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<datestamp>2015-02-18</datestamp>
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<publisher>HAL CCSD</publisher>
<title lang=en>Human immunodeficiency virus infects human seminal vesicles in vitro and in vivo.</title>
<creator>Deleage, Claire</creator>
<creator>Moreau, Marina</creator>
<creator>Rioux-Leclercq, Nathalie</creator>
<creator>Ruffault, Annick</creator>
<creator>Jégou, Bernard</creator>
<creator>Dejucq-Rainsford, Nathalie</creator>
<contributor>Groupe d'Etude de la Reproduction Chez l'Homme et les Mammiferes (GERHM) ; INSERM - IFR140 - Université de Rennes 1 (UR1) - Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; INSERM - École Nationale de la Santé Publique - Université de Rennes 1 (UR1) - Université des Antilles et de la Guyane (UAG) - Structure Fédérative de Recherche en Biologie-Santé de Rennes (Biosit) ; Université de Rennes 1 (UR1) - INSERM - CNRS - INSERM - CNRS - INSERM - École Nationale de la Santé Publique - Université des Antilles et de la Guyane (UAG) - Structure Fédérative de Recherche en Biologie-Santé de Rennes (Biosit) ; Université de Rennes 1 (UR1) - INSERM - CNRS - INSERM - CNRS</contributor>
<contributor>Service d'anatomie et cytologie pathologiques ; Université de Rennes 1 (UR1) - Hôpital Pontchaillou - CHU Rennes</contributor>
<contributor>Unité de Rétrovirologie ; Hôpital Pontchaillou</contributor>
<contributor>Supported by grant 5 U42 RR006042 from the National Institutes of Health and by INSERM, ANRS, Sidaction and Région Bretagne.</contributor>
<description>International audience</description>
<source>American Journal of Pathology</source>
<publisher>American Society for Investigative Pathology (ASIP)</publisher>
<identifier>inserm-00626549</identifier>
<identifier>http://www.hal.inserm.fr/inserm-00626549</identifier>
<source>http://www.hal.inserm.fr/inserm-00626549</source>
<source>American Journal of Pathology, American Society for Investigative Pathology (ASIP), 2011, 179 (5), pp.2397-408. <10.1016/j.ajpath.2011.08.005></source>
<identifier>DOI : 10.1016/j.ajpath.2011.08.005</identifier>
<identifier>PUBMED : 21925468</identifier>
<language>en</language>
<subject>[SDV.BDLR] Life Sciences/Reproductive Biology</subject>
<subject>[SDV.MP] Life Sciences/Microbiology and Parasitology</subject>
<type>Journal articles</type>
<description lang=en>Semen represents the main vector of HIV dissemination worldwide, yet the origin of HIV in semen remains unclear. Viral populations distinct from those found in blood have been observed in semen, indicating local viral replication within the male genital tract. The seminal vesicles, the secretions of which constitute more than 60% of the seminal fluid, could represent a major source of virus in semen. This study is the first to investigate the susceptibility of human seminal vesicles to HIV infection both in vitro and in vivo. We developed and characterized an organotypic culture of human seminal vesicles to test for target cells and HIV infection, and, in parallel, analyzed the seminal vesicle tissues from HIV-infected donors. In vitro, in contrast to HIV-1 X4, HIV-1 R5 exposure induced productive infection. Infected cells consisted primarily of resident CD163(+) macrophages, often located close to the lumen. In vivo, HIV protein and RNA were also detected primarily in seminal vesicle macrophages in seven of nine HIV-infected donors, some of whom were receiving prolonged suppressive highly active antiretroviral therapy. These results demonstrate that human seminal vesicles support HIV infection in vitro and in vivo and, therefore, have the potential to contribute virus to semen. The presence of infected cells in the seminal vesicles of treated men with undetectable viremia suggests that this organ could constitute a reservoir for HIV.</description>
<date>2011-11</date>
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