Éditeur(s) : HAL CCSD
Résumé : International audience
Introduction: Type 1 diabetes mellitus, a T cell-mediated autoimmune disease, is a complex process that results from the loss of tolerance to insulin and other beta-cell-specific antigens. Diabetes is associated with an increased risk of death from infectious disease. Hyperglycaemia, oxidative stress and inflammation have been identified as the main factor contributing to the development of diseases associated with diabetes mellitus. However, experimental evidence indicates individual susceptibility to develop complications of diabetes. Various genetic and environmental factors have been studied so far, but precise causation has yet to be established. Numerous studies in rodents and human subjects have been performed in order to elucidate the role of B and T cells, which determine the risk of development and progression of diabetes. Moreover, the therapy management of diabetes includes nutritional recommendations and exercise training. The effects of exercise training on glycemia, oxidative stress, inflammation and immune response are well known and favorable to limit the development of diabetes and its complications. More interestingly, ?-galacto-oligosaccharides (?-GOS) are prebiotics that selectively alter the composition of gut microbiota and exhibit strong antioxidant, anti- inflammatory and insulin-sensitive effects in vitro (Efstathiou and Fathi 2010) and in vivo (Boucher J et al. 2003). Thus, ? -GOS is a potential functional food for the treatment of diabetes. But, less is known about the effect of training and ? –GOS on immune response in diabetes. In this context, the aim of this work was to study the immune response in a streptozotocin-induced type 1 diabetes with two therapeutic means: training and ?-galacto-oligosaccharides (?-GOS) alone or combined. Methods: Forty-two male Wistar rats were divided into control placebo, streptozotocin-diabetic rat (STZ) groups supplemented or not with ?-GOS (20 mg/kg/day) for 8 weeks and/or following endurance training. The training program was individualized and consisted in a 1h/day, 5days/week running during 8 weeks on a motor-driven treadmill. At the end of the protocol, blood was obtained by venous puncture into heparin-containing tubes for further analyses (glucose, insulin, fructosamine and immune responses) Results: Relative to the control group, STZ rats exhibited hyperglycemia, insulin deficiency and a trend to higher plasma fructosamine levels, a marker of glycated proteins. They also exhibited a decrease in T and B cells and an increase in natural killers (NK). ?-GOS treatment reduced plasma fructosamine levels and increased B cells compared to STZ rats. Training induced a decrease in plasma glucose, T cells and an increase in B cells in STZ rats. The combined effect of ?-GOS and training didn’t potentiate the immune response on B and T cells but inhibited the effects of training on NK. Discussion: Our results suggest that training and ?-GOS alter positively plasma glucose and/or fructosamine and the immune response. But the effects of combined treatments only concern limited parameters. ? -GOS effects could be partly attributable to its immunomodulating activity. Keywords: ?-GOS, training, immune cells, diabetes. References Efstathiou, T. and D. Fathi. Sojasun Technologies. 2010 Boucher J et al. J Physiol Biochem. 59(3):169-73, 2003
18th Annual Congress of the European College of Sport Sciences, Barcelone, Spain, 26-29 juin.
Barcelone, Spain

hal-01159378
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01159378

Éditeur(s) : HAL CCSD
Résumé : Anopheles darlingi, one of the main malaria vectors in the Neotropics, is widely distributed in French Guiana, where malaria remains a major public-health problem. Elucidation of the relationships between the population dynamics of An. darlingi and local environmental factors would appear to be an essential factor in the epidemiology of human malaria in French Guiana and the design of effective vector-control strategies. In a recent investigation, longitudinal entomological surveys were carried out for 2-4 years in one village in each of three distinct endemic areas of French Guiana. Anopheles darlingi was always the anopheline mosquito that was most frequently caught on human bait, although its relative abundance (as a proportion of all the anophelines collected) and human biting rate (in bites/person-year) differed with the study site. Seasonality in the abundance of human-landing An. darlingi (with peaks at the end of the rainy season) was observed in only two of the three study sites. Just three An. darlingi were found positive for Plasmodium (either P. falciparum or P. vivax) circumsporozoite protein, giving entomological inoculation rates of 0*0-8*7 infectious bites/person-year. Curiously, no infected An. darlingi were collected in the village with the highest incidence of human malaria. Relationships between malaria incidence, An. darlingi densities, rainfall and water levels in the nearest rivers were found to be variable and apparently dependent on land-cover specificities that reflected the diversity and availability of habitats suitable for the development and reproduction of An. darlingi.
Annals of Tropical Medicine and Parasitology / Annals of Tropical Medicine & Parasitology

pasteur-00583507
https://hal-riip.archives-ouvertes.fr/pasteur-00583507
DOI : 10.1179/136485911X12899838683322
PUBMED : 21396247

Éditeur(s) : HAL CCSD EDP Sciences
Résumé : No matter which production system is considered (5% milk farms, 95% meatfarms), the Salers breed of cattle carries a strong image. Our work aims at showing that the development of the main territory in which the breed is found may rely on specific and identified products afforded by the breed. Multidisciplinary research (animal science, economics, food quality) and different methods (surveys, follow-up, and experimentation) were implemented. The experiments focused on the control of various types of production (milk, carcasses) and on a detailed description of the products (meat, cheese), given the attributes and the special behaviour of the animals. A before-weaning feeding approach favouring milk and forage later proves more efficient for finishing the young Salers male (15 months). The meat from these young bulls is clear, classified as mid-range quality, and can be sold in supermarkets. Parallel observations were made on farms and specific protein markers of juiciness were identified. Milk from Salers cows differs from that of Holstein especially in the nature of the fatty acids. Calf suckling before milking reinforces the differences. Cheeses made with raw milk from the breed differ in their textures and flavours. The cow must however be milked with her calf and the level of labour required impedes economic valorisation of the breed. Consumer surveys have confirmed the strong image of the breed that has a good potential for commercial development. Willingness to pay for identified products, however, is stronger for cheese (up to 30%) than for meat (up to 10%). Recognized qualities of products identified as issued from Salers cattle and originality in production systems can bring significant gains to various local actors, which can be considered as territorial rents.
La race bovine Salers bénéficie d’une image forte et reconnue, indépendante du système de production, laitier pour 5 % des élevages, ou allaitant pour 95 %. Pour autant le développement du « berceau de la race » peut-il s’appuyer sur les combinaisons de productions spécifiques et identifiées qu’autorise cette race ? Des recherches multidisciplinaires (zootechnie, économie, qualité des aliments) et des méthodes d’investigation variées (enquêtes, suivis, expérimentation) ont été mises en œuvre pour répondre à cette question. Les expérimentations ont porté sur la maîtrise des itinéraires de production (lait, carcasses) et la connaissance fine des produits (fromages, viandes), compte tenu des aptitudes et du comportement de la race. Une conduite alimentaire privilégiant le lait et les fourrages avant sevrage est ainsi plus efficace pour la production d’un jeune bovin Salers (15 mois). La viande de ces taurillons est claire, classée en gamme intermédiaire, mais peut se valoriser en grande et moyenne distribution. Le lait des vaches Salers diffère de celui des vaches Holstein surtout par la nature de ses acides gras. La tétée d’amorçage du veau spécifique de la race et difficile à supprimer, renforce les différences de composition. Les fromages fabriqués avec ce lait traité à l’état cru diffèrent par leurs textures et arômes. Une filière laitière pérenne pourrait bien valoriser ces différences mais la pénibilité de la traite faite obligatoirement en présence du veau reste un obstacle. Des enquêtes auprès de consommateurs ont confirmé la forte image de la race vectrice de valorisation économique. Leur consentement à payer pour des produits identifiés est cependant plus fort pour le fromage (jusqu’à 30 %) que pour la viande (jusqu’à 10 %). Les qualités reconnues des produits identifiés comme issus de vaches Salers et l’originalité des systèmes de production peuvent apporter des plus-values significatives aux divers acteurs locaux, ce qui peut correspondre à une forme de rente territoriale.
ISSN: 1166-7699

hal-00992549
https://hal.archives-ouvertes.fr/hal-00992549
DOI : 10.1684/agr.2014.0687
PRODINRA : 259206