untitled
<OAI-PMH schemaLocation=http://www.openarchives.org/OAI/2.0/ http://www.openarchives.org/OAI/2.0/OAI-PMH.xsd>
<responseDate>2018-01-15T18:40:53Z</responseDate>
<request identifier=oai:HAL:hal-00681969v1 verb=GetRecord metadataPrefix=oai_dc>http://api.archives-ouvertes.fr/oai/hal/</request>
<GetRecord>
<record>
<header>
<identifier>oai:HAL:hal-00681969v1</identifier>
<datestamp>2017-12-21</datestamp>
<setSpec>type:ART</setSpec>
<setSpec>subject:sdv</setSpec>
<setSpec>collection:UNIV-GRENOBLE1</setSpec>
<setSpec>collection:IFR140</setSpec>
<setSpec>collection:IRSET</setSpec>
<setSpec>collection:UNIV-RENNES1</setSpec>
<setSpec>collection:UNIV-AG</setSpec>
<setSpec>collection:IRTSV-BCI</setSpec>
<setSpec>collection:CEA</setSpec>
<setSpec>collection:UGA</setSpec>
<setSpec>collection:IRSET-HIAEC</setSpec>
<setSpec>collection:BIOSIT</setSpec>
<setSpec>collection:UR1-UFR-SVE</setSpec>
<setSpec>collection:UR1-SDV</setSpec>
<setSpec>collection:UR1-HAL</setSpec>
<setSpec>collection:EHESP</setSpec>
<setSpec>collection:STATS-UR1</setSpec>
<setSpec>collection:USPC</setSpec>
<setSpec>collection:IRSET-2</setSpec>
<setSpec>collection:UNIV-ANGERS</setSpec>
<setSpec>collection:IRSET-EHESP</setSpec>
</header>
<metadata><dc>
<publisher>HAL CCSD</publisher>
<title lang=en>BMP9 is produced by hepatocytes and circulates mainly in an active mature form complexed to its prodomain.</title>
<creator>Bidart, Marie</creator>
<creator>Ricard, Nicolas</creator>
<creator>Levet, Sandrine</creator>
<creator>Samson, Michel</creator>
<creator>Mallet, Christine</creator>
<creator>David, Laurent</creator>
<creator>Subileau, Mariela</creator>
<creator>Tillet, Emmanuelle</creator>
<creator>Feige, Jean-Jacques</creator>
<creator>Bailly, Sabine</creator>
<contributor>Biologie du Cancer et de l'Infection (BCI - UMR S1036) ; Commissariat à l'énergie atomique et aux énergies alternatives (CEA) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Université Grenoble Alpes (UGA)</contributor>
<contributor>Laboratoire de développement et vieillissement de l'endothélium ; Université Joseph Fourier - Grenoble 1 (UJF) - Commissariat à l'énergie atomique et aux énergies alternatives (CEA) - Institut National de la Santé et de la Recherche Médicale (INSERM)</contributor>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<description>International audience</description>
<source>ISSN: 1420-682X</source>
<source>EISSN: 1420-9071</source>
<source>Cellular and Molecular Life Sciences</source>
<publisher>Springer Verlag</publisher>
<identifier>hal-00681969</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-00681969</identifier>
<source>https://hal.archives-ouvertes.fr/hal-00681969</source>
<source>Cellular and Molecular Life Sciences, Springer Verlag, 2012, 69 (2), pp.313-24. 〈10.1007/s00018-011-0751-1〉</source>
<identifier>DOI : 10.1007/s00018-011-0751-1</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1007/s00018-011-0751-1</relation>
<identifier>PUBMED : 21710321</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/21710321</relation>
<language>en</language>
<subject lang=en>BMP9</subject>
<subject lang=en>Liver</subject>
<subject lang=en>Blood</subject>
<subject lang=en>Hepatocytes</subject>
<subject lang=en>ALK1</subject>
<subject lang=en>Endothelial cells</subject>
<subject>[SDV.BC] Life Sciences [q-bio]/Cellular Biology</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Bone Morphogenetic Protein 9 (BMP9) has been recently found to be the physiological ligand for the activin receptor-like kinase 1 (ALK1), and to be a major circulating vascular quiescence factor. Moreover, a soluble chimeric ALK1 protein (ALK1-Fc) has recently been developed and showed powerful anti-tumor growth and anti-angiogenic effects. However, not much is known concerning BMP9. This prompted us to investigate the human endogenous sources of this cytokine and to further characterize its circulating form(s) and its function. Analysis of BMP9 expression reveals that BMP9 is produced by hepatocytes and intrahepatic biliary epithelial cells. Gel filtration analysis combined with ELISA and biological assays demonstrate that BMP9 circulates in plasma (1) as an unprocessed inactive form that can be further activated by furin a serine endoprotease, and (2) as a mature and fully active form (composed of the mature form associated with its prodomain). Analysis of BMP9 circulating levels during mouse development demonstrates that BMP9 peaks during the first 3 weeks after birth and then decreases to 2 ng/mL in adulthood. We also show that circulating BMP9 physiologically induces a constitutive Smad1/5/8 phosphorylation in endothelial cells. Taken together, our results argue for the role of BMP9 as a hepatocyte-derived factor, circulating in inactive (40%) and active (60%) forms, the latter constantly activating endothelial cells to maintain them in a resting state.</description>
<date>2012-01</date>
</dc>
</metadata>
</record>
</GetRecord>
</OAI-PMH>
