Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Background: The impact of hepatitis B virus (HBV) genomic variability on the measurement of HBsAg level has been poorly evaluated. Objective: This study was designed to compare the performance of all the available assays measuring HBsAg level in this setting. Study design: A large selection of wild type HBV genotypes (n = 184) and HBsAg strains harboring mutations in the S gene (n = 81) from clinical samples was studied with three HBsAg quantification assays: Architect HBsAg (Abbott), LiaisonXL Murex HBsAg Quant (DiaSorin) and the Elecsys HBsAgII (Roche). Results: The overall percentage of positive results was 99.2% for Abbott, 98.9% for DiaSorin and 98.1% for Roche. Abbott and Roche assays provided an excellent concordance in HBsAg quantification (global mean bias of -0.006 logIU/mL). By contrast, DiaSorin underestimated HBsAg level with values 0.112 logIU/ml and 0.103 logIU/ml lower than Abbott and Roche, respectively. By contrast, DiaSorin slightly over quantified gtC(2.5% over the expected value) while Abbott provided values 6.2% lower than expected and 16.2% lower than what observed for the other genotypes. HBsAg quantitative assays were influenced by HBs protein substitutions irrespective to the genotype but no specific protein pattern that would particularly impair the quantification by one technique has been identified. However, Roche seemed to be particularly impacted by substitutions at 145 residue: 75% of under quantified samples carried a substituted 145 residue. Conclusion: This head-to-head comparison indicates a good correlation between all current systems used to quantify HBsAg but clearly shows an influence of both the genotype and the presence of "a" determinant variants in the absolute quantification of HBsAg. While these discrepancies may not translate into major clinical consequence, they may explain an absence of detection of weak concentration of HBsAg on some systems. (C) 2017 Elsevier B.V. All rights reserved.
ISSN: 1386-6532

hal-01588395
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01588395
DOI : 10.1016/j.jcv.2017.02.001
PUBMED : 28189936

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The contourite depositional system (CDS) along the southwestern Iberian Margin (SIM), within the Gulf of Cadiz and offshore areas of western Portugal bear the unmistakable signal of Mediterranean Outflow Water (MOW) exiting the Strait of Gibraltar. This locality records key information concerning the effects of tectonic activity on margin sedimentation, the effects of MOW dynamics on Atlantic circulation, and how these factors may have influenced global climate. Over the last four decades, numerous studies have been conducted on the late Miocene, Pliocene and Quaternary sedimentary stacking pattern of Neogene basins along the SIM for both academic and resources exploration purposes. However, understanding of the region rests primarily on basic seismic stratigraphy calibrated with limited data from only a few exploration wells. The Integrated Ocean Drilling Program (IODP) Expedition 339 recently drilled five sites in the Gulf of Cadiz and two sites on the western Iberian margin. The integration of core and borehole data with other geophysical databases leads us to propose a new stratigraphic framework. Interpretation of IODP Exp. 339 data along with that from industry sources and onshore outcrop analysis helps refine our understanding of the SIM's sedimentary evolution.We identify significant changes in sedimentation style and dominant sedimentary processes, coupled with widespread depositional hiatuses along the SIM within the Cadiz, Sanlucar, Doñana, Algarve and Alentejo basins. Following the 4.5 Ma cessation of a previous phase of tectonic activity related to the Miocene–Pliocene boundary, tectonics continued to influence margin development, downslope sediment transport and CDS evolution. Sedimentary features indicate tectonic pulses of about 0.8–0.9 Ma duration with a pronounced overprint of ~ 2–2.5 Ma cycles. These more protracted cycles relate to the westward rollback of subducted lithosphere at the convergent Africa-Eurasia plate boundary as its previous NW–SE compressional regime shifted to a WNW–ESE direction. Two major compressional events affecting to the Neogene basins at 3.2–3 Ma and 2–2.3 Ma help constrain the three main stages of CDS evolution. The stages include: 1) the initial-drift stage (5.33–3.2 Ma) with a weak MOW, 2) a transitional-drift stage (3.2–2 Ma) and 3) a growth-drift stage (2 Ma-present time) with enhanced MOW circulation into the Atlantic and associated contourite development due to greater bottom-current velocity. Two minor Pleistocene discontinuities at 0.7–0.9 Ma and 0.3–0.6 Ma record the effects of renewed tectonic activity on basin evolution, appearing most prominently in the Doñana basin. Several discontinuities bounding major and minor units appear on seismic profiles. Quaternary records offer the clearest example of this, with major units of about 0.8–0.9 Ma and sub-units of 0.4–0.5 Ma. Sedimentation is controlled by a combination of tectonics, sediment supply, sea-level and climate. This research identifies time scales of tectonic controls on deep-marine sedimentation, specifically over periods of 2.5–> 0.4 Ma. Shorter-term climatic (orbital) mechanisms control sedimentation at time scales of ≤ 0.4 Ma. The role of bottom water circulation and associated processes in shaping the seafloor and controlling the sedimentary stacking pattern on continental margins has to be seriously reconsidered in future multidisciplinary studies. This is not only because of the common occurrence of sandy contourite deposits in deep water setting and their economic interest for hydrocarbon exploration, but principally because they archive the heartbeat of the interior Earth and therefore have important sedimentary and paleoceanographic implications.
ISSN: 0025-3227

hal-01356166
https://hal.archives-ouvertes.fr/hal-01356166
DOI : 10.1016/j.margeo.2015.09.013

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The base of the Faro contouritic drift (IODP Site U1386) on the northern margin of the Gulf of Cádiz (Algarve margin) is characterized by two sequences of frequent gravity deposits with different ages and compositions. Among these gravity deposits, several relatively thick debrites (up to 12 m) have been observed and studied in detail.Sedimentological analyses have been performed and because of non-turbulent behavior of debris flows, detailed micropaleontological studies could be realized. Planktonic foraminifera thus allowed establishing a detailed biostratigraphy of these deposits. Benthic foraminifer and ostracod assemblages were used to evaluate the origin of the sediment composing these debris flows and estimate their run-out distance.These debrites are dated from Early Pliocene and early Pleistocene, and were deposited in a mesobathyal environment. They comprise silty mud clasts and matrixes with sand content up to 34%. The Early Pliocene debrites are bioclast-rich whereas the Early Pleistocene debrite is enriched in terrigenous particles. The data indicates that these debrites were triggered on the continental shelf and traveled less than 100 km, eroding the seafloor all along their path for the Early Pliocene debrites and only the first part of their path for the early Pleistocene debrite. Matrixes originate from failure areas whereas eroded sediments along the flow pathway are incorporated into the flow as clasts.High abundance of shelf fauna during the Early Pliocene and great supply of terrigenous particles from rivers during the early Pleistocene in the south-western Iberian margin have favored gravity flows from the continental shelf to the slope. The contouritic paleo-moat of the Faro drift has been a determining channeling feature for gravity flows along the Algarve margin during the early Pleistocene, testifying of the strong interaction between MOW circulation and down-slope processes. Tectonic and diapiric activities were significant during Early Pliocene and early Pleistocene on the Algarve margin and could have been triggering parameters of failures related to these debris flows.
ISSN: 0025-3227

hal-01356172
https://hal.archives-ouvertes.fr/hal-01356172
DOI : 10.1016/j.margeo.2015.08.018