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<publisher>HAL CCSD</publisher>
<title lang=en>Extracellular DNA extraction is a fast, cheap and reliable alternative for multi-taxa surveys based on soil DNA</title>
<creator>Zinger, Lucie</creator>
<creator>Chave, Jérôme</creator>
<creator>Coissac, Eric</creator>
<creator>Iribar, Amaia</creator>
<creator>Louisanna, Eliane</creator>
<creator>Manzi, Sophie</creator>
<creator>Schilling, Vincent</creator>
<creator>Schimann, Heidy</creator>
<creator>Sommeria-Klein, Guilhem</creator>
<creator>Taberlet, Pierre</creator>
<contributor>UMR 5174 ; Université Paul Sabatier - Toulouse 3 (UPS)</contributor>
<contributor>Laboratoire d'Ecologie Alpine (LECA) ; Université Joseph Fourier - Grenoble 1 (UJF) - Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>Ecologie des forêts de Guyane (ECOFOG) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) - Institut National de la Recherche Agronomique (INRA) - Université des Antilles et de la Guyane (UAG) - AgroParisTech - Université de Guyane (UG) - Centre National de la Recherche Scientifique (CNRS)</contributor>
<contributor>UMR 5144 ; Université Paul Sabatier - Toulouse 3 (UPS)</contributor>
<contributor>Projet Metabar ANR 11 BSV7 0020 ; Investissement d'avenir ANR 10 LABX 2501 ; LABX 0041 ; ANR 11 INBS 0001</contributor>
<source>ISSN: 0038-0717</source>
<source>Soil Biology and Biochemistry</source>
<publisher>Elsevier</publisher>
<identifier>hal-01532638</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-01532638</identifier>
<source>https://hal.archives-ouvertes.fr/hal-01532638</source>
<source>Soil Biology and Biochemistry, Elsevier, 2016, 96, pp.16-19. 〈10.1016/j.soilbio.2016.01.008〉</source>
<identifier>DOI : 10.1016/j.soilbio.2016.01.008</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1016/j.soilbio.2016.01.008</relation>
<identifier>PRODINRA : 384522</identifier>
<language>en</language>
<subject lang=en>DNA metabarcoding</subject>
<subject lang=en>DNA extraction protocol</subject>
<subject lang=en>tropical forest</subject>
<subject lang=en>multi-taxa biodiversity</subject>
<subject lang=en>diversity</subject>
<subject lang=fr>biodiversité</subject>
<subject lang=fr>diversité</subject>
<subject lang=fr>communauté</subject>
<subject lang=fr>fungi</subject>
<subject>[SDV] Life Sciences [q-bio]</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>DNA metabarcoding on soil samples is increasingly used for large-scale and multi-taxa biodiversity studies. However, DNA extraction may be a major bottleneck for such wide uses. It should be cost/time effective and allow dealing with large sample volumes so as to maximise the representativeness of both micro- and macro-organisms diversity. Here, we compared the performances of a fast and cheap extracellular DNA extraction protocol with a total DNA extraction method in retrieving bacterial, eukaryotic and plant diversity from tropical soil samples of ca. 10 g. The total DNA extraction protocol yielded more high-quality DNA. Yet, the extracellular DNA protocol provided similar diversity assessments although it presented some differences in clades relative abundance and undersampling biases. We argue that extracellular DNA is a good compromise between cost, labor, and accuracy for high throughput DNA metabarcoding studies of soil biodiversity.</description>
<date>2016</date>
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