Éditeur(s) : HAL CCSD Elsevier Masson
Résumé : Fire-free forest conversion with organic inputs as an alternative to slash-and-burn could improve agro-ecosystem sustainability. We assessed soil carbon mass changes in a sandy-clayey and well-drained soil in French Guiana after forest clearing by the chop-and-mulch method and crop establishment. At the experimental site of Combi, native forest was cut down in October 2008; woody biomass was chopped and incorporated into the top 20 cm of soil. After about one year of legume and grass cover, three forms of land management were compared: grassland (Urochloa ruziziensis), maize/soybean crop rotation with disk tillage and in direct seeding without tillage. There were four replicates. We measured 14.16 kg m(-2) of carbon in 2 mm-sieved soil down to 2 m depth for the initial forest. Forest clearing did not induce significant soil compaction; neither did any specific agricultural practice. In converted soils, C stocks were measured in the 0-30 cm layer after each crop for three years. Carbon mass changes for soil fractions <2 mm (soil C stock) and >2 mm (soil C pool) in the 0-5, 5-10, 10-20 and 20-30 cm soil layers were assessed on an equivalent soil mass basis. One year and 1.5 years after deforestation, higher C stocks (+0.64 to 1.16 kg C m(-2) yr(-1)) and C pools (+0.52 to 0.90 kg C m(-2) yr(-1)) were measured in converted soils, compared to those of the forest into the top 30 cm of soil. However, the masses of carbon in these converted soils declined later. The highest rates of carbon decrease were measured between 1.5 and 2 years after forest conversion in the <2 mm soil fraction, from 0.46 kg Cm-2 yr(-1) (in grassland soils) to 0.71 kg C m(-2) yr(-1) (in cropland under no tillage). The carbon pool declined during the third year at rates of 0.41 kg C m(-2) yr(-1) (cropland under disk tillage) to 0.76 kg C m(-2) yr(-1) (grassland soils). Three years after forest conversion, C masses in the top 30 cm of soils for grassland showed similar values than for forest. In comparison, the carbon stock in cropped soils managed under no tillage in direct seeding (without mulch) was significantly 17% and 16% lower than in forest and grassland soils, respectively. None of the studied agricultural practices succeeded in accumulating carbon from the chopped forest biomass.
ISSN: 0167-8809

hal-01032438
https://hal.archives-ouvertes.fr/hal-01032438
DOI : 10.1016/j.agee.2013.11.009

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD Rockefeller University Press
Résumé : International audience
Accumulation of type I collagen fibrils in tumors is associated with an increased risk of metastasis. Invadosomes are F-actin structures able to degrade the extracellular matrix. We previously found that collagen I fibrils induced the formation of peculiar linear invadosomes in an unexpected integrin-independent manner. Here, we show that Discoidin Domain Receptor 1 (DDR1), a collagen receptor overexpressed in cancer, colocalizes with linear invadosomes in tumor cells and is required for their formation and matrix degradation ability. Unexpectedly, DDR1 kinase activity is not required for invadosome formation or activity, nor is Src tyrosine kinase. We show that the RhoGTPase Cdc42 is activated on collagen in a DDR1-dependent manner. Cdc42 and its specific guanine nucleotide-exchange factor (GEF), Tuba, localize to linear invadosomes, and both are required for linear invadosome formation. Finally, DDR1 depletion blocked cell invasion in a collagen gel. Altogether, our data uncover an important role for DDR1, acting through Tuba and Cdc42, in proteolysis-based cell invasion in a collagen-rich environment.
ISSN: 0021-9525

hal-01118481
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01118481
PUBMED : 25422375
DOI : 10.1083/jcb.201404079
PUBMEDCENTRAL : PMC4242841