering the biological significance hidden in proteomic datasets. This work introduces some of the integrative genomic concepts and works used by our team to gain new insight into mammalian spermatogenesis, a remarkably sophisticated process. We demonstrate the relevance of these integrative approaches to understand the cellular cross talks established between the somatic Sertoli cells and the germ cell lineage, within the seminiferous epithelium. Our work also contributes to new knowledge on the pathophysiology of testicular function, with promising clinical applications. This article is part of a Special Issue entitled: 20years of Proteomics in memory of Viatliano Pallini. Guest Editors: Luca Bini, Juan J. Calvete, Natacha Turck, Denis Hochstrasser and Jean-Charles Sanchez.</description>
<date>2014</date>
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