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<datestamp>2017-12-21</datestamp>
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<title lang=en>Research resource: the dynamic transcriptional profile of sertoli cells during the progression of spermatogenesis</title>
<creator>Zimmermann, Céline</creator>
<creator>Stévant, Isabelle</creator>
<creator>Borel, Christelle</creator>
<creator>Conne, Béatrice</creator>
<creator>Pitetti, Jean-Luc</creator>
<creator>Calvel, Pierre</creator>
<creator>Kaessmann, Henrik</creator>
<creator>Jégou, Bernard</creator>
<creator>Chalmel, Frédéric</creator>
<creator>Nef, Serge</creator>
<contributor>Department of Genetic Medicine and Development ; University of Geneva Medical School</contributor>
<contributor>Université de Genève (UNIGE)</contributor>
<contributor>Ecole Médicale, Département de Médecine Génétique et Développement ; Université de Genève (UNIGE)</contributor>
<contributor>Center for Integrative Genomics - Institute of Bioinformatics, Génopode (CIG) ; University of Lausanne</contributor>
<contributor>École des Hautes Études en Santé Publique [EHESP] (EHESP)</contributor>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Virus, Environnement et Reproduction ; Groupe d'Etude de la Reproduction Chez l'Homme et les Mammiferes (GERHM) ; Université de Rennes 1 (UR1) - IFR140 - Institut National de la Santé et de la Recherche Médicale (INSERM) - Université de Rennes 1 (UR1) - IFR140 - Institut National de la Santé et de la Recherche Médicale (INSERM) - Department of Genetic Medicine and Development ; University of Geneva Medical School - University of Geneva Medical School</contributor>
<description>International audience</description>
<source>ISSN: 0888-8809</source>
<source>Molecular Endocrinology -Baltimore-</source>
<publisher>Endocrine Society</publisher>
<identifier>hal-01146846</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01146846</identifier>
<source>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01146846</source>
<source>Molecular Endocrinology -Baltimore-, Endocrine Society, 2015, 29 (4), pp.627--642. 〈10.1210/me.2014-1356〉</source>
<identifier>DOI : 10.1210/me.2014-1356</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1210/me.2014-1356</relation>
<identifier>PUBMED : 25710594</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/25710594</relation>
<language>en</language>
<subject>[SDV] Life Sciences [q-bio]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Sertoli cells (SCs), the only somatic cells within seminiferous tubules, associate intimately with developing germ cells. They not only provide physical and nutritional support but also secrete factors essential to the complex developmental processes of germ cell proliferation and differentiation. The SC transcriptome must therefore adapt rapidly during the different stages of spermatogenesis. We report comprehensive genome-wide expression profiles of pure populations of SCs isolated at 5 distinct stages of the first wave of mouse spermatogenesis, using RNA sequencing technology. We were able to reconstruct about 13 901 high-confidence, nonredundant coding and noncoding transcripts, characterized by complex alternative splicing patterns with more than 45% comprising novel isoforms of known genes. Interestingly, roughly one-fifth (2939) of these genes exhibited a dynamic expression profile reflecting the evolving role of SCs during the progression of spermatogenesis, with stage-specific expression of genes involved in biological processes such as cell cycle regulation, metabolism and energy production, retinoic acid synthesis, and blood-testis barrier biogenesis. Finally, regulatory network analysis identified the transcription factors endothelial PAS domain-containing protein 1 (EPAS1/Hif2α), aryl hydrocarbon receptor nuclear translocator (ARNT/Hif1β), and signal transducer and activator of transcription 1 (STAT1) as potential master regulators driving the SC transcriptional program. Our results highlight the plastic transcriptional landscape of SCs during the progression of spermatogenesis and provide valuable resources to better understand SC function and spermatogenesis and its related disorders, such as male infertility.</description>
<date>2015</date>
</dc>
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