Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD Elsevier Masson
Résumé : International audience
The diagnosis of diabetic foot infections is difficult due to limitations of conventional culture-based techniques. The objective of this study was to evaluate the contribution of denaturing gradient gel electrophoresis (DGGE) in the microbiological diagnosis of diabetic foot ulcers in comparison to conventional techniques, and also to evaluate the need to perform a biopsy sample for this diagnosis. Twenty diabetic patients (types 1 and 2) with foot ulcers (grades 1-4) were included. After debridement of their wounds, samples were taken in duplicate by surface swabbing and deep-tissue biopsy. The samples were analyzed by conventional culture and by a new molecular biology tool, DGGE technology. Polymerase chain reaction (PCR)-DGGE led to the identification of more bacteria than did conventional cultures (mean: 2.35 vs 0.80, respectively). In 11 cases, the technology detected pathogenic species not isolated by classical cultures. PCR-DGGE also identified significantly more pathogenic species at deep levels compared with species detected at superficial levels (87% vs 58%, respectively; P = 0.03). In 9/20 cases, pathogenic bacteria were detected only in deep samples, revealing the need to perform tissue biopsy sampling. DGGE, achievable in 48h, could be a useful technique for the bacteriological diagnosis of diabetic foot infections. It may help to identify pathogenic bacteria in deeply infected ulcers, thereby contributing to a more appropriate use of antibiotics.
ISSN: 1262-3636

hal-01122240
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122240
DOI : 10.1016/j.diabet.2014.03.002
PUBMED : 24751989

Éditeur(s) : HAL CCSD Cerimes
Résumé : L'ensemble des articles est archivé sur ArchiveTémaTice. Les actes sont aussi accessibles sur le site du colloque http://www.cerimes.fr/colloquefrancomexicain
Les textes qui composent ces actes ont été présentés à l'occasion du premier colloque franco-mexicain en sciences de la communication, organisé par la Asociación mexicana en Ciencias de la comunicación (AMIC) et la Société Française des Sciences de l'Information et de la Communication (SFSIC), à Mexico, du 8 au 10 avril 2002.

Les médias classiques: radio, télévision (8 avril 2002)
Introduction . La coopération scientifique bilatérale et l'interculturel dans l'activité de recherche, Bruno Ollivier
La recherche française sur le média et les discours télévisuels: éléments pour un bilan prospectif, Guy Lochard
Parcours de la programmation télévisée au Mexique, Florence Toussaint
El regimen de la television hertziana en Mexico, Claudia I. Garcia Rubio
La question de l'éthos et du genre en communication politique, Simone Bonnafous
L'exposition de la vie privée dans les médias, André Vitalis

Identités, culture, communication (9 avril 2002)
Reconfiguraciones cimunicativas de la socialidad y reencantamientos de la communication identidad, Jesus Martin-Barbero
Identité, culture et communication, Jean François Tetu
Las organizaciones civiles en las radiodifusoras comerciales, Dr. José Cisneros E.
Les SIC et l'altérité: démarches d'une recherche sur la Chine. réflexions épitstémologiques sur les analyses de corpus à base communicationnelles de langue et de culture non-maternelles, Dominique Colomb
Transicion, reforma del estado y medios de comunicacion en Mexico, Dr. Javier Esteinou Madrid
Medios masivos y formacion psicosocial, Enrique Guinsberg

Technologies d'information et de communication ( 10 avril 2002)
Quoi de nouveau dans la recherche française en informatique et communication ? Bernard Miege
Convergencia tecnologica: perspectivas de investigacion en Mexico, Delia Crovi Druetta
Medias, technologies et public: la notion de projet au cœur d'une éventuelle médiation, Claire Noy
La formacion de profesores de educacion superior en el uso de la computadora y la internet. reflexioànes sobre el tema, Luz Maria Garay Cruz
Vers un retour à une lecture non linéaire, Schmidt C. T, Roxin I.(), Mercier D, Hufschmitt B. & Cotten J.-P
NTIC et éducation à distance. Le cas d'Edusat au Mexique face au cas français, Norma Veronica Fernandez Arriaga.
Les sciences de l'éducation et les sciences de la communication en dialogue: à propos des médias et des technologies éducatives, Geneviève Jacquinot-Delaunay
Internet et Intranet dans les organisations "un scénario récurrent", Jean-Antoine Corbalan
De l'université virtuelle au campus numérique : simple effet de traduction ou changement de paradigme, Françoise Thibault
Competencias mediacionales de los sujetos en programas educativos en entornos digitales, Maria Elena Chan Nunez
Le journal quotidien sur le Web. L'identité éditoriale au risque du formatage, Annelise Touboul
Conclusion, Carmen Gomez-Mont
Premier colloque franco-mexicain de sciences de la communication
Mexico, France

Droits : info:eu-repo/semantics/OpenAccess
ISBN : http://www.cerimes.education.fr
edutice-00000362
https://edutice.archives-ouvertes.fr/edutice-00000362
https://edutice.archives-ouvertes.fr/edutice-00000362/document
https://edutice.archives-ouvertes.fr/edutice-00000362/file/actes.pdf

Éditeur(s) : Springer
Résumé : Series of sensitivity tests were performed with a z-coordinate, global eddy-permitting (1/4 degrees) ocean/sea-ice model (the ORCA-R025 model configuration developed for the DRAKKAR project) to carefully evaluate the impact of recent state-of-the-art numerical schemes on model solutions. The combination of an energy-enstrophy conserving (EEN) scheme for momentum advection with a partial step (PS) representation of the bottom topography yields significant improvements in the mean circulation. Well known biases in the representation of western boundary currents, such as in the Atlantic the detachment of the Gulf Stream, the path of the North Atlantic Current, the location of the Confluence, and the strength of the Zapiola Eddy in the south Atlantic, are partly corrected. Similar improvements are found in the Pacific, Indian, and Southern Oceans, and characteristics of the mean flow are generally much closer to observations. Comparisons with other state-of-the-art models show that the ORCA-R025 configuration generally performs better at similar resolution. In addition, the model solution is often comparable to solutions obtained at 1/6 or 1/10 degrees resolution in some aspects concerning mean flow patterns and distribution of eddy kinetic energy. Although the reasons for these improvements are not analyzed in detail in this paper, evidence is shown that the combination of EEN with PS reduces numerical noise near the bottom, which is likely to affect current-topography interactions in a systematic way. We conclude that significant corrections of the mean biases presently seen in general circulation model solutions at eddy-permitting resolution can still be expected from the development of numerical methods, which represent an alternative to increasing resolution.
Ocean Dynamics (1616-7341) (Springer), 2006-12 , Vol. 56 , N. 5-6 , P. 543-567

Droits : © Springer 2006. Part of Springer Science+Business Media
http://archimer.ifremer.fr/doc/2006/publication-3514.pdf
DOI:10.1007/s10236-006-0082-1
http://archimer.ifremer.fr/doc/00000/3514/

Éditeur(s) : HAL CCSD Wiley/Blackwell
Résumé : 4 pages
International audience
This article documents the addition of 268 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Alburnoides bipunctatus, Chamaerops humilis, Chlidonias hybrida, Cyperus papyrus, Fusarium graminearum, Loxigilla barbadensis, Macrobrachium rosenbergii, Odontesthes bonariensis, Pelteobagrus vachelli, Posidonia oceanica, Potamotrygon motoro, Rhamdia quelen, Sarotherodon melanotheron heudelotii, Sibiraea angustata, Takifugu rubripes, Tarentola mauritanica, Trimmatostroma sp. and Wallago attu. These loci were cross-tested on the following species: Alburnoides fasciatus, Alburnoides kubanicus, Alburnoides maculatus, Alburnoides ohridanus, Alburnoides prespensis, Alburnoides rossicus, Alburnoides strymonicus, Alburnoides thessalicus, Alburnoides tzanevi, Carassius carassius, Fusarium asiaticum, Leucaspius delineatus, Loxigilla noctis dominica, Pelecus cultratus, Phoenix canariensis, Potamotrygon falkneri, Trachycarpus fortune and Vimba vimba.
ISSN: 1755-098X

hal-00823716
https://hal.archives-ouvertes.fr/hal-00823716
DOI : 10.1111/1755-0998.12095
PUBMED : 23521844

Éditeur(s) : HAL CCSD
Résumé : National audience
Nous définissons l’efficacité des mouvements de tracé comme la capacité à concilier des caractéristiques de rapidité, fluidité et régularité satisfaisant aux attentes des enseignements scolaires. L’équipe éducative en charge, dès l’entrée à l’école maternelle, de l’accompagnement de l’acquisition de l’écriture, ne dispose ni de moyens d’évaluation objectifs et fiables de l’efficacité des mouvements de tracé adaptés aux profils des très jeunes enfants, ni d’outils d’aide à la décision des exercices les plus appropriés pour renforcer et contrôler la progression de chaque enfant compte-tenu de son déjà-là psychomoteur notamment. Nous expliciterons en quoi les dispositifs interactifs d’entrainement graphomoteur orientés stylo sont inadaptés à constituer des aides à la décision et à l’accompagnement des acquisitions pour ces trois domaines. Nous vérifierons ensuite expérimentalement l’existence de pistes, à la fois d’un point de vue technologique et méthodologique, pour doter ces dispositifs de capacité d’évaluation de l’efficacité des mouvements de tracé du jeune apprenti-scripteur.
Atelier IHM'A, actes IHM'14, 26e conférence francophone sur l'Interaction Homme-Machine
Lille, France

Droits : info:eu-repo/semantics/OpenAccess
hal-01089622
https://hal.archives-ouvertes.fr/hal-01089622
https://hal.archives-ouvertes.fr/hal-01089622/document
https://hal.archives-ouvertes.fr/hal-01089622/file/p29-duval.pdf

Éditeur(s) : HAL CCSD
Résumé : Best paper for innovative research
International audience
This paper investigates the interest of using the Kinematic Theory of rapid human movements to analyseand evaluate the handwriting produced by young kindergarten children in typical classroom environment. A total of66 children participated in this trial. For the preliminary results reported herein, movements from 15 children, takenevenly from three different levels (3, 4, and 5 years old), were analyzed. Our results confirm that 1) the sigmalognormalequation can model accurately childen’s movement and 2) that this modeling can differentiate betweenchildren of different school levels, hence supporting the relevance of pursuing along this original pathway.
Recent Progress in Graphonomics: Learn from the Past. IGS 2013
Nara, Japan

hal-01098125
https://hal.archives-ouvertes.fr/hal-01098125

Éditeur(s) : HAL CCSD American Chemical Society
Résumé : 8 pages
International audience
In the protected area of the Cévennes National Park (Southern France), 114 wild brown trout (Salmo trutta fario) were captured at six locations affected to different extents by historical mining and metallurgy dating from the Iron Age to Modern Times. Cadmium and lead in trout livers and muscles reflect high sediment contamination, although an age-related effect was also detected for hepatic metal concentrations. Lead isotope signatures confirm exposure to drainage from mining and metallurgical waste. Developmental instability, assessed by fluctuating asymmetry, is significantly correlated with cadmium and lead concentrations in trout tissues, suggesting that local contamination may have affected fish development. Nowadays, the area is among the least industrialized in France. However, our results show that 60% of the specimens at one site exceed EU maximum allowed cadmium or lead concentration in foodstuffs. The mining heritage should not be neglected when establishing strategies for long-term environmental management.
ISSN: 0013-936X

hal-00621255
https://hal.archives-ouvertes.fr/hal-00621255
DOI : 10.1021/es200755n
PUBMED : 21739978

Éditeur(s) : HAL CCSD Wiley
Résumé : Genetic variation is generally considered a prerequisite for adaptation to new environmental conditions. Thus the discovery of genetically depauperate but geographically widespread species is unexpected. We used 12 paternally inherited chloroplast microsatellites to estimate population genetic variation across the full range of an emblematic circum-Mediterranean conifer, stone pine (Pinus pinea L.). The same chloroplast DNA haplotype is fixed in nearly all of the 34 investigated populations. Such a low level of variation is consistent with a previous report of very low levels of diversity at nuclear loci in this species. Stone pine appears to have passed through a severe and prolonged demographic bottleneck, followed by subsequent natural- and human-mediated dispersal across the Mediterranean Basin. No other abundant and widespread plant species has as little genetic diversity as P. pinea at both chloroplast and nuclear markers. However, the species harbors a nonnegligible amount of variation at adaptive traits. Thus a causal relationship between genetic diversity, as measured by marker loci, and the evolutionary precariousness of a species, cannot be taken for granted
ISSN: 0014-3820

hal-01032062
https://hal.archives-ouvertes.fr/hal-01032062
DOI : 10.1111/j.1558-5646.2007.00294.x