Chemical Purification, Analysis and Screening core facility: overcoming challenges of natural product research in drug discovery Auteur(s) : Calcul, Laurent Année de publication : Loading the player... Éditeur(s) : Université des Antilles AREBio Groupe de recherche BIOSPHERES : BIOlogie, Sciences Physiques & Humaines pour les énergies Renouvelables, l Extrait de : 1er colloque international BIOSPHERES, du 18 au 20 juin 2019. Université des Antilles Description : The Chemical Purification, analysis and Screening core laboratories (CPAS) are located at the University of South Florida campus in Tampa. This core offers instrumentation and expertise in fractionation, purification, and various chemical analyses to support synthetic and natural products drug discovery research groups. Our main equipment are dedicated to chromatographic separation and purification (MPLC, HPLC) and mass-spectrometry for analytic detection, quantification and identification (LC-MS SQ, QqQ and QToF). The CPAS services also include Circular Dichroism spectrometry, general spectroscopic characterization (UV, FTIR), lyophilization (bench top and high capacity freeze-dryers), synthesis (microwave reactor), ADME screening (permeability and drug solubility testing), and high-throughput screening development capability (automated liquid handler and multimode plate reader). An example of three significant studies will be presented at the conference to illustrate the CPAS core facility services: 1) Identification of new bioactive compounds from mangrove endophytic fungi as potential alternatives to malaria drug resistance, 2) Myricanol isolated from Bayberry (Myrica Cerifera) and its synthetic derivatives targeting the microtubuleassociated protein tau accumulation in the Alzheimer's disease and 3) New ent-labdane diterpenes with solid tumor cell lines antiproliferative properties from the endemic plant Eupatorium obtusissmum from the island of Hispaniola. The core provides research lab training workshops in advanced technology and assistance so users can operate any instruments available in the laboratories to successfully accomplish their analyses and research projects. Permalien : http://www.manioc.org/fichiers/V19063 | Partager |
Willingness of south Florida fruit growers to adopt genetically modified papaya : an ex-ante evaluation Auteur(s) : Evans, edward Année de publication : Loading the player... Éditeur(s) : INRA : Institut National de la Recherche Agronomique Université des Antilles. Service commun de la documentation University of Florida Extrait de : 52e congrès annuel de la Société caribéenne des plantes alimentaires / 52nd annual meeting of the Caribbean food crops society (CFCS), du 10 au 16 juillet 2016. INRA, CFCS Description : Papaya ringspot virus (PRSV) has been a major factor inhibiting the expansion of papaya production in South Florida, USA. The University of Florida has developed a transgenic papaya line X17-2, which is tolerant to PRSV and is suitable for commercial production. The expected yield improvement can be as high as 380%. In spite of the yield potential, some fruit growers may be skeptical about adopting this improved GM cultivars for a variety of reasons. Consequently, a probit model with data from a survey of likely producers was used to identify those factors that may influence the adoption of the new technology. The results indicate that education level, farm size, and income derived from agriculture were among the factors likely to increase adoption of the technology. The findings suggest that a targeted education program will go a far way in increasing rate of adoption of the technology. Le virus de tache annulaire de papaye (PRSV) a été un facteur important empêchant l'expansion de la production de papaye en Floride du sud, Etats-Unis. L'université de la Floride a développé une ligne transgénique X17-2 de papaye, qui est tolérante à PRSV et convient à la production commerciale. L'amélioration prévue de rendement peut être aussi haute que 380%. Malgré le potentiel de rendement, quelques arboriculteurs peuvent être sceptiques au sujet d'adopter ceci les cultivars améliorés de GM pour des raisons diverses. En conséquence, un modèle de probit avec des données d'une enquête des producteurs probables a été employé pour identifier ces facteurs qui peuvent influencer l'adoption de la nouvelle technologie. Les résultats indiquent que le niveau d'éducation, la taille de ferme, et le revenu dérivé de l'agriculture étaient parmi les facteurs vraisemblablement pour augmenter l'adoption de la technologie. Les résultats suggèrent qu'un programme éducatif visé aille une manière lointaine dans le taux croissant d'adoption de la technologie. Siècle(s) traité(s) : 21 Permalien : http://www.manioc.org/fichiers/V16254 | Partager |