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<publisher>HAL CCSD</publisher>
<title lang=en>Molecular diagnosis of toxoplasmosis in immunocompromised patients</title>
<creator>Robert-Gangneux, Florence</creator>
<creator>Belaz, Sorya</creator>
<contributor>Service de Parasitologie-Mycologie [Rennes] ; Université de Rennes 1 (UR1) - Hôpital Pontchaillou - CHU Pontchaillou [Rennes]</contributor>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<description>International audience</description>
<source>ISSN: 0951-7375</source>
<source>Current Opinion in Infectious Diseases</source>
<publisher>Lippincott, Williams & Wilkins</publisher>
<identifier>hal-01390970</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01390970</identifier>
<source>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01390970</source>
<source>Current Opinion in Infectious Diseases, Lippincott, Williams & Wilkins, 2016, 29 (4), pp.330--339. 〈10.1097/QCO.0000000000000275〉</source>
<identifier>DOI : 10.1097/QCO.0000000000000275</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1097/QCO.0000000000000275</relation>
<identifier>PUBMED : 27191201</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/27191201</relation>
<language>en</language>
<subject lang=en>polymerase-chain-reaction</subject>
<subject lang=en> real-time pcr</subject>
<subject lang=en> goldmann-witmer coefficient</subject>
<subject lang=en> stem-cell transplantation</subject>
<subject lang=en> isothermal amplification lamp</subject>
<subject lang=en> transfer hybridization probes</subject>
<subject lang=en> peripheral-blood samples</subject>
<subject lang=en> amniotic-fluid samples</subject>
<subject lang=en> focal brain-lesions</subject>
<subject lang=en> ocular toxoplasmosis</subject>
<subject>[SDV.EE.SANT] Life Sciences [q-bio]/Ecology, environment/Health</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Purpose of review Toxoplasmosis in immunocompromised patients is associated with a high mortality rate. Molecular techniques are important tools to diagnose acute disease in immunocompromised patients, but there are various methods with variable efficiency. Some of them have been validated for the diagnosis of congenital toxoplasmosis, but the impact of their use has not been evaluated in immunocompromised patients. Recent findings Toxoplasmosis is of increasing importance in non-HIV immunocompromised patients. In addition, the picture of disease shows greater severity in South America, both in immunocompetent study participants and in congenitally infected infants. These epidemiological differences could influence the sensitivity of diagnostic methods. This review analyzes recent data on molecular diagnosis and compares them with older ones, in light of progress gained in molecular techniques and of recent epidemiological findings. Most recent studies were conducted in South America and used PCR targeting the B1 gene. PCR on blood could allow diagnosing a significant proportion of patients with ocular toxoplasmosis in Brazil. Summary Quantitative PCR methods with specific probes should be used to improve sensitivity and warrant specificity. Performance of quantitative PCR targeting the repeated 529 bp sequence for the diagnosis of toxoplasmosis in immunocompromised patients needs evaluation in field studies in South America and in western countries.</description>
<date>2016</date>
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