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<title lang=en>Cytochemical Investigation of the Digestive Gland of Two Strombidae Species (Strombus gigas and Strombus pugilis) in Relation to the Nutrition</title>
<creator>Volland, Jean-Marie</creator>
<creator>Gros, Olivier</creator>
<contributor>Biologie de la Mangrove (BM) ; Systématique, adaptation, évolution (SAE) ; Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Evolution Paris Seine ; Université des Antilles et de la Guyane (UAG) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Centre National de la Recherche Scientifique (CNRS) - Université des Antilles et de la Guyane (UAG) - Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA)</contributor>
<description>International audience</description>
<source>ISSN: 1059-910X</source>
<source>EISSN: 1097-0029</source>
<source>Microscopy Research and Technique</source>
<publisher>Wiley</publisher>
<identifier>hal-00743013</identifier>
<identifier>https://hal.univ-antilles.fr/hal-00743013</identifier>
<source>https://hal.univ-antilles.fr/hal-00743013</source>
<source>Microscopy Research and Technique, Wiley, 2012, 75 (10), pp.1353-1360. 〈10.1002/jemt.22074〉</source>
<identifier>DOI : 10.1002/jemt.22074</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1002/jemt.22074</relation>
<language>en</language>
<subject lang=en>molluscs</subject>
<subject lang=en>cytochemistry</subject>
<subject lang=en>physiology</subject>
<subject lang=en>lysosomal digestion</subject>
<subject lang=en>EFTEM</subject>
<subject>[SDV.BA] Life Sciences [q-bio]/Animal biology</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Strombus gigas and Strombus pugilis are threatened species and aquaculture represents a good alternative solution to the fishing. In this study, we highlighted the intracellular digestion process in the digestive gland of two Strombidae species, S. gigas and Strombus pugilis, by the cytochemical characterization of two lysosomal enzymes: acid phosphatase and arylsulfatase. In order to check the efficiency of artificial food digestion, we onducted the characterization on freshly collected, tarved and artificially fed individuals of S. pugilis. TEM observations of digestive gland sections from freshly collected individuals of both species revealed the resence of acid phosphatase and arylsulfatase activity mostly located in the apical third of digestive cells. Both enzymes were also detected in artificially fed individuals. In response to the starvation, acid phosphatase is not produced anymore by digestive cells, while arylsulfatase is still present. To our knowledge, this is the first cytochemical validation of intracellular digestion of artificial food in Strombidae. This study highlights the intracellular digestion of artificial food developed for Strombidae aquaculture. Moreover, we have shown that the lysosomal activity could be used as a feed index.</description>
<date>2012-05-25</date>
</dc>
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