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<OAI-PMH schemaLocation=http://www.openarchives.org/OAI/2.0/ http://www.openarchives.org/OAI/2.0/OAI-PMH.xsd> <responseDate>2018-01-15T18:23:32Z</responseDate> <request identifier=oai:HAL:hal-01313006v1 verb=GetRecord metadataPrefix=oai_dc>http://api.archives-ouvertes.fr/oai/hal/</request> <GetRecord> <record> <header> <identifier>oai:HAL:hal-01313006v1</identifier> <datestamp>2018-01-11</datestamp> <setSpec>type:ART</setSpec> <setSpec>subject:sdv</setSpec> <setSpec>collection:UPMC</setSpec> <setSpec>collection:CNRS</setSpec> <setSpec>collection:UNIV-AG</setSpec> <setSpec>collection:INRA</setSpec> <setSpec>collection:MNHN</setSpec> <setSpec>collection:ICSN</setSpec> <setSpec>collection:LBBM</setSpec> <setSpec>collection:CIRAD</setSpec> <setSpec>collection:IRD</setSpec> <setSpec>collection:UNIV-TLSE3</setSpec> <setSpec>collection:AGROPARISTECH</setSpec> <setSpec>collection:ECOFOG</setSpec> <setSpec>collection:INC-CNRS</setSpec> <setSpec>collection:GUYANE</setSpec> <setSpec>collection:IFREMER</setSpec> <setSpec>collection:AGREENIUM</setSpec> <setSpec>collection:UPMC_POLE_3</setSpec> <setSpec>collection:EDF</setSpec> </header> <metadata><dc> <publisher>HAL CCSD</publisher> <title lang=en>Wayanin and guaijaverin, two active metabolites found in a Psidium acutangulum Mart. ex DC (syn. P. persoonii McVaugh) (Myrtaceae) antimalarial decoction from the Wayana Amerindians</title> <creator>Houël, Emeline</creator> <creator>Nardella, Flore</creator> <creator>Jullian, Valérie</creator> <creator>Valentin, Alexis</creator> <creator>Vonthron-Sénécheau, Catherine</creator> <creator>Villa, Pascal</creator> <creator>Obrecht, Adeline</creator> <creator>Kaiser, Marcel</creator> <creator>Bourreau, Eliane</creator> <creator>Odonne, Guillaume</creator> <creator>Fleury, Marie</creator> <creator>Bourdy, Geneviève</creator> <creator>Eparvier, Véronique</creator> <creator>Deharo, Eric</creator> <creator>Stien, Didier</creator> <contributor>Ecologie des forêts de Guyane (ECOFOG) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) - Institut National de la Recherche Agronomique (INRA) - Université des Antilles et de la Guyane (UAG) - AgroParisTech - Université de Guyane (UG) - Centre National de la Recherche Scientifique (CNRS)</contributor> <contributor>Faculté de Médecine de Strasbourg, IPPTS</contributor> <contributor>Laboratoire d'Innovation Thérapeutique (LIT) ; Centre National de la Recherche Scientifique (CNRS)</contributor> <contributor>Pharmacochimie et Pharmacologie Pour le Développement (PHARMA-DEV) ; Université Paul Sabatier - Toulouse 3 (UPS) - Institut de Recherche pour le Développement (IRD) - Centre National de la Recherche Scientifique (CNRS)</contributor> <contributor>Plateforme de chimie biologie intégrative CNRS UMS 3286 (PCBIS)</contributor> <contributor>University of Basel (Unibas)</contributor> <contributor>Swiss Tropical and Public Health Institute [Basel]</contributor> <contributor>Institut Pasteur de la Guyane</contributor> <contributor>Laboratoire Ecologie, évolution, interactions des systèmes amazoniens (LEEISA) ; Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) - Université de Guyane (UG) - Centre National de la Recherche Scientifique (CNRS)</contributor> <contributor>Patrimoines Locaux et Gouvernance (PALOC) ; Muséum National d'Histoire Naturelle (MNHN) - Institut de Recherche pour le Développement (IRD)</contributor> <contributor>Institut de Chimie des Substances Naturelles (ICSN) ; Centre National de la Recherche Scientifique (CNRS)</contributor> <contributor>Laboratoire de Biodiversité et Biotechnologies Microbiennes (LBBM) ; Université Pierre et Marie Curie - Paris 6 (UPMC) - PIERRE FABRE - Electricité de France Recherche et Développement (EDF R&D) - Centre National de la Recherche Scientifique (CNRS)</contributor> <description>International audience</description> <source>ISSN: 0378-8741</source> <source>Journal of Ethnopharmacology</source> <publisher>Elsevier</publisher> <identifier>hal-01313006</identifier> <identifier>http://hal.upmc.fr/hal-01313006</identifier> <identifier>http://hal.upmc.fr/hal-01313006/document</identifier> <identifier>http://hal.upmc.fr/hal-01313006/file/Houel_Wayanin_and.pdf</identifier> <source>http://hal.upmc.fr/hal-01313006</source> <source>Journal of Ethnopharmacology, Elsevier, 2016, 187, pp.241-248. 〈10.1016/j.jep.2016.04.053〉</source> <identifier>DOI : 10.1016/j.jep.2016.04.053</identifier> <relation>info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jep.2016.04.053</relation> <language>en</language> <subject lang=en>Psidium acutangulum</subject> <subject lang=en>Traditional remedy</subject> <subject lang=en>Cytokines</subject> <subject lang=en>Antimalarial</subject> <subject lang=en>Glycosylated flavonols</subject> <subject lang=en>French guiana</subject> <subject>[SDV.SP.PHARMA] Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology</subject> <type>info:eu-repo/semantics/article</type> <type>Journal articles</type> <description lang=en>Ethnopharmacological relevancePsidium acutangulum Mart. ex DC is a small tree used by the Wayana Amerindians from the Upper-Maroni in French Guiana for the treatment of malaria.Aim of the studyIn a previous study, we highlighted the in vitro antiplasmodial, antioxidant and anti-inflammatory potential of the traditional decoction of P. acutangulum aerial parts. Our goal was then to investigate on the origin of the biological activity of the traditional remedy, and eventually characterize active constituents.Materials and methodsLiquid-liquid extractions were performed on the decoction, and the antiplasmodial activity evaluated against chloroquine-resistant FcB1 ([3H]-hypoxanthine bioassay) and 7G8 (pLDH bioassay) P. falciparum strains, and on a chloroquine sensitive NF54 ([3H]-hypoxanthine bioassay) P. falciparum strain. The ethyl acetate fraction (D) was active and underwent bioguided fractionation. All the isolated compounds were tested on P. falciparum FcB1 strain. In vitro anti-inflammatory activity (IL-1β, IL-6, IL-8, TNFα) of the ethyl acetate fraction and of an anti-Plasmodium active compound, was concurrently assessed on LPS-stimulated human PBMC and NO secretion inhibition was measured on LPS stimulated RAW murine macrophages. Cytotoxicity of the fractions and pure compounds was measured on VERO cells, L6 mammalian cells, PBMCs, and RAW cells.ResultsFractionation of the ethyl acetate soluble fraction (IC50 ranging from 3.4 to <1 µg/mL depending on the parasite strain) led to the isolation of six pure compounds: catechin and five glycosylated quercetin derivatives. These compounds have never been isolated from this plant species. Two of these compounds (wayanin and guaijaverin) were found to be moderately active against P. falciparum FcB1 in vitro (IC50 5.5 and 6.9 µM respectively). We proposed the name wayanin during public meetings organized in June 2015 in the Upper-Maroni villages, in homage to the medicinal knowledge of the Wayana population. At 50 µg/mL, the ethyl acetate fraction (D) significantly inhibited IL-1β secretion (−46%) and NO production (−21%), as previously observed for the decoction. The effects of D and guiajaverin (4) on the secretion of other cytokines or NO production were not significant.ConclusionsThe confirmed antiplasmodial activity of the ethyl acetate soluble fraction of the decoction and of the isolated compounds support the previous results obtained on the P. acutangulum decoction. The antiplasmodial activity might be due to a mixture of moderately active non-toxic flavonoids. The anti-inflammatory activities were less marked for ethyl acetate fraction (D) than for the decoction.</description> <date>2016-07</date> </dc> </metadata> </record> </GetRecord> </OAI-PMH>