Éditeur(s) : HAL CCSD
Résumé : International audience
Acute viral hepatitis results from an inefficient innate immune response to clear the virus and a delayed immune adaptive response. Murine hepatitis virus (MHV) infection represents a unique animal model to identify new escape mechanisms in liver of innate immune responses. The objective of this study was to identify early disorders in TLRs, helicases, cytokines and chemokines favoring the development of a fulminant hepatitis. Groups of C57BL/6 WT and TLR2-/- mice were infected with highly hepatotropic MHV3 and/or weakly hepatotropic MHV-A59 viruses. Histopathological analysis of liver and mRNA expression levels of viral nucleoprotein, viral sensors, interferons, cytokines and chemokines assessed by RT-qPCR were done in the first 3 days of infection. The results showed that liver damages, viral replication and mRNA levels of TLR-2, TLR-3, RIG-1, MDA-5, IL-33, IFN-b, CXCL1, CXCL9, CXCL-10, CXCL-11, CCL3, CCL5, IL-6 and TNF-a increased higher or appeared sooner in MHV3-infected WT than in MHV-A59 and TLR2-/- mice. To address the role of hepatocytes in TLR2-dependent viral replication and innate immune factors, in vitro viral infections were performed on FL83B cells. The results showed that viral replication and mRNA levels of TLR-2 and IL-6 occurred sooner than those of other innate immune parameters. Moreover, blockade of TLR-2 by siRNA decreased IFN-b, TNF-a, CXCL-1, CXCL-10 and CCL-2 expression in infected hepatocytes and also inhibited the viral replication of MHV-A59, and at a lesser extent of MHV3, suggesting that TLR-2 signaling promotes simultaneously the viral replication and the production of innate immune factors in hepatocytes, exacerbating viral hepatitis.
Frontiers of Immunology
Milan, Italy

hal-01118155
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01118155
DOI : 10.3389/conf.fimmu.2013.02.00648

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Type I and Type II eclogite xenoliths from the Roberts Victor kimberlite (South Africa) show marked differences in terms of microstructures, mineralogy, major-and trace-element compositions and oxygen-isotope compositions. The unequilibrated microstructures of Type I eclogites, their typical accessory assemblages (phologopite, diamond, sulphides, fluid inclusions) and the ubiquitous presence of "melt pockets" in garnets provide strong evidence of metasomatism. Type II eclogites systematically lack such features and are microstructurally more equilibrated. Type I eclogites are more magnesium-rich than most Type II (mean Mg# = 0.56 vs. 0.46), while Type II eclogites are generally more Ca-rich (mean CaO = 9 vs. 12 wt%) and Fe-rich (mean FeO = 10 vs. 12 wt%). Type I eclogites are systematically enriched in LREE, Sr, Ba, alkali elements, HFSE, Th and U compared to the more depleted Type II eclogites. Calculated trace-element patterns of fluids in equilibrium with Type I eclogites are closely similar to those of volatile-rich small-volume mantle melts in the carbonatite-kimberlite spectrum commonly inferred to be responsible for mantle metasomatism. Although oxygen isotopes are often used to argue for a subduction origin of mantle eclogites, correlations between delta O-18 of garnet and typical metasomatic tracers suggest that the metasomatic process also has shifted the oxygen-isotope compositions of the Type I eclogites toward heavier values. Roberts Victor Type I eclogites thus carry the imprint of a metasomatic process that strongly modified their major-element, trace-element and isotopic compositions, while the more pristine Type II eclogites escaped this modification. Therefore, attempts to constrain the origin of Roberts Victor eclogites should not be based on the much more abundant Type I eclogites, which retain little geochemical memory of their protoliths. The most suitable materials for such investigations may be the less metasomatised, but more rare, Type II eclogites.
ISSN: 0016-7037

hal-00669867
https://hal.archives-ouvertes.fr/hal-00669867
DOI : 10.1016/j.gca.2011.08.035

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Large sand intrusions often exhibit conical morphologies analogous to magmatic intrusions such as saucer-shaped or cup-shaped sills. Whereas some physical processes may be similar, we show with scaled experiments that the formation of conical sand intrusions may be favoured by the pore-pressure gradients prevailing in the host rock before sand injection. Our experiments involve injecting air into a permeable and cohesive analogue material to produce hydraulic fractures while controlling the pore pressure field. We control the state of overpressure in the overburden by applying homogeneous basal pore pressure, and then adding a second local pore pressure field by injecting air via a central injector to initiate hydraulic fractures near the injection point. In experiments involving small vertical effective stresses (small overburden, or high pore fluid overpressure), the fracturing pressure (lambda(fract)) is supralithostatic and two dipping fractures are initiated at the injection point forming a conical structure. From theoretical considerations, we predict that high values of lambda(fract), are due to strong cohesion or high pore fluid overpressure distributed in the overburden. Such conditions are favoured by the pore pressure/stress coupling induced by both pore pressure fields. The dips of cones can be accounted for elastic-stress rotation occurring around the source. Contrary to magmatic chamber models, the aqueous fluid overpressure developed in a parent sandbody (and prevailing before the formation of injectites) may diffuse into the surrounding overburden, thus favouring stress rotation and the formation of inclined sheets far from the parent source. For experiments involving higher vertical effective stresses (thick overburden or low pore fluid overpressure), the fracturing pressure is lower than the lithostatic stress, and a single fracture is opened in mode I which then grows vertically. At a critical depth, the fracture separates into two dilatant branches forming a flat cone. We make use of a P.I.V. (Particle Imaging Velocimetiy) technique to analyse plastic deformation, showing that these inclined fractures are opened in mixed modes. Close to the surface, they change into steep shear bands where fluids can infiltrate. The final morphology of the fracture network is very similar to the common tripartite architecture of various injection complexes, indicating that different mechanisms may be involved in the formation of dykes. Feeder dykes under the sill zones may open as tensile fractures, while overlying dykes may be guided by the deformation induced by the growth of sills. These deformation conditions may also favour the formation of fluid escape structures and pockmarks. (C) 2011 Elsevier B.V. All rights reserved.
ISSN: 0012-821X

hal-00745640
https://hal.archives-ouvertes.fr/hal-00745640
DOI : 10.1016/j.epsl.2011.10.029

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
BACKGROUND AND AIMS: Infection by hepatitis C virus is a worldwide health problem. An inadequate Th2 cytokine response promotes the fibrosis-cirrhosis fate. Immune-modulating molecules favoring a Th2 profile, such as HLA-G molecules of the HLA class Ib family, may play a role in chronic hepatitis. HLA-G contributes to the escape of tumors, and their involvement in viral infections has been increasingly described. The aim of this work was to study the expression of HLA-G in the liver, its cellular source and its regulation in cases of chronic C hepatitis. METHODS: HLA-G cells in blocks of liver derived from patients infected with HCV were labeled by immunohistochemistry and enumerated. Double immunofluorescence allowed the identification of the cellular source. HLA-G secretion by a human mast cell line was quantified by ELISA after various stimulations. After treatment with IFN-α real-time PCR was performed to determine the kinetics of cytokine expression profiles, followed by heat map clustering analysis. RESULTS: The number of HLA-G + cells was significantly associated with the area of fibrosis. For the first time, we identify the HLA-G+ cells as being mast cells. HLA-G secretion was significantly induced in human mast cells stimulated by IL-10 or interferons of class I. The transcriptome of the secretome of this cell line stimulated by IFN-α revealed that i) the HLA-G gene is upregulated late, ii) T lymphocytes and NK cells are recruited. CONCLUSIONS: These findings suggest an autocrine loop in the genesis of HCV liver fibrosis, based on mast cells expressing HLA-G.
ISSN: 0168-8278

hal-00874106
https://hal.archives-ouvertes.fr/hal-00874106
https://hal.archives-ouvertes.fr/hal-00874106/document
https://hal.archives-ouvertes.fr/hal-00874106/file/Amiot-Expression-HLA-G-revised.pdf
DOI : 10.1016/j.jhep.2013.09.006

Éditeur(s) : HAL CCSD
Résumé : Some States host Open Registers (also known under the pejorative term of “flags of convenience”): ships are registered regardless of their actual owners’ nationality or residence, and thus States enable them to escape from their country of origin’s legal framework. By being an aspect of globalization, this phenomenon has been growing up for 30 years, and is nowadays widespread, or even universal. Because each State fixes the conditions for implementation of its right to register ships, the phenomenon is a legal one. But it has strong economic implications too. Indeed, the laws of the State of registration – namely “flag state” – determine certain costs or some advantages for the company that adopts it, and can be attractive or repellent as for them.This work proposes an economic reading of the open registers of ships as a recent object of economics and tries to highlight the contribution of this activity to small islands’ economies, especially through some Caribbean examples (the Caribbean is by the way the historical cradle of this activity and offshore financial centres).Based on data concerning the last 30 years and 7 open-registry countries of the Caribbean (Gross Domestic Products and some items of the balances of payments), the research conducted here shows that, except perhaps Antigua, no significant correlation exists in host countries between the fleets registered and the economic gains : the “open registration” is not, by itself, a gain for small island economies surveyed.
Certains Etats pratiquent la libre immatriculation, également connue sous le terme péjoratif de « pavillons de complaisance » : ils enregistrent des navires chez eux sans considération de la nationalité ou de la résidence effective des propriétaires, et leur permettent ainsi d’échapper au cadre légal de leur pays d’origine. Aspect de la mondialisation, le phénomène qui a pris de l’ampleur dans les 30 dernières années est aujourd’hui largement répandu, voire universel.Au-delà des aspects juridiques (chaque Etat organise son droit à immatriculer les navires), la libre immatriculation a des conséquences économiques fortes car la législation de l’Etat d’immatriculation – dit « Etat du pavillon » – détermine certains coûts ou certaines facilités pour l’entreprise qui l’adopte.Le présent travail propose une lecture de la libre immatriculation des navires en tant qu’objet récent de la science économique et tente, à ce titre, de lever le voile sur la portée de cette activité sur les pays d’accueil, en prenant le cadre restreint de petites économies insulaires de la Caraïbe, berceau historique de cette activité et des centres financiers offshore.Sur la base de données économiques sur 30 ans concernant 7 pays de libre immatriculation de la Caraïbe (produits intérieurs bruts et éléments des balances des paiements), la recherche menée montre qu’hormis peut-être le cas à approfondir d’Antigua, aucune corrélation significative n’existe entre le nombre de navires immatriculés et les gains économiques dans les pays d’accueil. Ces résultats vont dans le sens des critiques de la libre immatriculation : cette activité ne présente pas, en soi, un gain pour les petites économies insulaires étudiées.
https://tel.archives-ouvertes.fr/tel-00697681

Droits : info:eu-repo/semantics/OpenAccess
NNT : 2012AGUY0508
tel-00697681
https://tel.archives-ouvertes.fr/tel-00697681
https://tel.archives-ouvertes.fr/tel-00697681/document
https://tel.archives-ouvertes.fr/tel-00697681/file/12angelleli.pdf

Résumé : Qu’est ce qui explique la faiblesse d’un Etat qui s’est engagé depuis plus de vingt ans à rompre avec les pratiques de l’autoritarisme et du néo-patrimonialisme ? Pourquoi les promesses d’une démocratisation réussie, malgré le soutien massif des acteurs de la « société internationale » ont paradoxalement débouché sur la déstabilisation de l’Etat dans un contexte de crises récurrentes ? Ce sont les interrogations principales qui orientent cette recherche qui se donne pour ambition d’étudier les processus par lesquels les élites et les masses haïtiennes s’approprient la démocratie. De ce point de vue, la démocratisation s’analyse comme une ressource politique et institutionnelle revendiquée par ces dernières comme élément probant de leur adhésion à la grammaire démocratique. Dès lors, il s’agit de scruter les routes sinueuses et chaotiques empruntées par la démocratisation en Haïti, en interrogeant les diverses séquences marquantes de l’histoire politique haïtienne. L’accent est mis sur l’importation des technologies institutionnelles d’origine occidentale, notamment durant la période ouverte en 1994 avec la réinstallation de Jean-Bertrand Aristide dans ses fonctions de président et celle qui commence en 2004 avec son départ forcé du pouvoir. Ces deux séquences historiques, sous-tendues par des plans de reconstruction de l’Etat, sont marquées par la mobilisation de ressources diverses, en vue de jeter les bases d’une nouvelle gouvernance politique et économique. Elles circonscrivent également un champ de représentations conflictuelles, caractérisé par la compétition entre les Etats occidentaux et la lutte pour la répartition des enjeux de puissance entre les organisations internationales et les élites locales. L’analyse du processus de démocratisation conduit à des observations paradoxales : l’exacerbation de la violence, l’actualisation de l’autoritarisme, la multiplication des situations déviantes découlant des stratégies des acteurs locaux. Ces derniers tentent d’échapper aux contraintes imposées par des acteurs externes qui interviennent afin de sanctionner, d’arbitrer et d’imposer des décisions aux factions en luttes pour le pouvoir. Ainsi, la démocratisation passe par des crises d’adaptation, des phases contradictoires de recomposition, d’hybridation des institutions importées et parfois de contournement des normes et procédures. Entre les acteurs internes et externes s’établit et s’organise une dynamique interactive qui influence l’Etat dont la configuration finale est loin de correspondre aux objectifs affichés à travers les réformes engagées. Afin de mieux mettre en évidence les traits distinctifs de cet Etat forgé dans l’incertitude et l’imprécision, la thèse propose de restituer les dynamiques et l’historicité propres à la société haïtienne, en privilégiant l’hypothèse de la réinvention de l’Etat. Paradoxalement, l’État peut être le produit de processus de violence et la part chaotique que comporte la situation en Haïti peut recéler les prémices de sa construction.
What does explain the weakness of a State which has committed more than 20 years to break with the practices of authoritarianism and neo-patrimonialism? Why the promises of a successful democratization, despite the massive support of the actors of the “international society” paradoxically led to the destabilization of the State in the context of recurrent crises? These are the key questions guiding this research, whose ambition is to study the processes by which elites and the Haitian masses appropriate democracy. From this point of view, democratization is analyzed as a political and institutional resource claimed by them as convincing evidence of their adherence to democratic grammar. Therefore, it is scrutinizing the winding roads and chaotic way borrowed by democratization in Haiti, by querying various striking sequences of Haitian political history. Emphasis is placed on the importation of institutional technologies of Western origin, particularly during the period opened in 1994 with the resettlement of Jean-Bertrand Aristide in his duties as president and on the period which starts in 2004 with his forced departure from power. These two historical sequences, underpinned by plans for the reconstruction of the State, are marked by the mobilization of various resources, with a view to laying the foundations for new political and economic governance. They also define a field of conflicting representations, characterized by competition between Western States and the struggle for the distribution of power between the international organizations and local elites issues.The analysis of the process of democratization leads to paradoxical representations: the exacerbation of violence, the updating of authoritarianism, the multiplication of deviant situations arising from the strategies of local actors. The latter are trying to escape from the constraints imposed by external actors involved in order to punish, to arbitrate and to impose decisions on the factions struggling for power. Thus, democratization goes hand in hand with crises of adaptation, contradictory phases of re-composition, hybridization of the imported institutions and, sometimes with circumvention of the standards and procedures. Between internal and external actors are settled and organized interactive dynamics that influence the State of which the final configuration is far short of the objectives set out through the aimed reforms. In order to better highlight the distinguishing features of this State forged in uncertainty and vagueness, the thesis proposes to analyze the dynamics and historicity of Haitian society, privileging, the hypothesis of the reinvention of the State. Paradoxically, the State may be the product of process of violence and the chaotic part that includes the situation in Haiti can conceal the beginning of its construction.

http://www.theses.fr/2014AGUY0849/document

Résumé : Certains Etats pratiquent la libre immatriculation, également connue sous le terme péjoratif de « pavillons de complaisance » : ils enregistrent des navires chez eux sans considération de la nationalité ou de la résidence effective des propriétaires, et leur permettent ainsi d’échapper au cadre légal de leur pays d’origine. Aspect de la mondialisation, le phénomène qui a pris de l’ampleur dans les 30 dernières années est aujourd’hui largement répandu, voire universel.Au-delà des aspects juridiques (chaque Etat organise son droit à immatriculer les navires), la libre immatriculation a des conséquences économiques fortes car la législation de l’Etat d’immatriculation – dit « Etat du pavillon » – détermine certains coûts ou certaines facilités pour l’entreprise qui l’adopte.Le présent travail propose une lecture de la libre immatriculation des navires en tant qu’objet récent de la science économique et tente, à ce titre, de lever le voile sur la portée de cette activité sur les pays d’accueil, en prenant le cadre restreint de petites économies insulaires de la Caraïbe, berceau historique de cette activité et des centres financiers offshore.Sur la base de données économiques sur 30 ans concernant 7 pays de libre immatriculation de la Caraïbe (produits intérieurs bruts et éléments des balances des paiements), la recherche menée montre qu’hormis peut-être le cas à approfondir d’Antigua, aucune corrélation significative n’existe entre le nombre de navires immatriculés et les gains économiques dans les pays d’accueil. Ces résultats vont dans le sens des critiques de la libre immatriculation : cette activité ne présente pas, en soi, un gain pour les petites économies insulaires étudiées.
Some States host Open Registers (also known under the pejorative term of “flags of convenience”): ships are registered regardless of their actual owners’ nationality or residence, and thus States enable them to escape from their country of origin’s legal framework. By being an aspect of globalization, this phenomenon has been growing up for 30 years, and is nowadays widespread, or even universal. Because each State fixes the conditions for implementation of its right to register ships, the phenomenon is a legal one. But it has strong economic implications too. Indeed, the laws of the State of registration – namely “flag state” – determine certain costs or some advantages for the company that adopts it, and can be attractive or repellent as for them.This work proposes an economic reading of the open registers of ships as a recent object of economics and tries to highlight the contribution of this activity to small islands’ economies, especially through some Caribbean examples (the Caribbean is by the way the historical cradle of this activity and offshore financial centres).Based on data concerning the last 30 years and 7 open-registry countries of the Caribbean (Gross Domestic Products and some items of the balances of payments), the research conducted here shows that, except perhaps Antigua, no significant correlation exists in host countries between the fleets registered and the economic gains : the “open registration” is not, by itself, a gain for small island economies surveyed.

http://www.theses.fr/2012AGUY0508/document