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<identifier>oai:HAL:hal-01031865v1</identifier>
<datestamp>2018-01-11</datestamp>
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<metadata><dc>
<publisher>HAL CCSD</publisher>
<title lang=en>Efficient development of dinucleotide microsatellite markers in Norway spruce (Picea abies Karst.) through dot blot selection</title>
<creator>Scotti, Ivan</creator>
<creator>Paglia, Gianpaolo</creator>
<creator>Magni, Federica</creator>
<creator>Morgante, Michele</creator>
<contributor>Ecologie des forêts de Guyane (ECOFOG) ; Ecole Nationale du Génie Rural, des Eaux et des Forêts (ENGREF) - Institut National de la Recherche Agronomique (INRA) - Université des Antilles et de la Guyane (UAG) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Dipartimento di Scienze Agrarie ed Ambientali ; Università degli Studi di Udine - University of Udine [Italie]</contributor>
<source>Theoretical and Applied Genetics</source>
<publisher>SpringerLink</publisher>
<identifier>hal-01031865</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01031865</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01031865</source>
<source>Theoretical and Applied Genetics, SpringerLink, 2002, 104 (6-7), pp.1035-1041. 〈10.1007/s00122-001-0843-7〉</source>
<identifier>DOI : 10.1007/s00122-001-0843-7</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1007/s00122-001-0843-7</relation>
<language>en</language>
<subject lang=fr>SSR</subject>
<subject lang=fr>DOT-BLOT</subject>
<subject lang=fr>MOLECULAR MARKERS</subject>
<subject lang=fr>ENRICHMENT</subject>
<subject lang=fr>EPICEA COMMUN</subject>
<subject>[SDV.GEN] Life Sciences [q-bio]/Genetics</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>The development of microsatellite markers can be a time-consuming process, especially in species such as conifers where many microsatellites have been shown to be associated with the repetitive fraction of the genome and to produce complex banding patterns following electrophoresis. Therefore, procedures to eliminate this fraction from further processing are sought. In this paper, we report on the development of 53 dinucleotide SSR markers in Norway spruce, 35 of which (66%) produce simple, polymorphic patterns. This high efficiency is obtained by introducing a dot-blot selection against high copy number sequences, performed on the microsatellite-containing clones. The resulting markers turned out to be polymorphic and useful for population genetic studies and for linkage mapping. Seven additional markers that were not subject to the dot-blot selection are also presented.</description>
<date>2002</date>
</dc>
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