Éditeur(s) : University of Florida University of Florida ( Gainesville, Fla. )
Résumé : (Thesis) Thesis (Ph. D.)--University of Florida, 1965.
(Bibliography) Includes bibliographical references (leaves 93-96).
Typescript.
Vita.
(Statement of Responsibility) by Richard Terrance Arbogast.
UF

Droits : Copyright [name of dissertation author]. Permission granted to the University of Florida to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
000432036
37517404
ACJ1540

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD American Geophysical Union
Résumé : International audience
The 856 A.D. Qumis earthquake (M7.9) is the most destructive earthquake to have occurred in Iran, killing more than 200,000 people and destroying the cities of Damghan and the old Parthian capital of Shahr-i Qumis (Hecatompylos). This study combines evidence of historical seismicity with observations of the geomorphology and paleoseismology to provide the first detailed description of active faulting in the Damghan region of the east Alborz mountains, Iran. Regional left-lateral shear is accommodated on the Astaneh, Damghan, and North Damghan faults. Quaternary alluvial fans have been displaced along the Astaneh fault, with 15–20 m stream offsets recording the cumulative displacement over the last two to five earthquakes. A paleoseismology study from a single trench along a 5–10 km segment of the Astaneh fault reveals a rupture prior to 1300 A.D. and significantly later than 600 B.C. Despite the limitations of a single trench in documenting the spatial and temporal evolution of the fault over the late Quaternary, we are nevertheless able to bracket the last event to a time period consistent with the 856 A.D. earthquake. Two older earthquakes were also identified during the Holocene occurring between 600 B.C. and 4600 B.C. and between 4600 B.C. and 9600 B.C. The location of our trench within a bend on the Astaneh fault, which could act as a barrier to rupture propagation, means the three earthquakes recovered from our trench over the Holocene may represent a minimum. Further trenching will reveal how the Astaneh fault ruptures over repeated earthquakes and, consequently, the magnitude and extent of slip during the 856 A.D. earthquake.
ISSN: 0148-0227

hal-00551330
https://hal.archives-ouvertes.fr/hal-00551330
https://hal.archives-ouvertes.fr/hal-00551330v2/document
https://hal.archives-ouvertes.fr/hal-00551330/file/Hollingsworth2010.pdf
DOI : 10.1029/2009JB007185