Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
The strike-slip faults of north Tibet accommodate part of the Cenozoic convergence between India and Asia. Along the Karakax valley south of Yecheng and near the Xidatan trough south of Golmud, the active traces of the Altyn-Tagh and Kunlun faults follow narrow belts of metamorphic rocks. The deformation recorded in those mylonites is sinistral strike-slip. Rb/Sr and 40Ar/39Ar ages of deformation from syntectonic fabrics formed at 350–400°C 120 Ma. Argon loss suggests that deformation was associated to a 250–300°C thermal pulse that lasted 5–20 Ma after the onset of movement. Unroofing occurred much later, around 25 Ma when sudden cooling suggests a component of thrusting or more likely normal faulting. The Cretaceous shear may be related to collision between the Qiantang and the Lhasa blocks. The Karakax and Xidatan shear zones may have formed a unique, continuous boundary in the Cretaceous, which was later reused by the Tertiary strike-slip faults, leading to potentially calculable offsets along the Altyn-Tagh fault.
ISSN: 2169-9313

hal-00197562
https://hal.archives-ouvertes.fr/hal-00197562
https://hal.archives-ouvertes.fr/hal-00197562/document
https://hal.archives-ouvertes.fr/hal-00197562/file/Arnaud_JGR000904_def.pdf
DOI : 10.1029/2001JB000904