Éditeur(s) : HAL CCSD
Résumé : International audience
This article describes a previously unreported mutation at position 210 (C210T) of the mitochondrial large subunit ribosomal RNA (mtLSUrRNA) gene of Pneumocystis jirovecii, which led to a false-negative result of a real-time polymerase chain reaction (PCR) assay. Since the aforementioned real-time PCR assay is widely used in France, a French multicenter study was conducted to estimate the mutation frequency and its potential impact on the routine diagnosis of Pneumocystis pneumonia (PCP). Through analysis of data obtained from eight centers, the mutation frequency was estimated at 0.28%. This low frequency should not call into question the routine use of this PCR assay. Nonetheless, the occurrence of the false-negative PCR result provides arguments for maintaining microscopic techniques combined to PCR assays to achieve PCP diagnosis.
Medical Mycology

hal-01477200
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01477200
DOI : 10.1093/mmy/myw051
PUBMED : 27489302

Éditeur(s) : HAL CCSD
Résumé : International audience
Dissertationes Math

hal-00695355
https://hal.archives-ouvertes.fr/hal-00695355

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Ethnopharmacological relevancePsidium acutangulum Mart. ex DC is a small tree used by the Wayana Amerindians from the Upper-Maroni in French Guiana for the treatment of malaria.Aim of the studyIn a previous study, we highlighted the in vitro antiplasmodial, antioxidant and anti-inflammatory potential of the traditional decoction of P. acutangulum aerial parts. Our goal was then to investigate on the origin of the biological activity of the traditional remedy, and eventually characterize active constituents.Materials and methodsLiquid-liquid extractions were performed on the decoction, and the antiplasmodial activity evaluated against chloroquine-resistant FcB1 ([3H]-hypoxanthine bioassay) and 7G8 (pLDH bioassay) P. falciparum strains, and on a chloroquine sensitive NF54 ([3H]-hypoxanthine bioassay) P. falciparum strain. The ethyl acetate fraction (D) was active and underwent bioguided fractionation. All the isolated compounds were tested on P. falciparum FcB1 strain. In vitro anti-inflammatory activity (IL-1β, IL-6, IL-8, TNFα) of the ethyl acetate fraction and of an anti-Plasmodium active compound, was concurrently assessed on LPS-stimulated human PBMC and NO secretion inhibition was measured on LPS stimulated RAW murine macrophages. Cytotoxicity of the fractions and pure compounds was measured on VERO cells, L6 mammalian cells, PBMCs, and RAW cells.ResultsFractionation of the ethyl acetate soluble fraction (IC50 ranging from 3.4 to <1 µg/mL depending on the parasite strain) led to the isolation of six pure compounds: catechin and five glycosylated quercetin derivatives. These compounds have never been isolated from this plant species. Two of these compounds (wayanin and guaijaverin) were found to be moderately active against P. falciparum FcB1 in vitro (IC50 5.5 and 6.9 µM respectively). We proposed the name wayanin during public meetings organized in June 2015 in the Upper-Maroni villages, in homage to the medicinal knowledge of the Wayana population. At 50 µg/mL, the ethyl acetate fraction (D) significantly inhibited IL-1β secretion (−46%) and NO production (−21%), as previously observed for the decoction. The effects of D and guiajaverin (4) on the secretion of other cytokines or NO production were not significant.ConclusionsThe confirmed antiplasmodial activity of the ethyl acetate soluble fraction of the decoction and of the isolated compounds support the previous results obtained on the P. acutangulum decoction. The antiplasmodial activity might be due to a mixture of moderately active non-toxic flavonoids. The anti-inflammatory activities were less marked for ethyl acetate fraction (D) than for the decoction.
ISSN: 0378-8741

hal-01313006
http://hal.upmc.fr/hal-01313006
http://hal.upmc.fr/hal-01313006/document
http://hal.upmc.fr/hal-01313006/file/Houel_Wayanin_and.pdf
DOI : 10.1016/j.jep.2016.04.053

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
We provide a first-order necessary and sufficient condition for optimality of lower semicontinuous functions on Banach spaces using the concept of subdifferential. From the sufficient condition we derive that any subdifferential operator is monotone absorbing, hence maximal monotone when the function is convex.
ISSN: 0925-5001

hal-00761658
https://hal.archives-ouvertes.fr/hal-00761658
ARXIV : 1212.0532
DOI : 10.1007/s10898-013-0078-6

Éditeur(s) : HAL CCSD
Résumé : International audience
We show that the properties of dense subdifferentiability and of trustworthiness are equivalent for any subdifferential satisfying a small set of natural axioms. The proof relies on a remarkable property of the subdifferential of the inf-convolution of two (non necessarily convex) functions. We also show the equivalence of the dense subdifferentiability property with other basic properties of subdifferentials such as a weak∗ Lipschitz Separation property, a strong Compact Separation property and a Minimal property for the analytic approximate subdifferential of Ioffe.
Serdica. Mathematical Journal

hal-00694603
https://hal.archives-ouvertes.fr/hal-00694603

Éditeur(s) : HAL CCSD
Résumé : International audience
J. Convex Anal.

hal-00695362
https://hal.archives-ouvertes.fr/hal-00695362

Éditeur(s) : HAL CCSD Taylor & Francis
Résumé : International audience
We provide an inequality relating the radial directional derivative and the subdifferential of proper lower semicontinuous functions, which extends the known formula for convex functions. We show that this property is equivalent to other subdifferential properties of Banach spaces, such as controlled dense subdifferentiability, optimality criterion, mean value inequality and separation principles. As an application, we obtain a first-order sufficient condition for optimality, which extends the known condition for differentiable functions in finite-dimensional spaces and which amounts to the maximal monotonicity of the subdifferential for convex lower semicontinuous functions. Finally, we establish a formula describing the subdifferential of the sum of a convex lower semicontinuous function with a convex inf-compact function in terms of the sum of their approximate epsilon-subdifferentials. Such a formula directly leads to the known formula relating the directional derivative of a convex lower semicontinuous function to its approximate epsilon-subdifferential.
ISSN: 0233-1934

hal-00694601
https://hal.archives-ouvertes.fr/hal-00694601
DOI : 10.1080/02331934.2011.645034

Éditeur(s) : HAL CCSD Taylor & Francis
Résumé : International audience
ISSN: 0233-1934

hal-00695363
https://hal.archives-ouvertes.fr/hal-00695363

Éditeur(s) : HAL CCSD Springer
Résumé : International audience
It is known that a locally Lipschitz function f is approximately convex if, and only if, its Clarke subdifferential ∂C f is a submonotone operator. The main object of this work is to extend the above characterization to the class of lower semicontinuous functions. To this end, we establish a new approximate mean value inequality involving three points. We also show that an analogue of the Rockafellar maximal monotonicity theorem holds for this class of functions and we discuss the case of arbitrary subdifferentials
Mathematical Programming B

hal-00694605
https://hal.archives-ouvertes.fr/hal-00694605

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490