Éditeur(s) : HAL CCSD Société géologique de France
Résumé : Rock foundations of the Kephren and Kheops pyramids are examined in comparison with other Fourth Dynasty monuments: the Sphinx, Queen Kentkawes' monument and the Abu Rawash monument. This study is based on geological and topographical observations, photomontages and field measurements. The results, which are correlated with those of former studies, demonstrate the existence of natural hills used as substrata in the construction of the two great pyramids. The minimum volume of these hills can be estimated at 12% and 23% respectively of the volumes of the Kephren and Kheops pyramids. The use of worked rock hills appears to be a construction method under the Fourth Dynasty.
EISSN: 1777-5817

hal-00512644
https://hal.archives-ouvertes.fr/hal-00512644

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD
Résumé : 22 pages; 20 figures
Rock foundations of the Kephren and Kheops pyramids are examined in comparison with other Fourth Dynasty monuments: the Sphinx, Queen Kentkawes' mastaba and the Abu Rawash pyramid. This study is based on geological and geomorphological observations, visual observation, and photomontages. Results, correlated with those of former studies, demonstrate the existence of natural hills used as substrata in the construction of the two great pyramids. The minimum volume of these hills can be estimated at 12% and 23% respectively of the volumes of the Kephren and Kheops pyramids. The use of worked rock hills appears to be a characteristic of the construction methods under the Fourth Dynasty.
Le substratum rocheux des pyramides de Kheops et Khephren est étudié en comparaison avec celui d'autres monuments de la quatrième dynastie de l'ancienne Egypte : le Sphinx, le mastaba de la reine Kentkawes et la pyramide d'Abu Rawash. Cette étude est basée sur des observations géologiques et géomorphologiques, l'observation visuelle et des photomontages, ainsi que des mesures réalisées sur le terrain. Les résultats, corrélés avec ceux d'études antérieures, démontrent l'existence de collines naturelles utilisées comme assises pour la construction des deux grandes pyramides. Le volume minimum de ces collines peut être estimé par rapport au volume total à 12% pour Khephren et 23% pour Kheops. L'utilisation de collines rocheuses pour asseoir la construction d'un monument apparaît être caractéristique des méthodes de construction utilisées sous la quatrième dynastie.
https://hal.archives-ouvertes.fr/hal-00319586

hal-00319586
https://hal.archives-ouvertes.fr/hal-00319586
https://hal.archives-ouvertes.fr/hal-00319586/document
https://hal.archives-ouvertes.fr/hal-00319586/file/PyramidsSR.pdf