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<datestamp>2018-01-12</datestamp>
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<title lang=en>Exploration of Deinococcus-Thermus molecular diversity by novel group-specific PCR primers</title>
<creator>Theodorakopoulos, Nicolas</creator>
<creator>Bachar, Dipankar</creator>
<creator>Christen, Richard</creator>
<creator>Alain, Karine</creator>
<creator>Chapon, Virginie</creator>
<contributor>Biologie végétale et microbiologie environnementale - UMR7265 (BVME) ; Centre National de la Recherche Scientifique (CNRS) - Commissariat à l'énergie atomique et aux énergies alternatives (CEA) - Aix Marseille Université (AMU)</contributor>
<contributor>Symbiose Marine (SM) ; Systématique, adaptation, évolution (SAE) ; Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Evolution Paris Seine ; Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Centre National de la Recherche Scientifique (CNRS) - Université des Antilles et de la Guyane (UAG) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Université des Antilles et de la Guyane (UAG)</contributor>
<contributor>Laboratoire de microbiologie des environnements extrêmophiles (LM2E) ; Centre National de la Recherche Scientifique (CNRS) - Université de Brest (UBO) - Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)</contributor>
<contributor>CEA - Toxicology programme</contributor>
<description>International audience</description>
<source>ISSN: 2045-8827</source>
<source>MicrobiologyOpen</source>
<publisher>Wiley</publisher>
<identifier>hal-01560945</identifier>
<identifier>https://hal-amu.archives-ouvertes.fr/hal-01560945</identifier>
<identifier>https://hal-amu.archives-ouvertes.fr/hal-01560945/document</identifier>
<identifier>https://hal-amu.archives-ouvertes.fr/hal-01560945/file/Theodorakopoulos-2013.pdf</identifier>
<source>https://hal-amu.archives-ouvertes.fr/hal-01560945</source>
<source>MicrobiologyOpen, Wiley, 2013, 2 (5), pp.862-872. 〈http://onlinelibrary.wiley.com/doi/10.1002/mbo3.119/abstract〉. 〈10.1002/mbo3.119〉</source>
<identifier>DOI : 10.1002/mbo3.119</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1002/mbo3.119</relation>
<source>http://onlinelibrary.wiley.com/doi/10.1002/mbo3.119/abstract</source>
<language>en</language>
<subject lang=en> group-specific primers</subject>
<subject lang=en>molecular diversity</subject>
<subject lang=en>Deinococcus-Thermus</subject>
<subject>[SDV] Life Sciences [q-bio]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>The deeply branching Deinococcus-Thermus lineage is recognized as one of the most extremophilic phylum of bacteria. In previous studies, the presence of Deinococcus-related bacteria in the hot arid Tunisian desert of Tataouine was demonstrated through combined molecular and culture-based approaches. Similarly , Thermus-related bacteria have been detected in Tunisian geothermal springs. The present work was conducted to explore the molecular diversity within the Deinococcus-Thermus phylum in these extreme environments. A set of specific primers was designed in silico on the basis of 16S rRNA gene sequences, validated for the specific detection of reference strains, and used for the polymerase chain reaction (PCR) amplification of metagenomic DNA retrieved from the Tataouine desert sand and Tunisian hot spring water samples. These analyses have revealed the presence of previously undescribed Deino-coccus-Thermus bacterial sequences within these extreme environments. The primers designed in this study thus represent a powerful tool for the rapid detection of Deinococcus-Thermus in environmental samples and could also be applicable to clarify the biogeography of the Deinococcus-Thermus phylum.</description>
<rights>http://creativecommons.org/licenses/by/</rights>
<date>2013-08-29</date>
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