untitled
<OAI-PMH schemaLocation=http://www.openarchives.org/OAI/2.0/ http://www.openarchives.org/OAI/2.0/OAI-PMH.xsd>
<responseDate>2018-01-15T18:24:56Z</responseDate>
<request identifier=oai:HAL:hal-01274885v1 verb=GetRecord metadataPrefix=oai_dc>http://api.archives-ouvertes.fr/oai/hal/</request>
<GetRecord>
<record>
<header>
<identifier>oai:HAL:hal-01274885v1</identifier>
<datestamp>2018-01-11</datestamp>
<setSpec>type:ART</setSpec>
<setSpec>subject:sdv</setSpec>
<setSpec>collection:UNIV-RENNES1</setSpec>
<setSpec>collection:CNRS</setSpec>
<setSpec>collection:UNIV-AG</setSpec>
<setSpec>collection:IRSET</setSpec>
<setSpec>collection:IGDR</setSpec>
<setSpec>collection:IRSET-VCER</setSpec>
<setSpec>collection:IRSET-PPB</setSpec>
<setSpec>collection:IFR140</setSpec>
<setSpec>collection:UNIV-TLSE3</setSpec>
<setSpec>collection:BIOSIT</setSpec>
<setSpec>collection:UR1-UFR-SVE</setSpec>
<setSpec>collection:HL</setSpec>
<setSpec>collection:UR1-HAL</setSpec>
<setSpec>collection:EHESP</setSpec>
<setSpec>collection:USPC</setSpec>
<setSpec>collection:STATS-UR1</setSpec>
<setSpec>collection:UR1-SDV</setSpec>
<setSpec>collection:IRSET-7</setSpec>
<setSpec>collection:IRSET-8</setSpec>
<setSpec>collection:UNIV-ANGERS</setSpec>
</header>
<metadata><dc>
<publisher>HAL CCSD</publisher>
<title lang=en>Comparison of the effect of semen from HIV-infected and uninfected men on CD4+ T cell infection</title>
<creator>Camus, Céline</creator>
<creator>Matusali, Giulia</creator>
<creator>Bourry, Olivier</creator>
<creator>Mahé, Dominique</creator>
<creator>Aubry, Florence</creator>
<creator>Bujan, Louis</creator>
<creator>Pasquier, Christophe</creator>
<creator>Massip, Patrice</creator>
<creator>Ravel, Célia</creator>
<creator>Zirafi, Onofrio</creator>
<creator>Münch, Jan</creator>
<creator>Roan, Nadia R.</creator>
<creator>Pineau, Charles</creator>
<creator>Dejucq-Rainsford, Nathalie</creator>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Environnement viral et chimique & reproduction ; Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) - Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>CECOS Midi-Pyrénées, centre de sterilité masculine et équipe d'accueil Fertilité Humaine ; Hôpital Paule de Viguier - EA 3694</contributor>
<contributor>Laboratoire de Virologie [Toulouse] ; CHU Toulouse [Toulouse]</contributor>
<contributor>Service des maladies infectieuses et tropicales[Toulouse] ; Université Paul Sabatier - Toulouse 3 (UPS) - CHU Toulouse [Toulouse] - Hôpital Purpan [Toulouse]</contributor>
<contributor>Institut de Génétique et Développement de Rennes (IGDR) ; Université de Rennes 1 (UR1) - Centre National de la Recherche Scientifique (CNRS) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>CHU Pontchaillou [Rennes]</contributor>
<contributor>Institute of Molecular Virology ; University Hospital Ulm</contributor>
<description>International audience</description>
<source>AIDS</source>
<identifier>hal-01274885</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01274885</identifier>
<source>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01274885</source>
<source>AIDS, 2016, 30 (8), pp.1197-1208. 〈10.1097/QAD.0000000000001048〉</source>
<identifier>DOI : 10.1097/QAD.0000000000001048</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1097/QAD.0000000000001048</relation>
<identifier>PUBMED : 26854806</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/26854806</relation>
<language>en</language>
<subject>[SDV] Life Sciences [q-bio]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>OBJECTIVES: Semen composition is influenced by HIV-1 infection, yet the impact of semen components on HIV infection of primary target cells has only been studied in samples from HIV-uninfected donors. DESIGN: We compared the effect of seminal plasma from chronically HIV-infected (SP+) versus uninfected donors (SP-) on HIV-1 infection of PBMCs and CD4+T cells. METHODS: Primary cells were infected with HIV-1 in the presence of SP+ or SP- and analyzed for infection level, metabolic activity, HIV receptor expression, proliferation and activation. SP+ and SP- were compared for infection-enhancing peptides, cytokines and prostaglandin E2 levels. RESULTS: SP- efficiently enhanced HIV-1 R5 infection of CD4+ T cells, whereas SP+ enhancing activity was significantly reduced. RANTES concentrations were elevated in SP+ relative to SP-, while the concentrations of infectivity-enhancing peptides (SEVI, SEM1, SEM2) were similar. CCR5 membrane expression levels were reduced on CD4+T cells shortly post-exposure to SP+ compared with SP- and correlated to R5-tropic HIV-1 infection levels, and CCR5 ligands' concentrations in semen. SP+ and SP- displayed similar enhancing activity on PBMC infection by X4-tropic HIV-1. Addition/depletion of RANTES from seminal plasmas modulated their effect on PBMC infection by R5-tropic HIV-1. CONCLUSIONS: Semen from HIV-infected donors exhibit a significantly reduced enhancing potential on CD4+ T cell infection by R5-tropic HIV-1 when compared with semen from uninfected donors. Our data indicate that elevated seminal concentrations of RANTES in HIV+ men can influence the ability of semen to enhance infection.This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives 4.0 License, where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially. http://creativecommons.org/licenses/by-nc-nd/4.0</description>
<date>2016-02</date>
</dc>
</metadata>
</record>
</GetRecord>
</OAI-PMH>
