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<datestamp>2018-01-11</datestamp>
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<publisher>HAL CCSD</publisher>
<title lang=en>Clear cell renal cell carcinoma: a comparative study of histological and chromosomal characteristics between primary tumors and their corresponding metastases</title>
<creator>Dagher, Julien</creator>
<creator>Kammerer-Jacquet, Solène-Florence</creator>
<creator>Dugay, Frédéric</creator>
<creator>Beaumont, Marion</creator>
<creator>Lespagnol, Alexandra</creator>
<creator>Cornevin, Laurence</creator>
<creator>Verhoest, Grégory</creator>
<creator>Bensalah, Karim</creator>
<creator>Rioux-Leclercq, Nathalie</creator>
<creator>Belaud-Rotureau, Marc-Antoine</creator>
<contributor>Service de Cytogénétique et de Biologie Cellulaire ; Université de Rennes 1 (UR1) - Hôpital Pontchaillou - CHU Pontchaillou [Rennes]</contributor>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Service d'anatomie et cytologie pathologiques [Rennes] ; Université de Rennes 1 (UR1) - Hôpital Pontchaillou - CHU Pontchaillou [Rennes]</contributor>
<contributor>Institut de Génétique et Développement de Rennes (IGDR) ; Université de Rennes 1 (UR1) - Centre National de la Recherche Scientifique (CNRS) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>CHU Pontchaillou [Rennes]</contributor>
<contributor>Service d'urologie ; Université de Rennes 1 (UR1) - Hôpital Pontchaillou - CHU Pontchaillou [Rennes]</contributor>
<description>International audience</description>
<source>Virchows Archiv</source>
<identifier>hal-01560281</identifier>
<identifier>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560281</identifier>
<source>https://hal-univ-rennes1.archives-ouvertes.fr/hal-01560281</source>
<source>Virchows Archiv, 2017, 471 (1), pp.107-115. 〈10.1007/s00428-017-2124-0〉</source>
<identifier>DOI : 10.1007/s00428-017-2124-0</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1007/s00428-017-2124-0</relation>
<identifier>PUBMED : 28488172</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/28488172</relation>
<language>en</language>
<subject lang=en> Chromosome</subject>
<subject lang=en> clear cell renal cell carcinoma</subject>
<subject lang=en> metastasis</subject>
<subject lang=en>CGH</subject>
<subject>[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>Clear cell renal cell carcinoma (ccRCC) has a poor prognosis with a 50% risk of metastases. Little is known about the phenotypic and molecular profiles of metastases regarding their corresponding primary tumors. This study aimed to screen phenotypic and genotypic differences between metastases and their corresponding primary tumors. We selected four cases with available frozen material. The histological, immunohistochemical (VEGFA, CD31, SMA, Ki67, p53, PAR-3), FISH (VHL gene), next-generation sequencing (VHL and c-MET genes), multiplex ligation-dependent probe amplification, and array-(comparative genomic hybridization) CGH analyses were realized. Metastases were nodal, hepatic (synchronous), adrenal, and pulmonary (metachronous). High-grade tumor cells were significantly more frequent in metastases (p = 0.019). Metastases and high-grade zones of primary tumors shared similar characteristics compared to low-grade zones: a lower microscopic vascular density (43.5 vs 382.5 vessels/mm(2); p = 0.0027), a higher expression of VEGF (73 vs 10%, p = 0.045), Ki67 (37.6 vs 8.3%; p = 0.011), and p53 (54 vs 10.6%; p = 0.081), and a cytoplasmic and membranous PAR-3 staining. Metastases exhibited more chromosomal imbalances than primary tumors in total (18.75 ± 6.8; p = 0.044) with more genomic gains (13.5 ± 7; p = 0.013). The loss of chromosome 9 and gain of Xq were found in both primary tumors and metastases but gains of loci or chromosomes 2p, 3q, 5, 8q, 12, and 20 were only found in metastases. The VHL gene status was similar in each tumor couple. Although metastases and primary tumors share common histological features, this study highlights chromosomal differences specific to metastases which could be involved in ccRCC metastatic evolution.</description>
<date>2017</date>
</dc>
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