Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : Elsevier
Résumé : To better understand the recent motion of the Pacific plate relative to the Rivera plate and to better define the limitations of the existing Rivera-Pacific plate motion models for accurately predicting this motion, total-field magnetic data, multibeam bathymetric data and sidescan sonar images were collected during the BART and FAMEX campaigns of the N/O L'Atalante conducted in April and May 2002 in the area surrounding the Moctezuma Spreading Segment of the East Pacific Rise, located offshore of Manzanillo, Mexico, at 106 degrees 16'W, between 17.8 degrees N and 18.5 degrees N. Among the main results are: (1) the principle transform displacement zone of the Rivera Transform is narrow and well defined east of 107 degrees 15'W and these azimuths should be used preferentially when deriving new plate motion models, and (2) spreading rates along the Moctezuma Spreading Segment should not be used in plate motion studies as either seafloor spreading has been accommodated at more than one location since the initiation of seafloor spreading in the area of the Moctezuma Spreading Segment, or this spreading center is not a Rivera-Pacific plate boundary as has been previously assumed. Comparison of observed transform azimuths with those predicted by the best-fit poles of six previous models of Rivera-Pacific relative motion indicate that, in the study area, a significant systematic bias is present in the predictions of Rivera-Pacific motion. Although the exact source of this bias remains unclear, this bias indicates the need to derive a new Rivera-Pacific relative plate motion model.
Tectonophysics (0040-1951) (Elsevier), 2008-06 , Vol. 454 , N. 1-4 , P. 70-85

Droits : 2008 Elsevier B.V. All rights reserved.
http://archimer.ifremer.fr/doc/2008/publication-4486.pdf
DOI:10.1016/j.tecto.2008.04.013
http://archimer.ifremer.fr/doc/00000/4486/

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
Selected high-resolution seismic-reflection profiles and multibeam bathymetry acquired along the convergent Ecuador margin during the ATACAMES cruise on onboard the R/V L’Atalante (Jan.15–Feb.18, 2012) allow a preliminary evaluation of the neotectonic development and stratigraphic evolution of the margin based on the sismo-stratigraphic analysis of Quaternary sediment preserved on the margin shelf and upper slope. We present three major preliminary results. (1) The evolution of the Esmeraldas, Guayaquil and Santa Elena canyons. The head of the Esmeraldas canyon is the location of a continuous significant sediment transport. The Guayaquil canyon shows several episodes of deposition and incision. Aggrading sedimentation pattern in the canyon records several changes in relative sea-level. The subsidence of the Gulf of Guayaquil probably contributes to the good preservation of the canyon filling stages. The Santa Elena canyon is controlled by a SW–NE trending normal fault. (2) Variations of sediment accumulation and relative vertical motions are shown along-strike the shelf edge. Offshore the uplifted Manta peninsula, a pronounced subsidence of the shelf edge is documented by sedimentary clinoforms that have deposited in a morphological reentrant, and have migrated upslope testifying of a local subsidence meanwhile the adjacent La Plata Island area underwent uplift. In the Esmeraldas canyon area, a local uplift of the shelf is documented. (3) Two neotectonic fault systems with a possible transcurrent component are imaged across the shelf edge and upper margin slope offshore Jama, and Cape Galera. This possible transcurrent motion could be related to the reactivation of ancient faults of the upper plate by the subduction. These preliminary results indicate that the ATACAMES data set has a strong potential to evaluate the spatial and temporal contribution of tectonic and climate changes on the structural development and stratigraphic evolution of the Ecuador continental margin.
ISSN: 0025-3235

insu-01131151
https://hal-insu.archives-ouvertes.fr/insu-01131151
DOI : 10.1007/s11001-014-9231-y