Éditeur(s) :
HAL CCSD Biophysical Society Résumé : International audience
SHG angular intensity pattern (SHG-AIP) of healthy and proteolysed muscle tissues are simulated and imaged here for the first time to our knowledge. The role of the spatial distribution of second-order nonlinear emitters on SHG-AIP is highlighted. SHG-AIP with two symmetrical spots is found to be a signature of healthy muscle whereas SHG-AIP with one centered spot in pathological mdx muscle is found to be a signature of myofibrillar disorder. We also show that SHG-AIP provides information on the three-dimensional structural organization of myofibrils in physiological and proteolysed muscle. Our results open an avenue for future studies aimed at unraveling more complex physiological and pathological fibrillar tissues organization.
ISSN: 0006-3495
hal-00825645
https://hal.archives-ouvertes.fr/hal-00825645 https://hal.archives-ouvertes.fr/hal-00825645/document https://hal.archives-ouvertes.fr/hal-00825645/file/Theoretical-SHG-accepted.pdf DOI : 10.1016/j.bpj.2013.02.047