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<identifier>oai:HAL:hal-01031904v1</identifier>
<datestamp>2018-01-11</datestamp>
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<publisher>HAL CCSD</publisher>
<title lang=en>Evaluation of quantitative and qualitative recovery of bacterial communities from different soil types by density gradient centrifugation</title>
<creator>Maron, Pierre-Alain</creator>
<creator>Schimann, Heidy</creator>
<creator>Ranjard, Lionel</creator>
<creator>Brothier, Elisabeth</creator>
<creator>Domenach, Anne-Marie</creator>
<creator>Lensi, Robert</creator>
<creator>Nazaret, Sylvie</creator>
<contributor>Microbiologie du Sol et de l'Environnement (MSE) ; Institut National de la Recherche Agronomique (INRA) - Université de Bourgogne (UB)</contributor>
<contributor>Ecologie des forêts de Guyane (ECOFOG) ; Ecole Nationale du Génie Rural, des Eaux et des Forêts (ENGREF) - Institut National de la Recherche Agronomique (INRA) - Université des Antilles et de la Guyane (UAG) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Ecologie microbienne (EM) ; Institut National de la Recherche Agronomique (INRA) - Université Claude Bernard Lyon 1 (UCBL) - Ecole Nationale Vétérinaire de Lyon (ENVL) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<source>ISSN: 1164-5563</source>
<source>European Journal of Soil Biology</source>
<publisher>Elsevier</publisher>
<identifier>hal-01031904</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01031904</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01031904</source>
<source>European Journal of Soil Biology, Elsevier, 2006, 42 (2), pp.65-73. 〈10.1016/j.ejsobi.2005.08.003〉</source>
<identifier>DOI : 10.1016/j.ejsobi.2005.08.003</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejsobi.2005.08.003</relation>
<language>en</language>
<subject lang=en>BACTERIAL COMMUNITY</subject>
<subject lang=en>SOIL</subject>
<subject lang=en>DENSITY GRADIENT</subject>
<subject lang=en>DNA FINGERPRINT</subject>
<subject>[SDV.SA] Life Sciences [q-bio]/Agricultural sciences</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Extracting and purifying a representative fraction of bacteria from soil is necessary for the application of many techniques of microbial ecology. Here the influence of different soil types on the quantitative and qualitative recovery of bacteria by soil grinding and Nycodenz density gradient centrifugation was investigated. Three soils presenting contrasted physicochemical characteristics were used for this study. For each soil, the total (AODC: acridine orange direct count) and culturable (cfu: colony-forming units) bacterial densities were measured in three distinct fractions: (i) the primary soil, (ii) the soil pellet (soil remaining after centrifugation), and (iii) the extracted cells. The automated-ribosomal intergenic spacer analysis (A-RISA) was used to characterize the community structure directly from the DNA extracted from each fraction. The physicochemical characteristics of soils were found to influence both the efficiency of bacterial cell recovery and the representativeness of the extracted cells in term of community structures between the different fractions. Surprisingly, the most representative extracted cells were obtained from the soil exhibiting the lowest efficiency of cell recovery. Our results demonstrated that quantitative and qualitative cell recovery using Nycodenz density gradient centrifugation are not necessarily related and could be differentially biased according to soil type.</description>
<date>2006</date>
</dc>
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