Éditeur(s) : Caribbean Agricultural Research and Development Institute (CARDI) Caribbean Agricultural Research and Development Institute (CARDI)
Résumé : (Funding) Electronic resources created as part of a prototype UF Institutional Repository and Faculty Papers project by the University of Florida.
Caribbean

29301579

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD
Résumé : 38 pages
Rapport
The Chicxulub impact crater, México, is unique. It is the only known terrestrial impact structure that has been directly linked to a mass extinction event and the only terrestrial impact with a global ejecta layer. Of the three largest impact structures on Earth, Chicxulub is the best preserved. Chicxulub is also the only known terrestrial impact structure with an intact, unequivocal topographic peak ring. Chicxulub’s role in the Cretaceous/Paleogene (K-Pg) mass extinction and its exceptional state of preservation make it an important natural laboratory for the study of both large impact crater formation on Earth and other planets and the effects of large impacts on the Earth’s environment and ecology. Our understanding of the impact process is far from complete, and despite more than 30 years of intense debate, we are still striving to answer the question as to why this impact was so catastrophic.During International Ocean Discovery Program (IODP) Expedition 364, Paleogene sediments and lithologies that make up the Chicxulub peak ring were cored to investigate (1) the nature and formational mechanism of peak rings, (2) how rocks are weakened during large impacts, (3) the nature and extent of post-impact hydrothermal circulation, (4) the deep biosphere and habitability of the peak ring, and (5) the recovery of life in a sterile zone. Other key targets included sampling the transition through a rare midlatitude section that might include Eocene and Paleocene hyperthermals and/or the Paleocene/Eocene Thermal Maximum (PETM); the composition and character of the impact breccias, melt rocks, and peak-ring rocks; the sedimentology and stratigraphy of the Paleocene–Eocene Chicxulub impact basin infill; the chronology of the peak-ring rocks; and any observations from the core that may help us constrain the volume of dust and climatically active gases released into the stratosphere by this impact. Petrophysical property measurements on the core and wireline logs acquired during Expedition 364 will be used to calibrate geophysical models, including seismic reflection and potential field data, and the integration of all the data will calibrate impact crater models for crater formation and environmental effects. The proposed drilling directly contributes to IODP Science Plan goals:Climate and Ocean Change: How resilient is the ocean to chemical perturbations? The Chicxulub impact represents an external forcing event that caused a 75% level mass extinction. The impact basin may also record key hyperthermals within the Paleogene.Biosphere Frontiers: What are the origin, composition, and global significance of subseafloor communities? What are the limits of life in the subseafloor? How sensitive are ecosystems and biodiversity to environmental change? Impact craters can create habitats for subsurface life, and Chicxulub may provide information on potential habitats for life, including extremophiles, on the early Earth and other planetary bodies. Paleontological and geochemical studies at ground zero will document how large impacts affect ecosystems and effects on biodiversity.Earth Connections/Earth in Motion: What are the composition, structure and dynamics of Earth’s upper mantle? What mechanisms control the occurrence of destructive earthquakes, landslides, and tsunami? Mantle uplift in response to impacts provides insight into dynamics that differ between Earth and other rocky planets. Impacts generate earthquakes, landslides, and tsunami, and scales that generally exceed plate tectonic processes yield insight into effects, the geologic record, and potential hazards.IODP Expedition 364 was a Mission Specific Platform expedition to obtain subseabed samples and downhole logging measurements from the sedimentary cover sequence and peak ring of the Chicxulub impact crater. A single borehole was drilled into the Chicxulub impact crater on the Yucatán continental shelf, recovering core from 505.7 to 1334.73 m below seafloor with ~99% core recovery and acquiring downhole logs for the entire depth.
International Ocean Discovery Program

hal-01639520
https://hal.archives-ouvertes.fr/hal-01639520
DOI : 10.14379/iodp.pr.364.2017

Éditeur(s) : HAL CCSD Seismological Society of America
Résumé : With the assistance of the ASTER team (M. Arnold, G. Aumaître, D. Bourlès, and K. Keddadouche)
International audience
The Bulnay earthquake of 23 July 1905 (Mw 8.3–8.5), in north‐central Mongolia, is one of the world’s largest recorded intracontinental earthquakes and one of four great earthquakes that occurred in the region during the twentieth century. The 375 km long surface rupture of the left‐lateral, strike‐slip, N095°E‐trending Bulnay fault associated with this earthquake is remarkable for its pronounced expression across the landscape and for the size of features produced by previous earthquakes. Our field observations suggest that in many areas the width and geometry of the rupture zone is the result of repeated earthquakes; however, in those areas where it is possible to determine that the geomorphic features are the result of the 1905 surface rupture alone, the size of the features produced by this single earthquake are singular in comparison to most other historical strike‐slip surface ruptures worldwide. Along the 80 km stretch, between 97.18° E and 98.33° E, the fault zone is characterized by several meters width and the mean left‐lateral 1905 offset is 8.9±0.6  m with two measured cumulative offsets that are twice the 1905 slip. These observations suggest that the displacement produced during the penultimate event was similar to the 1905 slip. Morphotectonic analyses carried out at three sites along the eastern part of the Bulnay fault allow us to estimate a mean horizontal slip rate of 3.1±1.7  mm/yr over the Late Pleistocene–Holocene period. In parallel, paleoseismological investigations show evidence for two earthquakes prior to the 1905 event, with recurrence intervals of ∼2700–4000  yrs.
ISSN: 0037-1106

hal-01179837
https://hal.archives-ouvertes.fr/hal-01179837
https://hal.archives-ouvertes.fr/hal-01179837v2/document
https://hal.archives-ouvertes.fr/hal-01179837/file/rizza2015.pdf
DOI : 10.1785/0120140119

Éditeur(s) : HAL CCSD American Geophysical Union (AGU)
Résumé : International audience
We studied the patterns, rates and evolution of fluvial terraces and fault system during the building process of an intracontinental transpressional mountain in the Gobi-Altay (Mongolia). By analyzing incisions and offsets of fluvial terraces and alluvial fans, we show that the massif has grown by outward migration of thrust faults through time. On the northern flank, the present bounding thrust fault began its activity ~600 ka ago, while a more internal sub-parallel fault was still active until ~200-100 ka. Vertical offset of an alluvial fan abandoned ~100 ka ago allows an estimate of 0.1 mm/yr Upper Pleistocene - Holocene uplift rate. The morphology of the catchment-piedmont system strongly suggests a periodical formation of the alluvial surfaces, controlled by the climatic pulses, at the beginning of the wet interglacial periods. The abandonment of the alluvial terraces lags by several thousand years the abandonment of the alluvial fans, showing a diachronous incision propagating upstream. The incision rate deduced from the different elevations of straths exceeds of one order of magnitude the rock uplift rate. This excess is mostly due to ongoing drainage network growth at the core of the massif, and incision due to alluvial apron entrenchment near the outlet. This implies that fluvial response is mainly controlled by drainage growth, interaction with piedmont and cyclic climatic variations, rather than by rock uplift.
ISSN: 0278-7407

hal-00323558
https://hal.archives-ouvertes.fr/hal-00323558
https://hal.archives-ouvertes.fr/hal-00323558v2/document
https://hal.archives-ouvertes.fr/hal-00323558/file/Vassallo2007.pdf
DOI : 10.1029/2006TC002081

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : 9 pages
International audience
Potentially hazardous asteroids and comets have hit Earth throughout its history, with catastrophic consequences in the case of the Chicxulub impact. Here we reexamine one of the mechanisms that allow an impact to have a global effect—the release of climate-active gases from sedimentary rocks. We use the SOVA hydrocode and model ejected materials for a sufficient time after impact to quantify the volume of gases that reach high enough altitudes (> 25 km) to have global consequences. We vary impact angle, sediment thickness and porosity, water depth, and shock pressure for devolatilization and present the results in a dimensionless form so that the released gases can be estimated for any impact into a sedimentary target. Using new constraints on the Chicxulub impact angle and target composition, we estimate that 325 ± 130 Gt of sulfur and 425 ± 160 Gt CO2 were ejected and produced severe changes to the global climate.
ISSN: 0094-8276

hal-01639659
https://hal.archives-ouvertes.fr/hal-01639659
DOI : 10.1002/2017GL074879

Éditeur(s) : HAL CCSD American Association for the Advancement of Science
Résumé : The biodiversity-productivity relationship (BPR) is foundational to our understanding of the global extinction crisis and its impacts on ecosystem functioning. Understanding BPR is critical for the accurate valuation and effective conservation of biodiversity. Using ground-sourced data from 777,126 permanent plots, spanning 44 countries and most terrestrial biomes, we reveal a globally consistent positive concave-down BPR, showing that continued biodiversity loss would result in an accelerating decline in forest productivity worldwide. The value of biodiversity in maintaining commercial forest productivity alone-US$166 billion to 490 billion per year according to our estimation-is more than twice what it would cost to implement effective global conservation. This highlights the need for a worldwide reassessment of biodiversity values, forest management strategies, and conservation priorities.
ISSN: 0036-8075

hal-01607784
https://hal.archives-ouvertes.fr/hal-01607784
DOI : 10.1126/science.aaf8957
PRODINRA : 389856

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
A subject of continuing debate is how the Earth's lithospheric portion of the upper mantle has remained the thickest (> 200 km) and oldest (> 3 Gy) beneath cratons and is yet surrounded by a vigorously convecting asthenosphere. It is generally admitted that water is a key parameter in the strength and longevity of cratonic roots, because olivine, the main phase of the lithospheric mantle, becomes stronger if its water content decreases. Expanding upon the work presented in Novella et al. (2015) and Taylor et al. (2016), we report new water contents for additional olivine inclusions in diamonds together with the trace-element composition for all olivine inclusions, as well as for mantle xenoliths from various kimberlite pipes located on the Siberian craton. The olivine diamond inclusions from this study have systematically low-water contents (< 50 ppmw H2O), moderate to high forsterite (e.g., Fo91–94) contents and low Ni, Co, and Zn ppm contents (e.g., < 2848, < 108, and < 47 ppm, respectively). In contrast, olivines from Siberian craton mantle xenoliths have a wide range of water contents (6–323 ppmw H2O) and extend to lower-Fo (91–92), Ni, Co, and Zn-rich compositions, compared to the diamond inclusions. Depleted incompatible trace-element concentrations in olivine (0.1–0.001 × Primitive Mantle) advance our hypothesis for the protogenetic origins for the majority of Siberian diamond inclusions. These observations are consistent with the peridotite xenoliths as representing a part of the cratonic lithosphere that has experienced melt re-fertilization, which has also transported water. The olivine diamond inclusions, on the other hand, preserve “micro-samples” of an initial, dry cratonic lithosphere, mostly resulting from melting events. These inclusions are likely sourced from the initial cratonic mantle lithosphere, which thereby, resisted delamination over time, due to its buoyancy and strength, imparted from melt and water depletion, respectively. And thus, our data provides a major argument that the kimberlite-hosted mantle xenoliths may be more metasomatized than common rocks at the base of the Siberian and other cratonic roots away from kimberlite fields.
ISSN: 0024-4937

hal-01467731
https://hal.archives-ouvertes.fr/hal-01467731
DOI : 10.1016/j.lithos.2016.07.023

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We report new data on the geochemical evolution, metasomatic and deformation processes in the lower layers of cratonic lithospheric mantle based on a detailed study of extraordinarily fresh and large deformed peridotite xenoliths from the Udachnaya kimberlite pipe, Siberia. Based on our T-P estimates, the deformed peridotites are localized in a depth range of 170 to 220 km near the base of cratonic mantle. The degree of deformation is not correlated with the depth and equilibration temperatures of the xenoliths. The deformed peridotites are depleted in magmaphile major elements indicating their residual nature, but are enriched in incompatible trace elements, Fe and K. The deformed peridotites experienced a complex evolution, i.e., they were formed initially as high-degree melt extraction residues and later were subjected to three main stages of metasomatic modification. (1) An old, mostly cryptic metasomatism by melt/fluid of carbonatitic composition, which formed garnets with sinusoidal REE patterns (Smn/Ern > 1). (2) Silicate metasomatism, which led to the most significant changes in mineralogical and chemical composition of the deformed peridotites. (3) Fe and Ti metasomatism just before the entrainment of the deformed rocks into kimberlite magma. Enrichment of the peridotites in large-ion lithophile elements (K, Rb, Ba) is related to the formation of kelyphitic rims around garnet. The nature of the metasomatic agent of silicate metasomatism was evaluated from mass-balance of measured and calculated whole-rock compositions, ratios of highly incompatible elements (D < 0.1) and fractional crystallization modeling. All this evidence indicates that the agent of silicate metasomatism had a composition intermediate between that of kimberlites and HIMU OIB. The metasomatic processes responsible for the formation of deformed peridotites and precipitation of megacryst suites cannot be widespread at the base of the cratonic mantle. Rather, they are local features only existing in the cratonic mantle below kimberlite fields and localized along the metasomatic vein system.
ISSN: 0024-4937

hal-00941010
https://hal.archives-ouvertes.fr/hal-00941010
DOI : 10.1016/j.lithos.2012.11.014

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
ISSN: 0169-555X

hal-01671310
https://hal.inria.fr/hal-01671310
DOI : 10.1016/j.geomorph.2017.11.019