Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
: Human exposure to xenobiotics is usually estimated by indirect methods. Biological monitoring has emerged during the last decade to improve assessment of exposure. However, biomonitoring is still an analytical challenge, because the amounts of sample available are often very small yet analysis must be as thorough and sensitive as possible. The purpose of this work was to develop an untargeted "exposomics" approach by using ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS), which was applied to the characterization of pesticide metabolites in urine from pregnant women from a French epidemiological cohort. An upgradable list of pesticides commonly used on different crops, with their metabolites (more than 400 substances) was produced. Raw MS data were then processed to extract signals from these substances. Metabolites were identified by tandem mass spectrometry; putative identifications were validated by comparison with standards and metabolites generated by experiments on animals. Finally, signals of identified compounds were statistically analyzed by use of multivariate methods. This enabled discrimination of exposure groups, defined by indirect methods, on the basis of four metabolites from two fungicides (azoxystrobin, fenpropimorph) used in cereal production. This original approach applied to pesticide exposure can be extended to a variety of contaminant families for upstream evaluation of exposure from food and the environment.
ISSN: 1618-2642

hal-00875889
https://hal.archives-ouvertes.fr/hal-00875889
DOI : 10.1007/s00216-013-7136-2
PUBMED : 23892877

Éditeur(s) : HAL CCSD Elsevier Masson
Résumé : International audience
After noting the forecast of a La Niña episode, associated with heavy rainfall in French Guiana, we monitored the fate of wasp nests before and during the 2006 short rainy season. The population of the most abundant epiponine wasp species, Polybia bistriata, decreased dramatically during the short rainy season (60.6% of the nests disappeared) then remained low for at least 18 months. Colonies that survived moved from the shelter of large, low leaves (a situation well adapted to the previous dry season) of the most frequent substrate tree, Clusia grandiflora (Clusiaceae), to upper leaves, better ventilated and whose orientation provides good protection from the rain. Therefore, the possibility of moving the nest higher during the first rains following the dry season seems very adaptive as colonies that do not do so are eliminated during the La Niña years, whose frequency will increase with global climate change.
ISSN: 1631-0691

hal-00817397
https://hal.archives-ouvertes.fr/hal-00817397
DOI : 10.1016/j.crvi.2009.10.007
PUBMED : 20176334

Éditeur(s) : HAL CCSD
Résumé : International audience
Wound healing is important for sharks from the earliest life stages, for example, as the 'umbilical scar' in viviparous species heals, and throughout adulthood, when sharks can incur a range of external injuries from natural and anthropogenic sources. Despite anecdotal accounts of rapid healing in elasmobranchs, data regarding recovery and survival of individuals from different wound or injury types has not been systematically collected. The present study documented: (i) 'umbilical scar' healing in wild-caught, neonatal blacktip reef sharks while being reared for 30 days in flow-through laboratory aquaria in French Polynesia; (ii) survival and recovery of free-swimming blacktip reef sharks in Australia and French Polynesia following a range of injuries; and (iii) long-term survival following suspected shark-finning activities. Laboratory monitoring, tag-recapture records, telemetry data and photo-identification records suggest that blacktip reef sharks have a high capacity to survive and recover from small or even large and severe wounds. Healing rates, recovery and survival are important factors to consider when assessing impacts of habitat degradation and fishing stress on shark populations. The present study suggests that individual survival may depend more on handling practices and physiological stress rather than the extent of physical injury. These observations also contribute to discussions regarding the ethics of tagging practices used in elasmobranch research and provide baseline healing rates that may increase the accuracy in estimating reproductive timing inferred from mating scars and birth dates for neonatal sharks based on umbilical scar healing status. Cite as: Chin A, Mourier J, Rummer JL (2015) Blacktip reef sharks (Carcharhinus melanopterus) show high capacity for wound healing and recovery following injury. Conserv Physiol 3: doi:10.1093/conphys/cov062.
Conservation physiology

Droits : http://creativecommons.org/licenses/by/
hal-01250745
https://hal-univ-perp.archives-ouvertes.fr/hal-01250745
https://hal-univ-perp.archives-ouvertes.fr/hal-01250745/document
https://hal-univ-perp.archives-ouvertes.fr/hal-01250745/file/Conserv%20Physiol-2015-Chin-.pdf
DOI : 10.1093/conphys/cov062

Éditeur(s) : HAL CCSD Elsevier
Résumé : TAG LITHO
In this study we attempt to understand the water storage variations in a karst aquifer on the Larzac Plateau (South of France) using absolute gravimetry. On this karst system, water input is exclusively rainfall and draining occurs at the Durzon perennial spring in a karstic valley. Our basis assumption is that water storage in the vadose zone (epikarst and infiltration zone) has a significant effect on surface gravity through Newtonian attraction. The karst aquifer is hence being monitored since January 2006 with monthly absolute gravity measurements at three sites. The gravity measurements are corrected for regional scale gravity using water storage models in order to obtain gravity variations related exclusively to the local water storage variations. The gravity variations exhibit an important seasonal component (10-15 mu gal corresponding to a variation of equivalent water stab thickness of similar to 24-36 cm). Water storage variation seen by gravimetry is not spatially uniform on the studied karst system, corroborating current geomorphologic observations and interpretations. Finally, we use a global mass balance consideration linking rainfall, evapotranspiration and spring discharge to provide a conceptual framework for the understanding of observed gravity variations.
ISSN: 0022-1694

hal-00411884
https://hal.archives-ouvertes.fr/hal-00411884
DOI : 10.1016/j.jhydrol.2008.06.020

Éditeur(s) : HAL CCSD
Résumé : Monitoring crustal deformation during the interseismic phase when searching for earth transients requires precise instruments able to operate for very long periods. The ANR-LINES project aimed to develop three new instruments: a single-axis seismometer, a hydrostatic long base tiltmeter and a borehole pendulum tiltmeter. These three instruments benefit of an extrinsic Fabry-Pérot interferometer (EFFPI) with long optic fibers for displacement detections. Their mechanical architectures and their disposal of long fibers help these newly manufactured geophysical instruments complete their goals. The first objective of this study is to propose appropriate methods for estimating the phase of the optical path in the Fabry-Pérot cavities. A modulation of the laser diode current, followed by a homodyne demodulation of the interference signal and a Kalman filter, allow determining the phase in real time. The results are convincingwhile taking short periods measurements but require additional solutions for protection against environmental phenomena variations. The EFFPI sensor integrated in the LINES borehole tiltmeter gives it the opportunity to establish a differential measurement of the bob's oscillation thanks to three Fabry-Perot cavities. The LINES seismometer also uses the EFFPI displacement sensor to measure its coil's displacement. A description of the two instruments' mechanical structures and an analysis of the detected phenomena (slow movements, tides, earthquakes, microseisms . . . ) are part of this thesis.
Le suivi de la déformation de la croûte terrestre durant la phase intersismique pour la recherche des transitoires nécessite des instruments précis capables d'opérer pour de très longues durées. Le projet ANR-LINES a visé le développement de trois nouveaux instruments : un sismomètre mono-axial, un inclinomètre hydrostatique à longue base et un inclinomètre de forage pendulaire. Ces trois instruments profitent d'un capteur interférométrique de déplacement à longues fibres optiques du type Fabry-Pérot Extrinsèque (EFFPI). Leurs architectures mécaniques et l'utilisation de longues fibres permettent à ces instruments géophysiques nouvellement fabriqués d'atteindre les objectifs fixés. Le premier objectif de cette étude est de proposer des méthodes adaptées à l'estimation de la phase du chemin optique dans les cavités Fabry-Pérot. Une modulation du courant de la diode laser, suivie par une démodulation homodyne du signal d'interférence et un filtre de Kalman permettent de déterminer la phase en temps réel. Les résultats sont convaincants pour des mesures de courtes durées mais exigent des solutions complémentaires pour se prémunir des effets de la variation des phénomènes environnementaux. Le capteur EFFPI intégré dans l'inclinomètre de forage LINES lui offre l'opportunité d'établir une mesure différentielle de l'oscillation de la masselotte pendulée grâce à trois cavités Fabry-Pérot. Le sismomètre LINES utilise lui aussi le capteur de déplacement EFFPI pour la mesure du déplacement de sa bobine. Une description de l'architecture mécanique de ces instruments et une analyse des phénomènes détectés (mouvements lents, marées, séismes, microséismes . . . ) font partie de cette thèse.
https://tel.archives-ouvertes.fr/tel-00779335

tel-00779335
https://tel.archives-ouvertes.fr/tel-00779335
https://tel.archives-ouvertes.fr/tel-00779335/document
https://tel.archives-ouvertes.fr/tel-00779335/file/Manuscript_Chawah.pdf

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Almost all Red Sea deeps contain metal-rich sediments covered by brine pools. It is generally agreed that these metal-rich deposits precipitated from overlying metal-rich brines that originated from migrating hydrothermal fluids. No brine pool has ever been reported in Thetis Deep, inciting us to evaluate if such a brine layer ever occurred in the deep during the past. In order to address that questioning, a study combining mineralogical, geochemical (major-, minor-, rare-earth elements) and isotopic (Sr. Nd. Pb) approaches was completed on cored sediments and extracted interstitial waters from inside and outside the deep.;The sediments have an overall hydrothermal origin, as shown by the REE concentrations and patterns, metal contents, and Pb-Nd isotopic data, all pointing to a mantle signature. The intensity of the hydrothermal activity varied with time in the deep; the most intense episode resulting in an almost pure Fe-oxi-hydroxide layer. Varied chemical arguments, especially the Zr and REE data of the sediments, favor the fact that the whole sedimentation in Thetis Deep occurred in the absence of a stable, salt-rich and mineralized brine pool, and that no brine layer ever existed. This conclusion is supported by the constant Sr isotope composition of the sediment and its interstitial waters that are almost identical to that of the Red Sea seawater. The study also suggests that hydrothermal activity monitored fluid supplies that interacted differently with seawater in the different Red Sea deeps, resulting in an overall formation of metal-rich sediments, but along varied local conditions. (C) 2009 Elsevier B.V. All rights reserved.
ISSN: 0375-6742

hal-00475646
https://hal.archives-ouvertes.fr/hal-00475646
DOI : 10.1016/j.gexplo.2009.10.001

Éditeur(s) : HAL CCSD
Résumé : International audience
BACKGROUND: There is increasing evidence that reproductive abnormalities are increasing in frequency in both human population and among wild fauna. This increase is probably related to exposure to toxic contaminants in the environment. The use of sentinel species to raise alarms relating to human reproductive health has been strongly recommended. However, no simultaneous studies at the same site have been carried out in recent decades to evaluate the utility of wild animals for monitoring human reproductive disorders. We carried out a joint study in Guadeloupe assessing the reproductive function of workers exposed to pesticides in banana plantations and of male wild rats living in these plantations. METHODS: A cross-sectional study was performed to assess semen quality and reproductive hormones in banana workers and in men working in non-agricultural sectors. These reproductive parameters were also assessed in wild rats captured in the plantations and were compared with those in rats from areas not directly polluted by humans. RESULTS: No significant difference in sperm characteristics and/or hormones was found between workers exposed and not exposed to pesticide. By contrast, rats captured in the banana plantations had lower testosterone levels and gonadosomatic indices than control rats. CONCLUSION: Wild rats seem to be more sensitive than humans to the effects of pesticide exposure on reproductive health. We conclude that the concept of sentinel species must be carefully validated as the actual nature of exposure may varies between human and wild species as well as the vulnerable time period of exposure and various ecological factors.
Environmental health : a global access science source [electronic resource]

hal-00669505
https://hal.archives-ouvertes.fr/hal-00669505
DOI : 10.1186/1476-069X-7-40
PUBMED : 18667078

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : We conducted a rigorous test of tropical tree seedling microhabitat differentiation by examining microhabitat associations, survival and growth of established seedlings of ten tropical tree species representing a four-factor gradient in seed size. Eight microhabitat variables describing soil and light conditions were measured directly adjacent to each of 588 seedlings within twelve 10×100 m belt transects at Paracou, French Guiana, and at 264 reference points along the transects. From these measurements, we defined three principal components describing soil richness, soil softness and canopy openness. Six of ten species (in 9 of 30 total cases) were distributed non-randomly with respect to microhabitat along at least one principal component. However, few species demonstrated clear microhabitat specialization. All shifts in distribution relative to reference points were in the same direction (richer, softer soil). Furthermore, of 135 pairwise comparisons among the species, only 7 were significantly different. More than three-fourths of all seedlings (75.3%) survived over the 2-year monitoring period, but survival rates varied widely among species. In no case was the probability of survival influenced by any microhabitat parameter. Relative height growth rates for the seedlings over 2 years varied from -0.031 cm cm-1 year-1 (Dicorynia guianensis, Caesalpiniaceae) to 0.088 cm cm-1 year-1 (Virola michelii, Myristicaceae). In only 4 of 30 cases was height growth significantly associated with one of the three principal components. Because the conditions in this study were designed to maximize the chance of finding microhabitat differentiation among a group of species differing greatly in life history traits, the lack of microhabitat specialization it uncovered suggests that microhabitat partitioning among tropical tree species at the established seedling stage is unlikely to contribute greatly to coexistence among these species.
ISSN: 0029-8549

hal-01031928
https://hal.archives-ouvertes.fr/hal-01031928
DOI : 10.1007/s00442-004-1691-3

Éditeur(s) : HAL CCSD
Résumé : International audience
The Chromosome-Centric Human Proteome Project (C-HPP) aims to identify "missing" proteins in the neXtProt knowledgebase. We present an in-depth proteomics analysis of the human sperm proteome to identify testis-enriched missing proteins. Using protein extraction procedures and LC-MS/MS analysis, we detected 235 proteins (PE2-PE4) for which no previous evidence of protein expression was annotated. Through LC-MS/MS and LC-PRM analysis, data mining, and immunohistochemistry, we confirmed the expression of 206 missing proteins (PE2-PE4) in line with current HPP guidelines (version 2.0). Parallel reaction monitoring acquisition and sythetic heavy labeled peptides targeted 36 ≪one-hit wonder≫ candidates selected based on prior peptide spectrum match assessment. 24 were validated with additional predicted and specifically targeted peptides. Evidence was found for 16 more missing proteins using immunohistochemistry on human testis sections. The expression pattern for some of these proteins was specific to the testis, and they could possibly be valuable markers with fertility assessment applications. Strong evidence was also found of four "uncertain" proteins (PE5); their status should be re-examined. We show how using a range of sample preparation techniques combined with MS-based analysis, expert knowledge, and complementary antibody-based techniques can produce data of interest to the community. All MS/MS data are available via ProteomeXchange under identifier PXD003947. In addition to contributing to the C-HPP, we hope these data will stimulate continued exploration of the sperm proteome. © 2016 American Chemical Society.
Journal of Proteome Research

hal-01406074
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01406074
DOI : 10.1021/acs.jproteome.6b00400
PUBMED : 27444420