Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
A new genus and species of deep-sea eelpout (Zoarcidae), Barbapellis pterygalces, is herein described from a single adult female specimen carrying eggs and measuring 204 mm in total length. It was collected on the eastern Antarctic continental slope, off Terre Ad,lie, at ca. 1,200 m depth, on muddy bottom. Barbapellis is placed in the subfamily Gymnelinae and can be distinguished from all other genera by morphological characters unusual in zoarcids as well as by molecular barcoding. The genus is characterized by the presence of a complex system of cephalic skin crests and flaps, especially well developed around the mouth. A new terminology is proposed for the eleven crests and sevenfolds described. The skin folds may be used for foraging on muddy sea bottoms. The genus is also characterized by large, flexible, and highly palmate pectoral fins. The designation of the new genus is discussed.
ISSN: 0722-4060

hal-01544913
https://hal.archives-ouvertes.fr/hal-01544913
DOI : 10.1007/s00300-011-1057-3

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Background: Researchers sorely need markers and approaches for biodiversity exploration (both specimen linked and metagenomics) using the full potential of next generation sequencing technologies (NGST). Currently, most studies rely on expensive multiple tagging, PCR primer universality and/or the use of few markers, sometimes with insufficient variability. Methodology/Principal Findings: We propose a novel approach for the isolation and sequencing of a universal, useful and popular marker across distant, non-model metazoans: the complete mitochondrial genome. It relies on the properties of metazoan mitogenomes for enrichment, on careful choice of the organisms to multiplex, as well as on the wide collection of accumulated mitochondrial reference datasets for post-sequencing sorting and identification instead of individual tagging. Multiple divergent organisms can be sequenced simultaneously, and their complete mitogenome obtained at a very low cost. We provide in silico testing of dataset assembly for a selected set of example datasets. Conclusions/Significance: This approach generates large mitogenome datasets. These sequences are useful for phylogenetics, molecular identification and molecular ecology studies, and are compatible with all existing projects or available datasets based on mitochondrial sequences, such as the Barcode of Life project. Our method can yield sequences both from identified samples and metagenomic samples. The use of the same datasets for both kinds of studies makes for a powerful approach, especially since the datasets have a high variability even at species level, and would be a useful complement to the less variable 18S rDNA currently prevailing in metagenomic studies.
ISSN: 1932-6203

hal-01544766
https://hal.archives-ouvertes.fr/hal-01544766
DOI : 10.1371/journal.pone.0051263