Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
A data stream is a potentially uninterrupted flow of data. Mining this flow makes it necessary to cope with uncertainty, as only a part of the stream can be stored. In this paper, we evaluate a statistical technique which biases the estimation of the support of patterns, so as to maximize either the precision or the recall, as chosen by the user, and limit the degradation of the other criterion. Theoretical results show that the technique is not far from the optimum, from the statistical standpoint. Experiments performed tend to demonstrate its potential, as it remains robust even under significant distribution drifts.
ISSN: 0031-3203

lirmm-00365474
https://hal-lirmm.ccsd.cnrs.fr/lirmm-00365474

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
BACKGROUND: Surveillance of HIV-1 drug resistance in treated patients with plasma viral load (VL) >50 copies/mL. METHODS: The protease and reverse transcriptase (RT) genes were systematically sequenced in samples from 756 patients with VL >50 copies/mL in 2009. The genotyping results were interpreted for each antiretroviral drug (ARV) by using the ANRS algorithm v21. Weighted analyses were used to derive representative estimates of percentages of patients. Prevalence rates were compared with those obtained in 2004 among patients with VL >1000 copies/mL. RESULTS: Sequences were obtained for 506 patients. Sequencing was successful in 45%, 80% and 96% of samples with VL of 51-500, 501-1000 and >1000 copies/mL, respectively. Resistance or possible resistance to at least one ARV was observed in 59% of samples. Overall, 0.9% of samples contained viruses resistant to all drugs belonging to at least three drug classes. All resistance prevalence rates were significantly lower in 2009 than in 2004. CONCLUSION: In France, where 86% of patients were receiving combination antiretroviral therapy in 2009, only 15.0% of patients had a VL >50 copies/mL, suggesting that only 8.9% of treated patients could potentially transmit resistant viruses. Only 0.08% of patients harboured viruses fully resistant to at least three antiretroviral drug classes. Further studies are needed to determine whether resistance continues to decline over time.
ISSN: 0305-7453

hal-00875092
https://hal.archives-ouvertes.fr/hal-00875092
DOI : 10.1093/jac/dkt033
PUBMED : 23404192

Éditeur(s) : HAL CCSD Oxford University Press (OUP)
Résumé : International audience
We investigate the deformation induced by water pressure variations in hydrologically active natural fractures, and recorded by tiltmeters and strainmeters. The deformation associated with a single fracture is derived using finite-element modelling (FEM). A range in fracture geometries is explored, first to highlight the sensitivity of each geometrical parameter to the deformation, and secondly to allow transfer to observation sites. Water level variations in the fracture are then derived from a hydrological model, driven by observed rainfall, and calibrated on fracture water flow measurements. The modelling results are explicitly applied to constrain the local hydrological contribution to observations with the 100-m-long hydrostatic tiltmeter installed at Sainte-Croix-aux-Mines (France). Our study shows that well-founded physical modelling of local hydrological effect allows a substantial correction of records in observatories.
ISSN: 0956-540X

hal-00708093
https://hal.archives-ouvertes.fr/hal-00708093
https://hal.archives-ouvertes.fr/hal-00708093/document
https://hal.archives-ouvertes.fr/hal-00708093/file/fracture_v72.pdf
BIBCODE : 2009GeoJI.178..667L

Éditeur(s) : HAL CCSD
Résumé : International audience
Mass balances are often used to calculate sediment fluxes in sedimentary basins and denudationrates in adjacent topographies on intermediate to long timescales (from a few tens ofthousand to a million years). In this study, we focus on simple Quaternary catchment–alluvialfan systems in the northern Tian Shan Range and its foreland basin to discuss some ideasabout sediment production, storage, release, and bypass in relatively short (100 km long) sedimentrouting systems. Based on a geometrical reconstruction of the fans, we estimated thevolumes of sediments exported out of the range and deposited in its alluvial piedmont duringthe Middle-Late Pleistocene and the Holocene. This work shows that the two areas clearlypresent evidence of a temporary but significant storage of sediments during the Pleistocene.These sediments were then excavated and delivered farther into the foreland basin during theHolocene. The difference between the volumes of materials released from the range and piedmontareas and the volume stored in the contemporaneous fans downstream indicates thatthe latter did not trap the whole sediment load transported by the rivers. The alluvial fans werebypassed by 27 to 78% of this load toward the downstream alluvial plain. It implies a majorvolumetric partitioning of the deposits between the fans and the alluvial plain, with a sedimentationrate about twenty times higher in the fans than in the plain. However, this volumetricpartitioning might only occur during periods with a very specific hydrological regime suchas the Holocene deglaciation. Eventually, the peculiar sediment storage and release patternwithin the range and piedmont areas during the Pleistocene and Holocene complicates thecalculation of mean paleodenudation rates using either sediment budgets or in situ producedcosmogenic nuclides.
Source to Sink: a long term perspective of sediment budgets and sources characterization, Abstracts
Rennes, France

insu-01406440
https://hal-insu.archives-ouvertes.fr/insu-01406440

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The paleostorm history of coastal lagoon environments (Pierre Blanche Lagoon, central part of the Gulf of Lions) has been established by integrating clay mineralogy and geochemical analysis. Clay mineralogy combined with bulk major and trace elements concentrations allow for the definition of four different end members: the Mosson drainage basin, the sandy barrier, the biogenic and the anthropic components. The two main sedimentary sources of the lagoonal system are the Mosson drainage basin which has a high concentration of smectite and Al2O3, and the sandy barrier characterized by high contents of illite, chlorite, SiO2 and Zr. Smectite/(illite + chlorite), SiO2/Al2O3 and Zr/Al2O3 ratios can be used to reconstruct the past storm history of the Pierre Blanche Lagoon. Our results indicate that the sensitivities of the clay mineralogy and geochemistry proxies are different. These sensitivities could be related: 1) first, to the comparison between different grain size for geochemistry and for clay mineralogy: 2) second, to the complexity of the mix (four end-members for the geochemistry and two end-members for the clay minerals): and 3) third, by the storm's ability to transport sediment of different sizes. (C) 2010 Elsevier B.V. All rights reserved.
ISSN: 0037-0738

hal-00512614
https://hal.archives-ouvertes.fr/hal-00512614
DOI : 10.1016/j.sedgeo.2010.04.012

Éditeur(s) : HAL CCSD Elsevier Masson
Résumé : International audience
The only symbiotic Mediterranean gorgonian, Eunicella singularis, has faced several mortality events connected to abnormal high temperatures. Since thermotolerance data remain scarce, heat-induced necrosis was monitored in aquarium by morphometric analysis. Gorgonian tips were sampled at two sites: Medes (Spain) and Riou (France) Islands, and at two depths: 15 m and-35 m. Although coming from contrasting thermal regimes, seawater above 28 degrees C led to rapid and complete tissue necrosis for all four populations. However, at 27 degrees C, the time length leading to 50% tissue necrosis allowed us to classify samples within three classes of thermal sensitivity. Irrespectively of the depth, Medes specimens were either very sensitive or resistant, while Riou fragments presented a medium sensitivity. Microsatellite analysis revealed that host and symbiont were genetically differentiated between sites, but not between depths. Finally, these genetic differentiations were not directly correlated to a specific thermal sensitivity whose molecular bases remain to be discovered.
ISSN: 1631-0691

hal-01546168
https://hal.archives-ouvertes.fr/hal-01546168
DOI : 10.1016/j.crvi.2013.06.008

Éditeur(s) : HAL CCSD
Résumé : International audience
New inclinometers devoted to hydrological studies were set up in the Vosges Mountains (France). Two orthogonal 100-meter base hydrostatic inclinometers were installed in December 2004 as well as a hydrometeorological monitoring system for the 100-km² hydrological unit around the inclinometer. As inclinometers are very sensitive to environmental influences, this observatory is a test site to confront hydrological modelling and geodetic observations. Physical modelling to remove hydrological effects without calibrating on geodetic data is tested on these instruments. Specifically, two deformation processes are most important: fluid pressure variations in nearby hydraulically active fractures and surface loading at regional scale.
International Association of Geodesy Symposia
Perugia, Italy

hal-00710442
https://hal.archives-ouvertes.fr/hal-00710442
https://hal.archives-ouvertes.fr/hal-00710442/document
https://hal.archives-ouvertes.fr/hal-00710442/file/2007IUGG_proceedingsIAG.pdf
DOI : 10.1007/978-3-540-85426-5_63

Éditeur(s) : HAL CCSD
Résumé : For operational forecasts anticipate convective inhibition processes is as important than convective initiation processes. Based on the 15th July 2007 case study this work shows how the morning shallow convection over the Vosges is inhibited while it develops over the Black Forest. This case occurs during IOP 8b of the Convective and Orographically-Induced Precipitation Study (COPS). This international field campaign took place during the summer of 2007 over the Vosges, the Rhine Valley and the Black-Forest. The aim of this intensive observation campaign was to study various phases of convection and convective environment and improve quantitative precipitation forecasts. Thanks to GPS tomography data the study show the differences of low-level water-vapor variability between Vosges and Black-Forest. The analysis of ground based and airborne lidar data suggests the importance of dry air layers advected from the south and the influence of the mountains and diurnal cycle of mountain breeze which interfere strongly with these layers preventing the dry air to reach the eastern side of the mountain and the Black Forest. This dynamics is confirmed by models, wind profiler and radiosounding data. So we show how the inhibition of the convection over the Vosges during this case study can be explained with the water vapor variability due to advection and local boundary layer processes.
31st ICAM (International Conference on Alpine Meteorology)
Aviemore, United Kingdom

hal-00668829
https://hal.archives-ouvertes.fr/hal-00668829

Éditeur(s) : HAL CCSD Société géologique de France
Résumé : International audience
The central part of the Gulf of Lions shoreline is characterized by many coastal wetlands that resulted from the interaction between a process of shoreline regularization by migrations of littoral barriers and a slow filling of the back-barrier areas by the riverine and marine inputs. Analyses of Late-Holocene deposits with a very high-resolution multi-proxy study of two sediment cores, allow us to reconstruct the evolution of this coastal system. Two main Holocene sediment units are identified overlying a Pliocene carbonate continental formation. The lower unit consists of sandy and pebbly marine sediments deposited around 7800 B.P., during the final stand of the last sea level rise. Just above, the upper unit displays lagoonal grey clay silts with shells and some intercalated layers of silty sands related to paleostorm events. The age model was established from radiocarbon dating, for the oldest part of the core. Over the last century, sedimentation rates were calculated using the CFCS Pb-210 model, together with Cs-137 data. Radiocarbon data show an increase in he accumulation rate from the base to the top of cores. Marine sand units related to the last transgressive deposit allow to refine the curve of Holocene post-glacial sea level rise. Sedimentological and faunal analyses associated with chronological data provide a means for reconstructing the Late-Holocene paleoenvironments along this part of the coast and suggest that the final closure of the coastal lagoon by the sandy barrier occurred at around 730 +/- 120 yr cal B.P. The beginning of this closure, together with the progradation of the coastal plain, could be responsible for the decline in economic activity of the Lattara harbour during the Roman period.
La partie centrale du littoral du golfe du Lion est caractérisée par de nombreuses lagunes qui résultent de l'interaction entre un processus de régularisation du trait de côte par migration de barrières littorales et d'un lent remplissage des zones situées en arrière des barrières par des apports continentaux et marins. Les analyses des dépôts tardi-holocènes avec une étude multi-traceurs à très haute résolution de deux carottes sédimentaires nous permettent de reconstruire l'évolution de ce système côtier. Deux unités sédimentaires holocènes sont identifiées recouvrant une formation pliocène carbonatée. L'unité inférieure est constituée de sables et de galets d'origine marine déposés vers 7800 B.P., durant les stades ultimes de la dernière remontée marine. Audessus, l'unité supérieure montre des dépôts d'argile et de silt gris coquilliers d'origine lagunaire, dans lesquels sont intercalés des niveaux de sable silteux révélant la présence d'événements de tempêtes. Le modèle d'âge est établi à partir de dates radiocarbones pour les parties les plus anciennes des archives sédimentaires, tandis que pour le dernier siècle, les taux de sédimentation sont calculés en utilisant le modèle CFCS pour le 210Pb, associé aux données de 137Cs. Les données 14C montrent une augmentation du taux d'accumulation depuis la base jusqu'en surface des carottes. Les unités de sables marins liées aux dépôts de la dernière transgression marine permettent de préciser la courbe post-glaciaire de remontée du niveau marin. Les analyses sédimentologiques et faunistiques associées aux données chronologiques fournissent une méthode permettant de reconstituer les paléoenvironnements littoraux tardi-holocènes dans la zone d'étude et suggèrent que la fermeture finale de ces lagunes par le cordon sableux survient autour de 730 ± 120 ans cal B.P. Le début de cette fermeture, associée à la une progradation deltaïque, pourrait être responsable du déclin des activités économiques du port de Lattara durant la période Romaine.
EISSN: 1777-5817

halshs-00740923
https://halshs.archives-ouvertes.fr/halshs-00740923
DOI : 10.2113/gssgfbull.181.1.27

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Syn-orogenic detachments in accretionary wedges make the exhumation of high-pressure and low-temperature metamorphic rocks possible with little erosion. The velocity of exhumation within the subduction channel or the accretionary complex, and thus the shape of P-T paths, depend upon the kinematic boundary conditions. A component of slab retreat tends to open the channel and facilitates the exhumation. We document the effect of slab retreat on the shape of P-T paths using the example of the Phyllite-Quartzite Nappe that has been exhumed below the Cretan syn-orogenic detachment during the Miocene in Crete and the Peloponnese. Data show a clear tendency toward colder conditions at peak pressure and during exhumation where the intensity of slab retreat is larger. This spatial evolution of P-T gradient is accompanied with an evolution from a partly coaxial regime below the Peloponnese section of the detachment toward a clearly non-coaxial regime in Crete.
ISSN: 0040-1951

insu-00411771
https://hal-insu.archives-ouvertes.fr/insu-00411771
https://hal-insu.archives-ouvertes.fr/insu-00411771/document
https://hal-insu.archives-ouvertes.fr/insu-00411771/file/Jolivet-Tectonophysics-2010.pdf
DOI : 10.1016/j.tecto.2009.10.002

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Whereas it is well established that prenatal exposure to polychlorinated biphenyls (PCBs) can disrupt children's behavior, early postnatal exposure has received relatively little attention in environmental epidemiology. To evaluate prenatal and postnatal exposures to PCB-153, a proxy of total PCB exposure, and their relation to inattention and activity in 5-year-old Inuits from the Cord Blood Monitoring Program. Prenatal exposure to PCBs was informed by cord plasma PCB-153 levels. We used a validated pharmacokinetic model to estimate monthly infants' levels across the first year of life. Inattention and activity were assessed by coding of video recordings of children undergoing fine motor testing. We used multivariable linear regression to evaluate the association between prenatal and postnatal PCB-153 levels and inattention (n=97) and activity (n=98) at 5years of age. Cord plasma PCB-153 was not associated with inattention and activity. Each interquartile range (IQR) increase in estimated infant PCB-153 levels at 2months was associated with a 1.02% increase in the duration of inattention (95% CI: 0.04, 2.00). Statistical adjustment for the duration of breastfeeding slightly increased regression coefficients for postnatal level estimates, some of which became statistically significant for inattention (months: 2-4) and activity (months: 2-5). Our study adds to the growing evidence of postnatal windows of development during which children are more susceptible to neurotoxicants like PCBs.
ISSN: 0160-4120

hal-01134338
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134338
DOI : 10.1016/j.envint.2015.02.004
PUBMED : 25796081

Éditeur(s) : HAL CCSD Nature Publishing Group
Résumé : International audience
Cancer cells display alterations in many cellular processes. One core hallmark of cancer is the Warburg effect which is a glycolytic reprogramming that allows cells to survive and proliferate. Although the contributions of environmental contaminants to cancer development are widely accepted, the underlying mechanisms have to be clarified. Benzo[a]pyrene (B[a]P), the prototype of polycyclic aromatic hydrocarbons, exhibits genotoxic and carcinogenic effects, and it is a human carcinogen according to the International Agency for Research on Cancer. In addition to triggering apoptotic signals, B[a]P may induce survival signals, both of which are likely to be involved in cancer promotion. We previously suggested that B[a]P-induced mitochondrial dysfunctions, especially membrane hyperpolarization, might trigger cell survival signaling in rat hepatic epithelial F258 cells. Here, we further characterized these dysfunctions by focusing on energy metabolism. We found that B[a]P promoted a metabolic reprogramming. Cell respiration decreased and lactate production increased. These changes were associated with alterations in the tricarboxylic acid cycle which likely involve a dysfunction of the mitochondrial complex II. The glycolytic shift relied on activation of the Na+/H+ exchanger 1 (NHE1) and appeared to be a key feature in B[a]P-induced cell survival related to changes in cell phenotype (epithelial-to-mesenchymal transition and cell migration).
ISSN: 2045-2322

Droits : http://creativecommons.org/licenses/by/
hal-01359569
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01359569
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01359569/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01359569/file/The%20environmental%20carcinogen-CCBY%20%281%29.pdf
DOI : 10.1038/srep30776

Éditeur(s) : HAL CCSD Lippincott, Williams & Wilkins
Résumé : International audience
BACKGROUND:Fever in infants younger than 3 months is generally a cause for concern because of the risk for a serious bacterial infection. The aim of this study was to describe clinical and biological features of Chikungunya infection in infants < 3 months of age hospitalized in Cayenne Hospital during the 2014-2015 outbreak.METHODS:We performed a preliminary retrospective study followed by a prospective study from March 2014 to February 2015. All infants younger than 3 months presenting with fever and hospitalized in Cayenne Hospital were included. The main diagnostic criteria were fever and positive Chikungunya PCR.RESULTS:One hundred and twenty infants were hospitalized with fever. The mean age was 46 days (SD± 22 days). The mean hospitalization duration was 7.4 days (SD± 6.1 days). Chikungunya infection was diagnosed in 26 children. The most important clinical findings were high (80.8% [77.5-84]) and prolonged fever (76.9%, [73.4-80.4]), irritability (96.2% [94.5-97.7]) and skin rash. (69.2%, [65.4-73]). Half of the infants presented edema of the extremities (hands and feet principally). However, in 15 %, Chikungunya infection was associated with a serious bacterial infection. Infants who presented with irritability, high fever and elevated PCT were at high risk for Chikungunya: OR 39, [9.2-243] (p < 0.001), with a specificity of 96.7 % and a negative predictive value of 89.4%. The area of the ROC curve was 0.96 CONCLUSIONS:: Our results confirm that Chikunguyna infection is a cause of high fever in infants younger than 3 months. Our data should be confirmed by larger studies.
ISSN: 0891-3668

inserm-01476019
http://www.hal.inserm.fr/inserm-01476019
http://www.hal.inserm.fr/inserm-01476019/document
http://www.hal.inserm.fr/inserm-01476019/file/2017%2C%20Elenga%20-%20Chikungunya%20Infection%20in%20hospitalized%20febrile%20infants.pdf
DOI : 10.1097/INF.0000000000001541

Éditeur(s) : HAL CCSD American Society for Microbiology
Résumé : International audience
Toxoplasmosis is a life-threatening infection in immunocompromised patients (ICPs). The definite diagnosis relies on parasite DNA detection, but the incidence and burden of disease are ill-known in HIV-negative patients. A three-year retrospective study was conducted in 15 reference laboratories from the network of the French National Reference Center for Toxoplasmosis, to record the frequency of Toxoplasma DNA detection in ICPs, to review the molecular methods used for diagnosis and the prevention measures implemented in transplant patients. During the study period, out of 31,640 PCR performed in ICPs, 610 were positive (323 patients). Blood (n=337), cerebrospinal fluid (n=101) and aqueous humor (n=100) samples were more frequently positive. Chemoprophylaxis schemes in transplant patients differed between centers. PCR follow-up of allogeneic hematopoietic stem-cell transplant (allo-HSCT) patients was implemented in 8/15 centers. Data from 180 patients (13 centers) were further analyzed regarding clinical setting and outcome. Only 68/180 (38%) patients were HIV+; the remaining 62% consisted in 72 HSCT, 14 solid-organ transplants, and 26 miscellaneous immunodeficiencies. Cerebral toxoplasmosis and disseminated toxoplasmosis were most frequently observed in HIV and transplant patients, respectively. Of 72 allo-HSCT patients with a positive PCR, 23 were asymptomatic; all were diagnosed in centers performing systematic blood PCR follow-up, and received specific treatment. Overall survival of allo-HSCT patients at two months was better in centers with PCR follow-up than in other centers (p<0.01). This study provides updated data on the frequency of toxoplasmosis in HIV-negative ICPs and suggests that regular PCR follow-up of allo-HSCT patients could guide pre-emptive treatment and improve outcome.
ISSN: 0095-1137

hal-01134189
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01134189
DOI : 10.1128/JCM.03282-14
PUBMED : 25762774

Éditeur(s) : HAL CCSD Taylor & Francis
Résumé : International audience
Humans may be exposed via their environment to multiple chemicals as a consequence of human activities and use of synthetic products. Little knowledge is routinely generated on the hazards of these chemical mixtures. The metabolomic approach is widely used to identify metabolic pathways modified by diseases, drugs, or exposures to toxicants. This review, based on the state of the art of the current applications of metabolomics in environmental health, attempts to determine whether metabolomics might constitute an original approach to the study of associations between multiple, low-dose environmental exposures in humans. Studying the biochemical consequences of complex environmental exposures is a challenge demanding the development of careful experimental and epidemiological designs, in order to take into account possible confounders associated with the high level of interindividual variability induced by different lifestyles. The choices of populations studied, sampling and storage procedures, statistical tools used, and system biology need to be considered. Suggestions for improved experimental and epidemiological designs are described. Evidence indicates that metabolomics may be a powerful tool in environmental health in the identification of both complex exposure biomarkers directly in human populations and modified metabolic pathways, in an attempt to improve understanding the underlying environmental causes of diseases. Nevertheless, the validity of biomarkers and relevancy of animal-to-human extrapolation remain key challenges that need to be properly explored.
ISSN: 1093-7404

hal-00976116
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00976116
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00976116/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-00976116/file/Potential_input_from_metabolomics_accepted.pdf
DOI : 10.1080/10937404.2013.860318

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
ISSN: 0257-8972

hal-01679413
https://hal.archives-ouvertes.fr/hal-01679413
DOI : 10.1016/j.surfcoat.2016.03.021

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We review the evolution of concepts on and methods of estimating the state of stress from fault movements. Theories of failure in isotropic materials suggested a simple geometrical construction of optimal principal stress directions from a fault plane and its associated slip. These optimal directions align shear stress and slip directions and maximize the difference between shear stress and frictional resistance on the fault plane. Optimal stress directions for calcite twinning are obtained by a similar construction, with the difference that they maximize shear stress. Force representation of seismic sources independently introduced pressure, P, and tension, T, axes at positions that also maximize shear stress on both nodal planes. Frictional slip theory and the constraint that slip and shear stress directions be parallel allowed to address reactivation of pre-existing faults. This suggested that stress could also be inverted from reactivated fault and slip data or earthquake focal mechanisms. Early methods relied on geometrical constructions as a substitute for calculations, whereas later methods relied on software as these calculations became tractable with the help of computers. Similar methods were developed for the inversion of stress from crystal twin gliding with non-optimal geometry, with a different criterion that relies on a threshold of the component of shear stress along the gliding line. Even though these methods seek a common stress tensor compatible with fault and slip data, their main use is to separate polyphase data into homogeneous subsets and help deciphering complex tectonic histories. Fault and slip data can also be analyzed to constrain the strain rather than the stress tensor. In most cases this involves a summation and yields an average strain for the considered rock volume. Stress inversion thus appears better suited for differentiating heterogeneous data whereas strain analysis appears better suited for homogenizing them.
ISSN: 0040-1951

hal-00759043
https://hal.archives-ouvertes.fr/hal-00759043
DOI : 10.1016/j.tecto.2012.02.009

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Metalloproteases involved in extracellular matrix remodeling play a pivotal role in cell response by regulating the bioavailability of cytokines and growth factors. Recently, the disintegrin and metalloprotease, ADAMTS1 has been demonstrated to be able to activate the transforming growth factor TGF-β, a major factor in fibrosis and cancer. The KTFR sequence from ADAMTS1 is responsible for the interaction with the LSKL peptide from the latent form of TGF-β, leading to its activation. While the atomic details of the interaction site can be the basis of the rational design of efficient inhibitory molecules, the binding mode of interaction is totally unknown. In this study, we show that recombinant fragments of human ADAMTS1 containing KTFR sequence keep the ability to bind the latent form of TGF-β. The recombinant fragment with the best affinity is modeled to investigate the binding mode of LSKL peptide with ADAMTS1 at the atomic level. Using a combined approach with molecular docking and multiple independent molecular dynamics (MD) simulations, we provide the binding mode of LSKL peptide with ADAMTS1. The MD simulations starting with the two lowest energy model predicted by molecular docking shows stable interactions characterized by 3 salt bridges (K3-NH3+ with E626-COO−; L4-COO− with K619-NH3+; L1-NH3+ with E624-COO−) and 2 hydrogen bonds (S2-OH with E623-COO−; L4-NH with E623-COO−). The knowledge of this interaction mechanism paves the way to the design of more potent and more specific inhibitors against the inappropriate activation of TGF-β by ADAMTS1 in liver diseases
ISSN: 1476-9271

hal-01269916
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01269916
DOI : 10.1016/j.compbiolchem.2016.01.012
PUBMED : 26878129

Éditeur(s) : HAL CCSD eLife Sciences Publication
Résumé : International audience
Larval recruitment, the transition of pelagic larvae into reef-associated juveniles, is a critical step for the resilience of marine fish populations but its molecular control is unknown. Here, we investigate whether thyroid-hormones (TH) and their receptors (TR) coordinate the larval recruitment of the coral-reef-fish Acanthurus triostegus. We demonstrate an increase of TH-levels and TR-expressions in pelagic-larvae, followed by a decrease in recruiting juveniles. We generalize these observations in four other coral reef-fish species. Treatments with TH or TR-antagonist, as well as relocation to the open-ocean, disturb A. triostegus larvae transformation and grazing activity. Likewise, chlorpyrifos, a pesticide often encountered in coral-reefs, impairs A. triostegus TH-levels, transformation, and grazing activity, hence diminishing this herbivore’s ability to control the spread of reef-algae. Larval recruitment therefore corresponds to a TH-controlled metamorphosis, sensitive to endocrine disruption. This provides a framework to understand how larval recruitment, critical to reef-ecosystems maintenance, is altered by anthropogenic stressors.
ISSN: 2050-084X

Droits : http://creativecommons.org/licenses/by/
hal-01633712
http://hal.upmc.fr/hal-01633712
http://hal.upmc.fr/hal-01633712/document
http://hal.upmc.fr/hal-01633712/file/elife-27595-v1.pdf
DOI : 10.7554/eLife.27595

Éditeur(s) : HAL CCSD Association Internationale de Climatologie
Résumé : 6 pages
International audience
This work presents an overview of spatio-temporal quality andspatial density of rainfall networks of Cameroon, Gabon, Congo, CAP and DRC, particularly in the recentperiod (1973-2014) through the GSOD international database. Faced with enormous gaps observed, theseresults will help to select metrological and analytical orientations in FORGREENE project (Tropical forestsgreeness and cloudiness in Central Africa: present conditions and future evolution). A comparison with rainfallsatellite estimates (RFE type) also indicates their great potential, provided they are based on in situ validations.
Ce travail fait un état des lieux de la qualité temporelle et de la densité spatiale des sériespluviométriques du Cameroun, Gabon, Congo, RCA et RDC, en particulier sur la période récente (1973-2014)grâce à la base de données internationale GSOD. Face aux énormes lacunes observées, ces résultats vontpermettre d’orienter les choix métrologiques et analytiques in situ du programme FORGREENE (Tropicalforests greeness and cloudiness in Central Africa: present conditions and future evolution). Une comparaisonavec des estimations satellitaires (de type RFE) indique aussi leur potentiel intéressant, à condition qu’elless’appuient sur des validations in situ.
Climat et pollution de l'air
Besançon, France

hal-01360178
https://hal.archives-ouvertes.fr/hal-01360178