Éditeur(s) : Université des Antilles et de la Guyane. Service commun de la documentation Université des Antilles et de la Guyane. Service commun de la documentation
Extrait de : Les rencontres culturelles de la BU 2014-2015. Université des Antilles
Description : Dominique Berthet présente d'abord les intervenants. Manuel Norvat présente ensuite le 20e numéro de la revue Recherche en esthétique en rendant compte de sa propre expérience de lecture. Christelle Lozère tentera enfin de répondre aux deux questions suivantes : "Peut-on parler d'une spécificité de la création insulaire ? En quoi l'île peut-elle être vécue comme un moteur de création artistique ou comme un frein ?
Siècle(s) traité(s) : 21
Droits : CC-BY-NC-ND - Attribution - Pas d'utilisation commerciale - Pas de modification
Permalien : http://www.manioc.org/fichiers/V15110
V15110
V15110

Éditeur(s) : HAL CCSD American Society for Microbiology
Résumé : International audience
UNLABELLED: During visceral leishmaniasis, the control of hepatic parasite burden is mainly due to granuloma assembly in a microenvironment consisting of both Th1 and Th2 components. Using enzyme-linked immunosorbent assay (ELISA) dosages, quantitative PCR (qPCR), immunohistochemistry, and flow cytometry, we studied the role of interleukin-33 (IL-33), a recently described cytokine signaling through the ST2 receptor, during visceral leishmaniasis. We showed that a higher level of IL-33 was detected in the serum of patients with visceral leishmaniasis than in that from healthy donors and demonstrated the presence of IL-33(+) cells in a liver biopsy specimen from a patient. Similarly, in BALB/c mice experimentally infected with L. donovani, a higher level of IL-33 was detected in the serum, as well as the presence of IL-33(+) cells and ST2(+) cells in the mouse liver. In ST2(-/-) BALB/c mice, better control of the hepatic parasite burden and reduced hepatomegaly were observed. This was associated with strong induction of Th1 cytokines (gamma interferon [IFN-γ] and IL-12) compared to the level in wild-type (WT) mice and better recruitment of myeloid cells associated with strongly induced chemokines (CCL2 and CXCL2) and receptors (CCR2 and CXCR2). Conversely, BALB/c mice treated twice weekly with recombinant IL-33 showed a dramatically reduced induction of Th1 cytokines and delayed inhibition of monocyte and neutrophil recruitment in the liver, which was associated with reduced KC/CXCL1 and CXCR2 expression. Taken together, our results suggest that IL-33 could be a new deleterious regulator of the hepatic immune response against Leishmania donovani, via the repression of the Th1 response and myeloid cell recruitment. IMPORTANCE: Visceral leishmaniasis is a life-threatening systemic disease due to the Leishmania protozoa L. infantum and L. donovani and is ranked by the World Health Organization as the second most important protozoan parasitic disease after malaria for its grave morbidity, high mortality, and global distribution. Leishmania parasites subvert the host's immune response to propagate to target organs, including the spleen, the bone marrow, and the liver. Control of hepatic parasite burdens depends on a delicate and poorly understood Th1/Th2 immune balance. To better understand this complex immune response, new cytokines are interesting targets for research studies. IL-33 is a newly described cytokine usually associated with Th2 response and involved in different diseases, including infectious diseases and hepatitis. Our results suggest that IL-33 could be a new factor of susceptibility and a potential prognostic marker during visceral leishmaniasis.
ISSN: 2150-7511

inserm-00871493
http://www.hal.inserm.fr/inserm-00871493
http://www.hal.inserm.fr/inserm-00871493/document
http://www.hal.inserm.fr/inserm-00871493/file/mBio.00383-13.pdf
DOI : 10.1128/mBio.00383-13

Éditeur(s) : HAL CCSD
Résumé : International audience
The alarmin IL-33 has been described to be upregulated in human and murine viral hepatitis. However, the role of endogenous IL-33 in viral hepatitis remains obscure. We aimed to decipher its function by infecting IL-33-deficient mice (IL-33 KO) and their wild-type (WT) littermates with pathogenic mouse hepatitis virus (L2-MHV3). The IL-33 KO mice were more sensitive to L2-MHV3 infection exhibiting higher levels of AST/ALT, higher tissue damage, significant weight loss, and earlier death. An increased depletion of B and T lymphocytes, NKT cells, dendritic cells, and macrophages was observed 48 h postinfection (PI) in IL-33 KO mice than that in WT mice. In contrast, a massive influx of neutrophils was observed in IL-33 KO mice at 48 h PI. A transcriptomic study of inflammatory and cell-signaling genes revealed the overexpression of IL-6, TNFα, and several chemokines involved in recruitment/activation of neutrophils (CXCL2, CXCL5, CCL2, and CCL6) at 72 h PI in IL-33 KO mice. However, the IFNγ was strongly induced in WT mice with less profound expression in IL-33 KO mice demonstrating that endogenous IL-33 regulated IFNγ expression during L2-MHV3 hepatitis. In conclusion, we demonstrated that endogenous IL-33 had multifaceted immunoregulatory effect during viral hepatitis via induction of IFNγ, survival effect on immune cells, and infiltration of neutrophils in the liver.
Mediators of Inflammation

hal-01544268
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01544268
DOI : 10.1155/2017/1359064
PUBMED : 28607531
PUBMEDCENTRAL : PMC5457781

Éditeur(s) : HAL CCSD
Résumé : International audience
We present a chronological (K-Ar), petrologic and geochemical study (major and trace elements, Sr-Nd isotopes) of Middle Miocene (ca. 16-14 Ma) calc-alkaline rocks (basalts to andesites) extruded in the present-day back-arc region of Central Patagonia (Zeballos Volcanic Sequence (ZVS), 47 S). This magmatism started shortly after mafic plutonism ceased in the arc region (ca. 16 Ma, 200 km west), and ended ca. 2 My before the onset of voluminous slab tear-related back-arc alkaline basaltic magmatism (ca. 12 to Pliocene). The studied calc-alkaline rocks have a typical subduction-related signature (high LILE/HFSE ratios, depletion in Nb, Ta and Ti; Ba/La >20; Ta/Hf <1.5; (Sr-87/Sr-86)(o)=0.70366-0.70402, epsilon(Nd)=+0.1-+3.8). Major and trace elements contents are consistent with their evolution by closed system fractional crystallization of a presumed parental liquid similar in composition to the most basic rock of the suite. Moreover, a strong subducted sediment imprint is recognized (increasing Th/HFSE and decreasing Ce/Pb during differentiation). However, these rocks show striking similarities with volcanic complexes emplaced above areas where a gently dipping slab occurs (high K contents; similar LREE/HREE, Nb/Zr, Ba/Nb; Th/Hf; Th/Ta, Ta/Hf<0.3), particularly the present-day Andean flat-slab region and the Neuquen Basin during the Late Miocene. A comprehensive tectono-magmatic model is here presented to explain the generation and extrusion of these calc-alkaline magmas during the Middle Miocene. The development of a transient low-angle subduction and the resulting eastward migration of the volcanic front are then proposed. Mixing between stored remnants of calc-alkaline magmatism and later primitive alkaline melts is envisioned as the most likely process accounting for the transitional signature (i.e., intermediate between calc-alkaline and alkaline, La/Nb >I; TiO2 <2 wt%) of some basalts extruded synchronously with genuine alkaline lavas in the Neogene Patagonian Plateau Lavas province.
Volcanismo calcoalcalino durante el Mioceno Medio en Patogonia Central (47°S): petrogenesis e implicaciones en la dinamica de placas. En este trabajo se presenta un estudio cronologico (K-Ar), petrologico y geoquimico (elementos mayores y trazas, isotopos de Sr-Nd) de rocas calcoalcalinas (basaltos a andesitas) del Mioceno Medio (16-14 Ma) que ocurren en una posicion de trasarco en Patagonia Central (47°S; Secuencia Volcanica Zeballos, SVZ). Este magmatismo comenzo poco despees de que el plutonismo mafico cesara en la region bajo el arco (ca. 16 Ma, 200 km al oeste), y termino ca. 2 My antes del comienzo de un voluminoso evento magmatico basaltico alcalino desarrollado en el trasarco, relacionado con ventanas astenosfericas, que continuo hasta el Pleistoceno. Las rocas calcoalcalinas estudiadas tienen una quimica tipicamente relacionada con procesos de subduccion (altas razones LILE/HFSE, empobrecimientos en Nb, Ta y Ti; Ba/La>20; Ta/Hf l,5; (87Sr/86Sr)0=0,70366-0,70402, End~O,1±3,8); y sus contenidos de elementos mayores y trazas son consistentes con una evolucion por cristalizacion fraccionada, en un sistema cerrado, desde un liquido parental de composicion similar a la de la roca mas basica del conjunto analizado. Se reconoce, ademas, la influencia de sedimentos subductados en la composicion de estos magmas (aumento de la razon Tb/HFSE y disminucion de la razon Ce/Pb durante la diferenciacion). Sin embargo, estas rocas muestran importantes similitudes composicionales con las de complejos volcanicos emplazados sobre areas donde ocurre subduccion de bajo angulo (altos contenidos de K, similares razones LREF/HREE, Nb/Zr, Ba/Nb; TblHf; TblTa, Ta/Hf O,3), particularmente rocas de la actual region de subduccion plana en Chile central-Argentina y de la cuenca de Neuquen durante el Mioceno Superior. Se propone aqui un modelo tectono-magmatico que explica la generacion y extension de magmas calcoalcalinos en una posicion de trasarco durante el Mioceno Medio en Patagonia mediante el desarrollo de un fenomeno transiente de subduccion de bajo angulo y el consiguiente desplazamiento hacia el este del frente volcanico. Ademas, la mezcla entre remanentes de este magmatismo calcoalcalino almacenados en la corteza y fundidos alcalinos posteriores, provenientes de la astenosfera, se presenta como el proceso responsable de la generacion de la signatura transicional (i.e., intermedia entre calcoalcalina y alcalina, La/Nb>1; TiO2 2 wt%) reconocida en algunos basaltos que ocurren contemporaneamente con otros puramente alcalinos en la provincia de las Lavas Neogenas de Plateau de la Patagonia.
ANDEAN GEOLOGY

insu-00561888
https://hal-insu.archives-ouvertes.fr/insu-00561888
DOI : 10.5027/andgeo2010-678

Éditeur(s) : HAL CCSD American Society for Microbiology
Résumé : International audience
: We investigated the in vitro effects of four alkyl-galactofuranoside derivatives, i.e. octyl-β-D-galactofuranoside (1), 6-amino-β-D-galactofuranoside (2), 6-N-acetamido-β-D-galactofuranoside (3), and 6-azido-β-D-galactofuranoside (4) on Leishmania donovani. Their mechanism of action was explored using electron paramagnetic resonance spectroscopy (EPR) and nuclear magnetic resonance (NMR), and ultrastructural alterations by transmission electron microscopy (TEM). Compound 1 showed the most promising effects by inhibiting promastigote growth at IC50 8.96 ± 2.5 μM. All compounds exhibit low toxicity towards human macrophages. Compound 1 had a higher selectivity index than the comparative molecule used, i.e. miltefosine (159.7 versus 37.9, respectively). EPR showed that compound 1 significantly reduced membrane fluidity, compared to control promastigotes and to compound 3. The furanose ring was shown to support this effect since the isomer galactopyranose had no effect on parasite membrane fluidity or growth. NMR showed a direct interaction of all compounds (1>2>3>4) with the promastigote membrane, as well as with octyl-galactopyranose and octanol, providing evidence that the n-octyl chain was primarily involved in the anchoring with the parasite membrane, followed by the putative crucial role of furanose ring in the anti-leishmanial activity. A morphologic analysis of compound 1-treated promastigotes by TEM revealed profound alterations on parasite membrane and organelles, but not with compound 3. Quantification of annexinV binding by flow cytometry confirmed that compound 1 induced apoptosis in >90% promastigotes. The effect of compound 1 was also assessed on intra-macrophagic amastigotes, and showed a reduction in amastigote growth associated with an increase of ROS production, thus validating its promising effect.
ISSN: 0066-4804

hal-00941772
https://hal.archives-ouvertes.fr/hal-00941772
PUBMED : 24468785
DOI : 10.1128/AAC.01350-13