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<title lang=en>Expression of HLA-G by mast cells is associated with hepatitis C virus-induced liver fibrosis.</title>
<creator>Amiot, Laurence</creator>
<creator>Vu, Nicolas</creator>
<creator>Rauch, Michel</creator>
<creator>Helgoualc'H, Annie L'</creator>
<creator>Chalmel, Frédéric</creator>
<creator>Gascan, Hugues</creator>
<creator>Turlin, Bruno</creator>
<creator>Guyader, Dominique</creator>
<creator>Samson, Michel</creator>
<contributor>Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Transcriptional networks in gametogenesis and cancer ; Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) - Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Institut de Génétique et Développement de Rennes (IGDR) ; Université de Rennes 1 (UR1) - Centre National de la Recherche Scientifique (CNRS) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )</contributor>
<contributor>Département d'Anatomie et Cytologie Pathologiques ; Hôpital Pontchaillou - CHU Pontchaillou [Rennes]</contributor>
<contributor>Service d'hépato- gastro-entérologie ; Université de Rennes 1 (UR1) - Hôpital Pontchaillou - CHU Pontchaillou [Rennes]</contributor>
<contributor>This work was supported by grants from ANRS 2010, Ligue Nationale Contre le Cancer (Comité d'Ille et Vilaine) 2009, IFR 140 2010, Biosit 2012. We thank Dr Butterfield for his generous gift of the human mast cell line HMC1.1, Dr Geraghty for providing LCL721.221.G5, the Biological Resource Center (BRC) of Rennes University Hospital for providing liver biopsies, the H2P2 platform of Biosit and particularly Pascale Bellaud for technical assistance. We also thank Ahmed Zahedi of Rennes University Hospital for technical assistance.</contributor>
<description>International audience</description>
<source>ISSN: 0168-8278</source>
<source>EISSN: 0168-8278</source>
<source>Journal of Hepatology</source>
<publisher>Elsevier</publisher>
<identifier>hal-00874106</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-00874106</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-00874106/document</identifier>
<identifier>https://hal.archives-ouvertes.fr/hal-00874106/file/Amiot-Expression-HLA-G-revised.pdf</identifier>
<source>https://hal.archives-ouvertes.fr/hal-00874106</source>
<source>Journal of Hepatology, Elsevier, 2013, 60 (2), pp.245-252. 〈10.1016/j.jhep.2013.09.006〉</source>
<identifier>DOI : 10.1016/j.jhep.2013.09.006</identifier>
<relation>info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jhep.2013.09.006</relation>
<identifier>PUBMED : 24036009</identifier>
<relation>info:eu-repo/semantics/altIdentifier/pmid/24036009</relation>
<language>en</language>
<subject lang=en>Hepatitis C</subject>
<subject lang=en>HCV</subject>
<subject lang=en>Liver</subject>
<subject lang=en>Fibrosis</subject>
<subject lang=en>HLA-G</subject>
<subject lang=en>Mast cell</subject>
<subject lang=en>IFN-α</subject>
<subject>[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases</subject>
<type>info:eu-repo/semantics/article</type>
<type>Journal articles</type>
<description lang=en>BACKGROUND AND AIMS: Infection by hepatitis C virus is a worldwide health problem. An inadequate Th2 cytokine response promotes the fibrosis-cirrhosis fate. Immune-modulating molecules favoring a Th2 profile, such as HLA-G molecules of the HLA class Ib family, may play a role in chronic hepatitis. HLA-G contributes to the escape of tumors, and their involvement in viral infections has been increasingly described. The aim of this work was to study the expression of HLA-G in the liver, its cellular source and its regulation in cases of chronic C hepatitis. METHODS: HLA-G cells in blocks of liver derived from patients infected with HCV were labeled by immunohistochemistry and enumerated. Double immunofluorescence allowed the identification of the cellular source. HLA-G secretion by a human mast cell line was quantified by ELISA after various stimulations. After treatment with IFN-α real-time PCR was performed to determine the kinetics of cytokine expression profiles, followed by heat map clustering analysis. RESULTS: The number of HLA-G + cells was significantly associated with the area of fibrosis. For the first time, we identify the HLA-G+ cells as being mast cells. HLA-G secretion was significantly induced in human mast cells stimulated by IL-10 or interferons of class I. The transcriptome of the secretome of this cell line stimulated by IFN-α revealed that i) the HLA-G gene is upregulated late, ii) T lymphocytes and NK cells are recruited. CONCLUSIONS: These findings suggest an autocrine loop in the genesis of HCV liver fibrosis, based on mast cells expressing HLA-G.</description>
<date>2013-09-11</date>
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