Éditeur(s) : International Agricultural Development Service International Agricultural Development Service
Caribbean

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD Cerimes
Résumé : L'ensemble des articles est archivé sur ArchiveTémaTice. Les actes sont aussi accessibles sur le site du colloque http://www.cerimes.fr/colloquefrancomexicain
Les textes qui composent ces actes ont été présentés à l'occasion du premier colloque franco-mexicain en sciences de la communication, organisé par la Asociación mexicana en Ciencias de la comunicación (AMIC) et la Société Française des Sciences de l'Information et de la Communication (SFSIC), à Mexico, du 8 au 10 avril 2002.

Les médias classiques: radio, télévision (8 avril 2002)
Introduction . La coopération scientifique bilatérale et l'interculturel dans l'activité de recherche, Bruno Ollivier
La recherche française sur le média et les discours télévisuels: éléments pour un bilan prospectif, Guy Lochard
Parcours de la programmation télévisée au Mexique, Florence Toussaint
El regimen de la television hertziana en Mexico, Claudia I. Garcia Rubio
La question de l'éthos et du genre en communication politique, Simone Bonnafous
L'exposition de la vie privée dans les médias, André Vitalis

Identités, culture, communication (9 avril 2002)
Reconfiguraciones cimunicativas de la socialidad y reencantamientos de la communication identidad, Jesus Martin-Barbero
Identité, culture et communication, Jean François Tetu
Las organizaciones civiles en las radiodifusoras comerciales, Dr. José Cisneros E.
Les SIC et l'altérité: démarches d'une recherche sur la Chine. réflexions épitstémologiques sur les analyses de corpus à base communicationnelles de langue et de culture non-maternelles, Dominique Colomb
Transicion, reforma del estado y medios de comunicacion en Mexico, Dr. Javier Esteinou Madrid
Medios masivos y formacion psicosocial, Enrique Guinsberg

Technologies d'information et de communication ( 10 avril 2002)
Quoi de nouveau dans la recherche française en informatique et communication ? Bernard Miege
Convergencia tecnologica: perspectivas de investigacion en Mexico, Delia Crovi Druetta
Medias, technologies et public: la notion de projet au cœur d'une éventuelle médiation, Claire Noy
La formacion de profesores de educacion superior en el uso de la computadora y la internet. reflexioànes sobre el tema, Luz Maria Garay Cruz
Vers un retour à une lecture non linéaire, Schmidt C. T, Roxin I.(), Mercier D, Hufschmitt B. & Cotten J.-P
NTIC et éducation à distance. Le cas d'Edusat au Mexique face au cas français, Norma Veronica Fernandez Arriaga.
Les sciences de l'éducation et les sciences de la communication en dialogue: à propos des médias et des technologies éducatives, Geneviève Jacquinot-Delaunay
Internet et Intranet dans les organisations "un scénario récurrent", Jean-Antoine Corbalan
De l'université virtuelle au campus numérique : simple effet de traduction ou changement de paradigme, Françoise Thibault
Competencias mediacionales de los sujetos en programas educativos en entornos digitales, Maria Elena Chan Nunez
Le journal quotidien sur le Web. L'identité éditoriale au risque du formatage, Annelise Touboul
Conclusion, Carmen Gomez-Mont
Premier colloque franco-mexicain de sciences de la communication
Mexico, France

Droits : info:eu-repo/semantics/OpenAccess
ISBN : http://www.cerimes.education.fr
edutice-00000362
https://edutice.archives-ouvertes.fr/edutice-00000362
https://edutice.archives-ouvertes.fr/edutice-00000362/document
https://edutice.archives-ouvertes.fr/edutice-00000362/file/actes.pdf

Éditeur(s) : Elsevier
Résumé : We investigated the influence of bioturbation by macrofauna on the vertical distribution of living (stained) benthic foraminifera in marine intertidal sediments. We investigated the links between macrofaunal bioturbation and foraminiferal distribution, by sampling from stations situated on a gradient of perturbation by oyster-farming, which has a major effect on benthic faunal assemblages. Sediment cores were collected on the French Atlantic coast, from three intertidal stations: an oyster farm, an area without oysters but affected by oyster biodeposits, and a control station. Axial tomodensitometry (CT-scan) was used for three-dimensional visualization and two-dimensional analysis of the cores. Biogenic structure volumes were quantified and compared between cores. We collected the macrofauna, living foraminifera, shells and gravel from the cores after scanning, to validate image analysis. We did not investigate differences in the biogenic structure volume between cores. However, biogenic structure volume is not necessarily proportional to the extent of bioturbation in a core, given that many biodiffusive activities cannot be detected on CT-scans. Biodiffusors and larger gallery-diffusors were abundant in macrofaunal assemblage at the control station. By contrast, macrofaunal assemblages consisted principally of downward-conveyors at the two stations affected by oyster farming. At the control station, the vertical distribution of biogenic structures mainly built by the biodiffusor Scorbicularia plana and the large gallery-diffusor Hediste diversicolor was significantly correlated with the vertical profiles of living foraminifera in the sediment, whereas vertical distributions of foraminifera and downward-conveyors were not correlated at the station affected by oyster farming. This relationship was probably responsible for the collection of foraminifera in deep sediment layers (> 6 cm below the sediment surface) at the control station. As previously suggested for other species, oxygen diffusion may occur via the burrows built by S. plana and H. diversicolor, potentially increasing oxygen penetration and providing a favorable microhabitat for foraminifera in terms of oxygen levels. By contrast, the absence of living foraminifera below 6 cm at the stations affected by oyster farming was probably associated with a lack of biodiffusor and large gallery-diffusor bioturbation. Our findings suggest that the effect of macrofaunal bioturbation on the vertical distribution of foraminiferal assemblages in sediments depends on the effects of the macrofauna on bioirrigation and sediment oxidation, as deduced by Eh values, rather than on the biogenic structure volume produced by macrofauna. The loss of bioturbator functional diversity due to oyster farming may thus indirectly affect infaunal communities by suppressing favorable microhabitats produced by bioturbation.
Journal of Experimental Marine Biology and Ecology (0022-0981) (Elsevier), 2009-03 , Vol. 371 , N. 1 , P. 20-33

Droits : 2009 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/2009/publication-6146.pdf
DOI:10.1016/j.jembe.2008.12.012
http://archimer.ifremer.fr/doc/00000/6146/

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The barrier reef tract of New Caledonia is the second largest in the world enclosing lagoons of variable width. The Late Quaternary evolution of these barrier reefs and lagoons is poorly known. A recent high resolution seismic and multibeam bathymetry survey was carried out in the south-western lagoon from shoreline to open ocean across the barrier reef pass systems to perform sequence stratigraphy and to better understand the Quaternary evolution of the lagoon. Two depositional sequences bounded by type-1 erosional surfaces can be identified. The bounding surfaces delineate the bottom of two generations of incised valleys. System tracts comprising a complete succession of regressive, lowstand, transgressive and highstand tracts were observed only in the upper sequence. Chronostratigraphic and sedimentological interpretations of the seismic data are based on correlations with the chronology defined in the nearby cores drilled through the reefs and in the gravity cores previously recovered in the inner lagoon. Interpretation of seismic data is also carried out on the basis of correlation with multibeam bathymetric data. Our results indicate that the history of the New Caledonian lagoon probably does not exceed 200ka marked by two or three brief episodes each of immersion of about ten thousand years. The bathymetric data reveal that during successive low sea level stands, the lagoon was dependent upon erosional fluvial networks. The deep fluvial valleys were connected to the continental slope through the passes. Seismic records reveal that upstream palaeo-channels are not connected to the modern coastal rivers. In contrast, many aggrading deposits, including numerous stacking channels, and extending across the proximal part of the outer lagoon, can be identified. Examination of drainage network morphology suggests a progressive tilt of the outer lagoon. Such evolution reveals that the Nouméa lagoon which is one of the widest in the world is an exceptional modern example of a rimmed shelf providing data to analyze the past counterparts.
ISSN: 0031-0182

insu-00338953
https://hal-insu.archives-ouvertes.fr/insu-00338953
DOI : 10.1016/j.palaeo.2008.08.012

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Grande Terre of New Caledonia is enclosed by one of the longest barrier reefs in the world. For the first time, the fore-reef slopes of this barrier reef have been sampled by dredging, from 40 to 320 m deep, in order to analyze their sedimentological and biological characteristics. The rocks and sediments can be divided into seven sedimentary facies: bindstones dominated by coralline algal crusts, bindstones dominated by foraminiferal crusts, bindstones dominated by bryozoan crusts, coral framestones, bindstones and interstratified packstones rich in skeletal debris, packstones/wackestones and grainstones rich in rock gravels. Radiocarbon dating performed on encrusting organisms (coralline algae and acervulinids) and corals provide ages relatively young. These ages confirm that the encrusting organisms are modern and the corals mainly dated of Holocene are reworked due to the instability of the fore-reef slopes, especially during storms. Because the biological assemblages are distributed according to a bathymetric range depending on light intensity, a model of distribution of modern encrusting calcareous organisms can be proposed from our observations and analyses. From the upper reef slopes to approximately 90 m, thick coralline algal crusts are dominant and distributed in three groups. Group C, the shallowest parts of the fore slopes, is mainly characterized by mastophorids (Hydrolithon reinboldii, H. cf. munitum, Lithoporella melobesoides, Aethesolithon cf. problematicum, Neogoniolithon sp. and undetermined species) and lithophylloids (Lithophyllum sp., L. pustulatum). Group B, composed of lithophylloids (Lithophyllum sp., L. cf. kotschyanum, L. cf. moluccence, L. pustulatum), Mesophyllum sp. and Peyssonnelia sp. occurs from 15 to 40 m. Group A, rich in Mesophyllum sp., M. cf. mesomorphum, Peyssonnelia sp. and Sporolithon sp. is characteristic of deep reef slopes up to 90 m. Below approximately 90 m, when the light intensity decreases, the encrusting foraminifera acervulinids progressively replace the coralline algal crusts. Such a model is particularly useful to interpret and reconstruct the past Quaternary reef environments rich in crusts of coralline algae and/or foraminifera.
ISSN: 0025-3227

hal-00393705
https://hal.archives-ouvertes.fr/hal-00393705
DOI : 10.1016/j.margeo.2007.12.002

Éditeur(s) : HAL CCSD Human Kinetics
Résumé : International audience
In order to study the acclimatization process over 14 days of exposure to tropical climate, 9 triathletes performed 4 outdoor indirect continuous multistage tests in both thermoneutral and tropical conditions. The thermoneutral test (TN, 14 C, 45% rh) was performed before traveling to the tropical area (Martinique, FWI). The tropical tests were performed 2, 8, and 14 days after arrival (32.9 C, 78% rh). During each trial, we measured tympanic temperature, sweat rate, body mass loss, heart rate (HR), and performance. The results showed that 1) the mean tympanic temperature was greater in T2 (P < .001), T8 (P < .01) and T14 (P < .01) than in TN and significantly lower in T14 than in T2 (P < .05); 2) the mean sweat rate was significantly greater (P < .001) in T2, T8 and T14 than in TN and significantly greater (P < .05) in T8 and T14 than in T2; 3) the body mass loss after trials was significantly greater (P < .001) in T2, T8 and T14 than in TN and significantly greater (P < .05) in T8 and T14 than in T2; 4) the mean HR and HR at rest were significantly higher (P < .005) in T2 than in TN, T8, T14 and the mean HR was significantly lower (P < .05) in T14 than in the other trials; and 5) the performance time was significantly lower in T2 (P < 0.02), T8 (P < 0.03) and T14 (P < 0.05) than in TN. We concluded that 14 days of exposure.
ISSN: 1066-7814

hal-00720075
https://hal.univ-antilles.fr/hal-00720075
https://hal.univ-antilles.fr/hal-00720075/document
https://hal.univ-antilles.fr/hal-00720075/file/02.Voltaire_551_.pdf

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
We present a new interpretation of anisotropy of magnetic susceptibility (AMS) fabrics in basaltic lava flows based on the detailed study of magnetic mineralogy and silicate crystallographic fabric of a Quaternary lava flow from the French Massif Central (La Palisse). We consider the model of AMS fabric imbrication between magnetic foliation and flow surface, as initially proposed for dykes. At the two sampling sites, the concordance between the flow direction deduced from the AMS foliation and that deduced from field observations indicates that the imbrication model could apply to the lava flows. However, the flow senses inferred fromAMSare systematically opposed between the two sampling sites suggesting permutations between K1 and K3 AMS axes, a configuration referred to as inverse fabric. Electron backscatter diffraction (EBSD) measurements show strong lattice-preferred orientations (LPO) for plagioclase, especially the (010) plagioclase plane, which tends to be parallel to the flow. Clinopyroxene LPO remains less marked than plagioclase LPO, whereas titanomagnetite does not display a significant LPO. Comparison between magnetic and crystallographic fabrics suggests that the AMS fabric of the lava flow results from the distribution of titanomagnetite grains, which is in turn controlled by the fabric of the silicate framework. Magnetic hysteresis parameters and anisotropy of remanent magnetization (ARM) measurements exclude a significant contribution from single-domain grains, often called upon to explain inverse magnetic fabrics. The origin of the observed inverse magnetic fabric may relate to the dip of the palaeosurface, which is the only remarkable difference between the two sampling sites. AMS appears as a good tool to determine the direction of basaltic lava flows and coupling with local crystallographic fabric data provides a valuable control of relationships between magnetic fabrics and flow and thus contributes to better constrain the AMS signature of lava flows.
ISSN: 0956-540X

hal-00903561
https://hal.archives-ouvertes.fr/hal-00903561
https://hal.archives-ouvertes.fr/hal-00903561/document
https://hal.archives-ouvertes.fr/hal-00903561/file/TB-GJI-193-2-Orig.pdf
DOI : 10.1093/gji/ggs115