Etudes sur les mortalitees larvaires de la coquille st Jacques Pecten maximus en ecloserie Auteur(s) : Robert, Rene Miner, Philippe Nicolas, Jean-louis Mazuret, Michel Connan, Jean-paul Résumé : Larval mortalities occurring in molluscan hatcheries have often been associated with bacterial contamination. Although batches of oyster and clam larvae have been routinely reared in the hatchery of Argenton without antibiotics, high larval mortalities have been recorded with Pecten maxirnus under similar conditions. For this species, an addition of chloramphenicol was found necessary. However, this chemical has now been banned in Europe. Thus either substitition products or an improvement in the rearing procedures is essential. Studies carried out have shown that neither a decrease in larval density (1 larvae. ml"l) nor sea water change frequency (1 per day) had any positive effects. Furthermore, elective substances such as sugars were not suitable and the use of another antibiotic, erytromvcine. led to inconsistent results- En ecloserie de mollusques, les mortalités larvaires sont souvent associées à des proliférations bactériennes. Pour les limiter un traitement de l'eau est incontournable et chez certaines espèces, dont la coquille St Jacques Pecten maximus, l'utilisation de chloramphenicol s'avère nécessaire. Or son usage dans les productions animales a été récemment prohibé par la communauté européenne. Afin de pallier à cette interdiction, l'amélioration des procédures zootechniques ainsi que l'utilisation de produits de substitution ont été effectuées. Quel que soit le procédé envisagé, abaissement de la densité en élevage, augmentation de la fréquence du renouvellement de l'eau d'élevage, mise au point de filtres biologiques, utilisation de substances électives, les élevages larvaires n'ont pu être menés à terme. A l'inverse, de bons développement larvaires ont été obtenus avec adjonction d'érythromycine mais ces résultats ne sont pas suffisamment reproductibles pour que cet antibiotique constitue un produit de substitution efficace. Droits : info:eu-repo/semantics/openAccess http://archimer.ifremer.fr/doc/1995/rapport-1568.pdf http://archimer.ifremer.fr/doc/00000/1568/ | Partager Voir aussi Mortality Pecten maximus Hatchery Larvae Mollusc Mortalité Pecten maximus Ecloserie Larve Mollusque Télécharger |
Interspecific comparison of Cd bloaccumulation in European Pectinidae (Chlamys varia and Pecten maximus) Auteur(s) : Metian, M Warnau, M Oberhansli, F Teyssie, J Bustamante, Paco Éditeur(s) : Elsevier Résumé : The uptake and loss kinetics of Cd were determined in two species of scallops from the European coasts, the variegated scallop Chlamys varia and the king scallop Pecten maximus, following exposures via seawater, phytoplankton and sediment using highly sensitive radiotracer techniques (Cd-109). Results indicate that, for seawater and dietary pathways, C. varia displays higher bioaccumulation capacities in terms of uptake rate from water and fraction absorbed from ingested food (assimilation efficiency) than Pecten maximus. Regarding sediment exposure, P. maximus displayed low steady-state Cd transfer factor (TFSS < 1); however, once incorporated, a very large part of Cd transferred from sediment (92%) was strongly retained within R maximus tissues. Both species showed a high retention capacity for Cd (biological half-life, T-b1/2 > 4 months), suggesting efficient mechanisms of detoxification and storage in both species. The digestive gland was found to be the main storage organ of Cd in the two scallops regardless of the exposure pathway. However, Cd was stored differently within this organ according to the species considered: 40% of the total Cd was found in the soluble cellular fraction in C. varia whereas this soluble fraction reached 80% for P. maximus. This suggests that the two species displayed different Cd detoxification/storage mechanisms. Finally, the present study has determined the relative contribution of the different exposure pathways to global Cd bioaccumulation for the two scallop species. Results clearly show that for both species, food constitutes the major accumulation pathway, contributing for >99% and 84% of the global Cd bioaccumulation in C. varia and R maximus, respectively. This work confirms the previous assumption, derived from a bibliographic overview, that dietary pathway plays a prevalent role in metal bioaccumulation in Pectinidae. (C) 2007 Elsevier B.V. All rights reserved. Journal of Experimental Marine Biology and Ecology (0022-0981) (Elsevier), 2007-12 , Vol. 353 , N. 1 , P. 58-67 Droits : 2007 Elsevier B.V. All rights reserved. http://archimer.ifremer.fr/doc/2007/publication-3581.pdf DOI:10.1016/j.jembe.2007.09.001 http://archimer.ifremer.fr/doc/00000/3581/ | Partager |
Effect of sampling location, release technique and time after activation on the movement characteristics of scallop (Pecten maximus) sperm Auteur(s) : Suquet, Marc Quere, Claudie Mingant, Christian Lebrun, Luc Ratiskol, Dominique Miner, Philippe Cosson, Jacky Éditeur(s) : Edp Sciences S A Résumé : Sperm characteristics of scallops have not been well described in the scientific literature. The effects of sperm release technique (thermal shock versus serotonin injection), of sperm collection technique (testis sampling versus serotonin injection), of sperm sampling location along the genital tract, of in vitro sperm maturation, and of time post activation on scallop sperm characteristics were assessed in the present work. Whatever sperm release technique used, no significant differences were observed regarding the percentage of motile spermatozoa and the velocity of the average path (YAP). Compared to testicular sperm, a higher percentage of motile spermatozoa, YAP and intracellular adenosine triphosphate (ATP) content were observed for sperm shed after serotonin injection. From the distal part of testes up to the gonopore, an increase of the percentage of motile spermatozoa and YAP was assessed, suggesting a sperm 'maturation process' along the genital ducts. A higher increase in the percentage of motile sperm was recorded during a 5 mm incubation of testicular sperm in seawater containing 2 mM serotonin and seawater containing 10 mM caffein compared to seawater (control). In addition, a higher YAP was assessed, incubating testicular sperm in caffein, compared to control or serotonin. Then, the percentage of motile spermatozoa, YAP and intracellular ATP content exhibited a progressive reduction during the 10 h swimming period. Mean values of the percentage of motile spermatozoa, YAP, sperm track linearity (LIN) and intracellular ATP content recorded at the beginning of the movement period for sperm samples collected after intragonadal serotonin injection, were 82 +/- 7%, 162 +/- 15 mu m s(-1), 0.33 +/- 0.12 and 212 +/- 133 nmol x 10(-9) spermatozoa (n = 9 males), respectively. The present study confirms the existence of a sperm "maturation process" along scallop genital ducts. In addition, the cessation of scallop sperm movement can be explained by the exhaustion of ATP content at the end of the movement phase. Aquatic Living Resources (0990-7440) (Edp Sciences S A), 2013-07 , Vol. 26 , N. 3 , P. 215-220 Droits : EDP Sciences, IFREMER, IRD 2013 http://archimer.ifremer.fr/doc/00157/26852/24983.pdf DOI:10.1051/alr/2013048 http://archimer.ifremer.fr/doc/00157/26852/ | Partager |
Observations on larval development and settlement of Patinofecten yessoensis in hatcheries Auteur(s) : Robert, Rene Miner, Philippe Mazuret, Michel Connan, Jean-paul Éditeur(s) : Proceedings of the 9th International Pectinid Workshop, Canada Résumé : The influence of density on Patinopecten yessoensis larval development was studied during production of an F2 generation. Setting behaviour of P. yessoensis on PVC removable spat collectors, previously tested with Pecten maximus, was determined. Poor larval growth of P. yessoensis was observed at a larval density of 7 ·ml·1 and post larval settlement occurred mainly on the bottom of the baskets, as has been observed with the king scallop, P. maximus. The PVC collector is not suitable for either species of scallop. Droits : 1994 Ifremer http://archimer.ifremer.fr/doc/00090/20163/17816.pdf http://archimer.ifremer.fr/doc/00090/20163/ | Partager |