Manioc : Portail | resultats 1 - 20 pour la recherche: Indel

La France équinoxiale. [Tome second], Voyage à travers les Guyanes et l'Amazonie
Auteur(s) : Coudreau, Henri Anatole (1859-1899)
Année de publication :

| Plus

Genome sequencing of the Trichoderma reesei QM9136 mutant identifies a truncation of the transcriptional regulator XYR1 as the cause for its cellulase-negative phenotype
Auteur(s) : Lichius, Alexander Bidard, Frédérique Buchholz, Franziska Le Crom, Stéphane Martin, Joel Schackwitz, Wendy Austerlitz, Tina Grigoriev, Igor V
Auteurs secondaires : Research division biotechnology and microbiology ; Institute of chemical engineering, Technische Univeritt Wien IFP Energies nouvelles (IFPEN) Analyse des Données à Haut Débit en Génomique (ADHDG) ; Systématique, adaptation, évolution (SAE) ; Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Centre National de la Recherche Scientifique (CNRS) - Evolution Paris Seine ; Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Centre National de la Recherche Scientifique (CNRS) - Université des Antilles et de la Guyane (UAG) - Université Pierre et Marie Curie - Paris 6 (UPMC) - Université Nice Sophia Antipolis (UNS) ; Université Côte d'Azur (UCA) - Université Côte d'Azur (UCA) - Université des Antilles et de la Guyane (UAG) US Department of energy ; Joint genome institute, Walnut Creek William R. Wiley Environmental Molecular Sciences Laboratory (EMSL) ; Pacific Northwest National Laboratory (PNNL)

Éditeur(s) : HAL CCSD BioMed Central
Résumé : International audience
Background : Trichoderma reesei is the main industrial source of cellulases and hemicellulases required for the hydrolysis of biomass to simple sugars, which can then be used in the production of biofuels and biorefineries. The highly productive strains in use today were generated by classical mutagenesis. As byproducts of this procedure, mutants were generated that turned out to be unable to produce cellulases. In order to identify the mutations responsible for this inability, we sequenced the genome of one of these strains, QM9136, and compared it to that of its progenitor T. reesei QM6a.Results : In QM9136, we detected a surprisingly low number of mutagenic events in the promoter and coding regions of genes, i.e. only eight indels and six single nucleotide variants. One of these indels led to a frame-shift in the Zn2Cys6 transcription factor XYR1, the general regulator of cellulase and xylanase expression, and resulted in its C-terminal truncation by 140 amino acids. Retransformation of strain QM9136 with the wild-type xyr1 allele fully recovered the ability to produce cellulases, and is thus the reason for the cellulase-negative phenotype. Introduction of an engineered xyr1 allele containing the truncating point mutation into the moderate producer T. reesei QM9414 rendered this strain also cellulase-negative. The correspondingly truncated XYR1 protein was still able to enter the nucleus, but failed to be expressed over the basal constitutive level.Conclusion : The missing 140 C-terminal amino acids of XYR1 are therefore responsible for its previously observed auto-regulation which is essential for cellulases to be expressed. Our data present a working example of the use of genome sequencing leading to a functional explanation of the QM9136 cellulase-negative phenotype.
ISSN: 1471-2164

Droits : http://creativecommons.org/licenses/by/
hal-01149671
http://hal.upmc.fr/hal-01149671
http://hal.upmc.fr/hal-01149671/document
http://hal.upmc.fr/hal-01149671/file/Lichius_2015_Genome_sequencing.pdf
DOI : 10.1186/s12864-015-1526-0

| Plus

Complete story of the Martinique and St. Vincent horrors
Auteur(s) : Garesche, William A. (1852-1905)
Auteurs secondaires : Demessimy, Vincent (18..-1)
Année de publication :

| Plus

Always look on both sides: phylogenetic information conveyed by simple sequence repeat allele sequences
Auteur(s) : Barthe, Stephanie Gugerli, Felix Barkley, Noelle A. Maggia, Laurent Cardi, Celine Scotti, Ivan
Auteurs secondaires : Ecologie des forêts de Guyane (ECOFOG) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) - Institut National de la Recherche Agronomique (INRA) - Université des Antilles et de la Guyane (UAG) - AgroParisTech - Centre National de la Recherche Scientifique (CNRS) Biodivers & Conservat Biol Res Unit ; Swiss Federal Institute for Forest, Snow and Landscape Research WSL Plant Genet Resources Conservat Unit ; United States Department of Agriculture Unite Mixte Rech Ameliorat Genet & Adaptat Plante ; Institut Agronomique néo-Calédonien Unite Mixte Rech Ameliorat Genet & Adaptat Plante ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement PO-FEDER Guyane "ENERGIRAVI"; European Social Fund

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : Simple sequence repeat (SSR) markers are widely used tools for inferences about genetic diversity, phylogeography and spatial genetic structure. Their applications assume that variation among alleles is essentially caused by an expansion or contraction of the number of repeats and that, accessorily, mutations in the target sequences follow the stepwise mutation model (SMM). Generally speaking, PCR amplicon sizes are used as direct indicators of the number of SSR repeats composing an allele with the data analysis either ignoring the extent of allele size differences or assuming that there is a direct correlation between differences in amplicon size and evolutionary distance. However, without precisely knowing the kind and distribution of polymorphism within an allele (SSR and the associated flanking region (FR) sequences), it is hard to say what kind of evolutionary message is conveyed by such a synthetic descriptor of polymorphism as DNA amplicon size. In this study, we sequenced several SSR alleles in multiple populations of three divergent tree genera and disentangled the types of polymorphisms contained in each portion of the DNA amplicon containing an SSR. The patterns of diversity provided by amplicon size variation, SSR variation itself, insertions/deletions (indels), and single nucleotide polymorphisms (SNPs) observed in the FRs were compared. Amplicon size variation largely reflected SSR repeat number. The amount of variation was as large in FRs as in the SSR itself. The former contributed significantly to the phylogenetic information and sometimes was the main source of differentiation among individuals and populations contained by FR and SSR regions of SSR markers. The presence of mutations occurring at different rates within a marker's sequence offers the opportunity to analyse evolutionary events occurring on various timescales, but at the same time calls for caution in the interpretation of SSR marker data when the distribution of within-locus polymorphism is not known.
ISSN: 1932-6203

hal-01190764
https://hal.archives-ouvertes.fr/hal-01190764
PRODINRA : 214530
PUBMED : 22808236
DOI : 10.1371/journal.pone.0040699

| Plus

The colony of British Guyana and its labouring population
Auteur(s) : Bronkhurst, Rév. H.V.P.
Année de publication :

| Plus

Notice sur la Guyane
Auteur(s) : Bassières, Gustave Eugène (1871-1931)
Auteurs secondaires : Ursleur, Henri Louis Hippolyte (1857-1917)
Année de publication :

| Plus

A natural history of Nevis, and the rest of the English Leeward Charibee Islands in America : with many other observations on nature and art : particularly, an introduction to the art of decyphering
Auteur(s) : Smith, William (17..-1... ; révérend)
Année de publication :

| Plus

Manuel des cultures tropicales. Tome II, 1ère partie, Culture du caféier : semis, plantation, taille, cueillette, dépulpation, décorticage, expédition, commerce, espèces et races
Auteur(s) : Raoul, Edouard François Armand (1845-1898)
Auteurs secondaires : Darolles, Jean-Bertrand-Henry-Edouard (1843-1916)
Année de publication :

| Plus

13.904 : roman d'un forçat
Auteur(s) : Merlet, Jean-François-Louis (1878-1942)
Année de publication :

| Plus

L'enfer du bagne
Auteur(s) : Murri, Tullio (1874-1930)
Auteurs secondaires : Hervo, Georges Dunan Renée (1892-1936)
Année de publication :

| Plus

La France équinoxiale. [Tome second], Voyage à travers les Guyanes et l'Amazonie
Auteur(s) : Coudreau, Henri Anatole (1859-1899)
Année de publication :

| Plus

Antigua and the Antiguans: a full account of the colony and its inhabitants from the time of the Caribs to the present day, interspersed with anecdotes and legends : also, an impartial view of slavery and the free labour systems, the statistics of the island, and biographical notices of the principal families, Volume 1
Année de publication :

| Plus

Four years' residence in the West Indies
Auteur(s) : Bayley, Frederic William Naylor (1808-1853)
Auteurs secondaires : Haghe, Charles (1810-1888) Day, William (1797-1845)
Année de publication :

| Plus

The Life and voyages of Christopher Colombus ; together with the voyages of his companions (vol. 2)
Auteur(s) : Irving, Washington (1783-1859)
Année de publication :

| Plus

A voyage to the Demerary : containing a statistical account of the settlements there, and of those on the Essequebo, the Berbice, and others contiguous rivers of Guyana
Auteur(s) : Bolingbroke, Henry (1785-1855)
Année de publication :

| Plus

Voyage pittoresque dans les deux Amériques : résumé général de tous les voyages
Auteur(s) : Orbigny, Alcide d' (1802-1857)
Auteurs secondaires : Sainson
Année de publication :

| Plus

Antigua and the Antiguans: a full account of the colony and its inhabitants from the time of the Caribs to the present day, interspersed with anecdotes and legends : also, an impartial view of slavery and the free labour systems, the statistics of the island, and biographical notices of the principal families, Volume 2
Année de publication :

| Plus

Narrative of a five years' expedition against the revolted negroes of Surinam in Guiana, on the wild coast of South America from the year 1772 to 1777, elucidating the history of that country and describing its productions... with an account of the Indians of Guiana and negroes of Guinea... Tome 1
Auteur(s) : Stedman, John Gabriel (1744-1797)
Année de publication :

| Plus

The magic island
Auteur(s) : Seabrook, William Buehler (1886-1945)
Année de publication :

| Plus

Les colonies et la politique coloniale de la France
Auteur(s) : Duval, Jules (1813-1870)
Année de publication :

| Plus