Manioc : Portail | resultats 1 - 20 pour la recherche: Hansen, H.

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

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Le livre d'or du négociant. Tome 1
Auteur(s) : Potel, Maurice (1866-19..)
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Cuba por fuera (apuntes del natural)
Auteur(s) : Gallego Garcia, Tesifonte
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La France équinoxiale. [Tome second], Voyage à travers les Guyanes et l'Amazonie
Auteur(s) : Coudreau, Henri Anatole (1859-1899)
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Surinaamsche almanak voor het jaar 1902
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Bagne
Auteur(s) : Maroger, Mireille (1905-1937)
Auteurs secondaires : Chanel, Charles-Jean (1882-1943) Rousseau, Louis
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Geochemistry of a long in-situ section of intrusive slow-spread oceanic lithosphere: Results from IODP Site U1309 (Atlantis Massif, 30 degrees N Mid-Atlantic-Ridge)
Auteur(s) : Godard, Marguerite Awaji, S. Hansen, H. Hellebrand, E. Brunelli, D. Johnson, K. Yamasaki, T. Maeda, J.
Auteurs secondaires : Géosciences Montpellier ; Université des Antilles et de la Guyane (UAG) - Institut national des sciences de l'Univers (INSU - CNRS) - Université de Montpellier (UM) - Centre National de la Recherche Scientifique (CNRS) Department of Geosystem Engineering, University of Tokyo ; Université du Québec University of Bergen, Department of Earth Science ; Université du Québec University of Hawaii, Dept. of Geology and Geophysics, Honolulu ; Université du Québec Dipartimento di Scienze della Terra, Università di Modena ; Université du Québec Univ Hyogo, Dept Mat Sci & Engn, ; Univ Hyogo Institute of Geosciences, University of Jena ; Université du Québec West Coast & Polar Regions Undersea Research Center, University of Alaska, Fairbanks ; Université du Québec

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
IODP Site U1309 was drilled at Atlantis Massif, an oceanic core complex, at 30 degrees N on the Mid-Atlantic Ridge (MAR). We present the results of a bulk rock geochemical study (major and trace elements) carried out on 228 samples representative of the different lithologies sampled at this location. Over 96% of Hole U1309D is made up of gabbroic rocks. Diabases and basalts cross-cut the upper part of the section; they have depleted MORB compositions similar to basalts sampled at MAR 30 degrees N. Relics of mantle were recovered at shallow depth. Mantle peridotites show petrographic and geochemical evidence of extensive melt-rock interactions. Gabbroic rocks comprise: olivine-rich troctolites (>70% modal olivine) and troctolites having high Mg# (82-89), high Ni (up to 2300 ppm) and depleted trace element compositions (Yb 0.06-0.8 ppm); olivine gabbros and gabbros (including gabbronorites) with Mg# of 60-86 and low trace element contents (Yb 0.125-2.5 ppm); and oxide gabbros and leucocratic dykes with low Mg# (<50), low Ni (similar to 65 ppm) and high trace element contents (Yb up to 26 ppm). Troctolites and gabbros are amongst the most primitive and depleted oceanic gabbroic rocks. The main geochemical characteristics of Site U1309 gabbroic rocks are consistent with a formation as a cumulate sequence after a common parental MORB melt, although (lack of systematic) downhole variations indicate that the gabbroic series were built by multiple magma injections. In detail, textural and geochemical variations in olivine-rich troctolites and gabbronorites suggest chemical interaction (assimilation?) between the parental melt and the intruded lithosphere. Site U1309 gabbroic rocks do not represent the complementary magmatic product of 30 degrees N volcanics, although they sample the same mantle source. The bulk trace element composition of Site U1309 gabbroic rocks is similar to primitive MORB melt compositions; this implies that there was no large scale removal of melts from this gabbro section. The occurrence of such a large magmatic sequence implies that a high magmatic activity is associated with the formation of Atlantis Massif. Our results suggest that almost all melts feeding this magmatic system stays trapped into the intruded lithosphere.
ISSN: 0012-821X

hal-00413562
https://hal.archives-ouvertes.fr/hal-00413562
DOI : 10.1016/j.epsl.2008.12.034

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Annuaire de la vie martiniquaise : 1936
Auteur(s) : Sylvestre, Emile (1...-19.. ; fonctionnaire) Lotaut, Eustache
Auteurs secondaires : Eboué, Félix (1884-1944)
Année de publication :

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The PLATO 2.0 mission
Auteur(s) : Rauer, H. Catala, C. Aerts, C. Appourchaux, T. Benz, W. Brandeker, A. Christensen-Dalsgaard, J. Deleuil, M.
Auteurs secondaires : DLR Institut für Planetenforschung ; Deutsches Zentrum für Luft- und Raumfahrt [Berlin] (DLR) Instituut voor Sterrenkunde ; Katholieke Universiteit Leuven (KU Leuven) Institut d'astrophysique spatiale (IAS) ; Université Paris-Sud - Paris 11 (UP11) - Institut national des sciences de l'Univers (INSU - CNRS) - Centre National de la Recherche Scientifique (CNRS) Physikalisches Institut [Bern] ; Universität Bern [Bern] Stockholm University Stellar Astrophysics Centre (DASC) Laboratoire d'études spatiales et d'instrumentation en astrophysique (LESIA) ; Université Pierre et Marie Curie - Paris 6 (UPMC) - Institut national des sciences de l'Univers (INSU - CNRS) - Observatoire de Paris - Université Paris Diderot - Paris 7 (UPD7) - Centre National de la Recherche Scientifique (CNRS) Department of Palaeontology ; National Institutes of Health (NIH) - The Natural History Museum (NHM)

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