Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD American Geophysical Union (AGU)
Résumé : International audience
We use digital elevation models and satellite imagery data completed with field observations to develop an integrated morphotectonic analysis of the anomalous topography of the Central Cordillera of Patagonia between 46 degrees S and 48 degrees S, in a region overlying subducted segments of the Chile Spreading Ridge. This region shows a number of peculiar features, not expected in a normal subduction-related belt located away from a buried spreading center. First, it is characterized by overall short-wavelength topography and highly contrasted reliefs, typical of deeply incised young mountain belts, but surprisingly, also by an average very low elevation of similar to 900 m. Second, the Cordillera lacks central high reliefs; instead, there is a central, depressed domain with average low slope gradients including the deepest point of the study area (300 m below sea level in the bottom of Lake General Carrera/Buenos Aires). This depressed area is flanked by western and eastern regions of higher elevation having opposite general slope dips. To the east, the central Patagonian Cordillera is now separated from the flat plains of the Pampean domain (representing its former piedmont) and several elevated basaltic plateaus (mesetas) by 160 degrees N-180 degrees N trending morphotectonic corridors and the western border scarps of the mesetas. These scarps correspond to neotectonic features controlled by recent vertical faults, mostly active after 3 Ma. Remnants of a former peneplain, the Avellanos surface, are widespread within the entire Cordilleran region. From geological evidence, this peneplain formed between 15 and 4 Ma and was connected originally with the mesetas domain and now is dissected by these corridors. It is shown that a negative inversion related to extensional and strike-slip faulting occurred in the frontal region of the Cordillera at 3 Ma synchronously with the emplacement of magmatic products. Timing of the extensional deformation in central Patagonia is concomitant with the migration at depth of different segments of the South Chile Ridge, which entered the trench at 6 and 3 Ma, respectively, and the consequent opening of a slab window allows asthenospheric hot mantle to reach lithospheric regions. Finally, migration of segments of the Chile Spreading Ridge since 6 Ma triggered regional uplift of the Cordillera followed by limited extension in relation with increased thermal weakening of the crust.
ISSN: 0278-7407

hal-00486334
https://hal.archives-ouvertes.fr/hal-00486334
DOI : 10.1029/2009TC002453