untitled
<OAI-PMH schemaLocation=http://www.openarchives.org/OAI/2.0/ http://www.openarchives.org/OAI/2.0/OAI-PMH.xsd>
<responseDate>2018-01-17T12:16:40Z</responseDate>
<request identifier=oai:HAL:hal-01681656v1 verb=GetRecord metadataPrefix=oai_dc>http://api.archives-ouvertes.fr/oai/hal/</request>
<GetRecord>
<record>
<header>
<identifier>oai:HAL:hal-01681656v1</identifier>
<datestamp>2018-01-13</datestamp>
<setSpec>type:ART</setSpec>
<setSpec>subject:sdv</setSpec>
<setSpec>collection:UNIV-RENNES1</setSpec>
<setSpec>collection:BIOSIT</setSpec>
<setSpec>collection:CNRS</setSpec>
<setSpec>collection:UNIV-AG</setSpec>
<setSpec>collection:UNIV-ANGERS</setSpec>
<setSpec>collection:IRSET</setSpec>
<setSpec>collection:IRTSV-BGE</setSpec>
<setSpec>collection:DRS-IPHC</setSpec>
<setSpec>collection:IPHC</setSpec>
<setSpec>collection:UGA</setSpec>
<setSpec>collection:UNIV-NANTES</setSpec>
<setSpec>collection:IRSET-PPB</setSpec>
<setSpec>collection:IFR140</setSpec>
<setSpec>collection:CEA</setSpec>
<setSpec>collection:UR1-HAL</setSpec>
<setSpec>collection:UR1-SDV</setSpec>
<setSpec>collection:STATS-UR1</setSpec>
<setSpec>collection:UR1-UFR-SVE</setSpec>
<setSpec>collection:IRSET-7</setSpec>
<setSpec>collection:EHESP</setSpec>
<setSpec>collection:USPC</setSpec>
</header>
<metadata><dc>
<publisher>HAL CCSD</publisher>
<title lang=en>Validating Missing Proteins in Human Sperm Cells by Targeted Mass-Spectrometry- and Antibody-based Methods</title>
<creator>CARAPITO, Christine</creator>
<creator>Duek, Paula</creator>
<creator>Macron, Charlotte</creator>
<creator>Seffals, Marine</creator>
<creator>Rondel, Karine</creator>
<creator>Delalande, François</creator>
<creator>Lindskog, Cecilia</creator>
<creator>Freour, Thomas</creator>
<creator>Vandenbrouck, Yves</creator>
<creator>Lane, Lydie</creator>
<creator>Pineau, Charles</creator>
<contributor>Institut Pluridisciplinaire Hubert Curien (IPHC) ; Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Swiss Institute of Bioinformatics [Genève] (SIB)</contributor>
<contributor>H2P2 - Histo Pathologie Hight Precision (H2P2) ; Université de Rennes 1 (UR1) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Uppsala University</contributor>
<contributor>CHU Nantes</contributor>
<contributor>Centre de Recherche en Transplantation et Immunologie (CRTI) ; Université de Nantes (UN) - Institut National de la Santé et de la Recherche Médicale (INSERM)</contributor>
<contributor>Commissariat à l'énergie atomique et aux énergies alternatives (CEA)</contributor>
<contributor>Laboratoire de Biologie à Grande Échelle (BGE - UMR S1038) ; Institut National de la Santé et de la Recherche Médicale (INSERM) - Université Grenoble Alpes [Saint Martin d'Hères] - Commissariat à l'énergie atomique et aux énergies alternatives (CEA)</contributor>
<contributor>Université de Grenoble-Alpes</contributor>
<contributor>Swiss Institute of Bioinformatics (SIB) ; Swiss Institute of Bioinformatics (SIB)</contributor>
<contributor>French National Agency for Research (ANR) [ANR-10-INBS-08]</contributor>
<contributor> Biogenouest</contributor>
<contributor> Infrastructures en Biologie Sante et Agronomie (IBiSA)</contributor>
<contributor> Conseil Regional de Bretagne</contributor>
<description>International audience</description>
<source>ISSN: 1535-3893</source>
<source>EISSN: 1535-3907</source>
<source>Journal of Proteome Research</source>
<publisher>American Chemical Society</publisher>
<identifier>hal-01681656</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01681656</identifier>
<source>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01681656</source>
<source>Journal of Proteome Research, American Chemical Society, 2017, 16 (12), pp.4340-4351. 〈10.1021/acs.jproteome.7b00374〉</source>
<identifier>DOI : 10.1021/acs.jproteome.7b00374</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.jproteome.7b00374</relation>
<language>en</language>
<subject lang=en>human proteome project</subject>
<subject lang=en>spermatozoon</subject>
<subject lang=en>missing proteins</subject>
<subject lang=en>parallel reaction monitoring</subject>
<subject lang=en>targeted proteomics</subject>
<subject lang=en>immunohistochemistry</subject>
<subject lang=en>immunocytochemistry</subject>
<subject lang=en>bioinformatics</subject>
<subject lang=en>data mining</subject>
<subject>[SDV] Life Sciences [q-bio]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>The present study is a contribution to the "neXt50 challenge", a coordinated effort across C-HPP teams to identify the SO most tractable missing proteins (MPs) on each chromosome. We report the targeted search of 38 theoretically detectable MPs from chromosomes 2 and 14 in Triton X-100 soluble and insoluble sperm fractions from a total of 15 healthy donors. A targeted mass spectrometry-based strategy consisting of the development of LC-PRM assays (with heavy labeled synthetic peptides) targeting 92 proteotypic peptides of the 38 selected MPs was used. Out of the 38 selected MPs, 12 were identified with two or more peptides and 3 with one peptide after extensive SDS-PAGE fractionation of the two samples and with overall low-intensity signals. The PRM data are available via ProteomeXchange in PASSEL (PASS01013). Further validation by immunohistochemistry on human testes sections and cytochemistry on sperm smears was performed for eight MPs with antibodies available from the Human Protein Atlas. Deep analysis of human sperm still allows the validation of MPs and therefore contributes to the C-HPP worldwide effort. We anticipate that our results will be of interest to the reproductive biology community because an in-depth analysis of these MPs may identify potential new candidates in the context of human idiopathic infertilities.</description>
<date>2017</date>
</dc>
</metadata>
</record>
</GetRecord>
</OAI-PMH>
