Éditeur(s) : Santiago de los Caballeros : Pontificia Universidad Católica Madre y Maestra Santiago de los Caballeros : Pontificia Universidad Católica Madre y Maestra
Résumé : (Funding) Digitized with funding from the Digital Library of the Caribbean grant awarded by TICFIA.
República Dominicana

Droits : All rights reserved by the source institution.
RD 303.69 N972e

Éditeur(s) : HAL CCSD Springer Verlag (Germany)
Résumé : International audience
Mount Pinatubo volcano erupted in June 1991 in the main island of Luzon belonging to the Philippines archipelago. Huge economic losses and population exodus have followed. This major crisis has been relayed with other crises due to rainfed lahars which have been supplied with eruption deposits. These lahars have occurred every year since 1991 during the rainy season. They will probably last until 2005. After a brief presentation of the Philippine official response system to disasters, this paper draws up a critical analysis of the different kinds of institutional and social responses deployed to manage the different crisis and post-crisis phases of this event. Based on three viewpoints: from population, media and other actors, this analysis attempts to point out the strengths and weaknesses of the official management system, especially by studying the efficiency and the range of the solutions taken. So, it appears that the management of the June 1991 main crisis (eruption) was a success. On the other hand, difficulties have occurred with lahars risk management. Indeed, these lahars have obliged the authorities to protect and relocate thousands of people. In spite of persistent problems, the management system (monitoring/warning/evacuation) of lahar crises improves year after year. Failures appear especially within the rehabilitation program (protection/rehousing). Many direct (lack of means, preparedness, coordination, dialog,
etc.) and indirect (politico-administrative, socio-economic, cultural contexts) factors come together to lock the wheels of
the institutional response system. They defer the socio-economic start of this vital northern Philippines area.
GeoJournal

halshs-00119436
https://halshs.archives-ouvertes.fr/halshs-00119436
https://halshs.archives-ouvertes.fr/halshs-00119436/document

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Mammalian spermatogenesis, which takes place in complex testicular structures called seminiferous tubules, is a highly specialized process controlled by the integration of juxtacrine, paracrine and endocrine information. Within the seminiferous tubules, the germ cells and Sertoli cells are surrounded by testicular fluid (TF), which probably contains most of the secreted proteins involved in crosstalk between these cells. It has already been established that germ cells can modulate somatic Sertoli cell function through the secretion of diffusible factors. We studied the germ cell secretome, which was previously considered inaccessible, by analyzing the TF collected by microsurgery in an "integrative omics" strategy combining proteomics, transcriptomics, genomics and interactomics data. This approach identified a set of proteins preferentially secreted by Sertoli cells or germ cells. An interaction network analysis revealed complex, interlaced cell-cell dialog between the secretome and membranome of seminiferous cells, mediated via the TF. We then focused on germ cell-secreted candidate proteins, and we identified several potential interacting partners located on the surface of Sertoli cells. Two interactions, APOH/CDC42 and APP/NGFR, were validated in situ, in a proximity ligation assay (PLA). Our results provide new insight into the crosstalk between germ cells and Sertoli cells occurring during spermatogenesis. Our findings also demonstrate that this "integrative omics" strategy is powerful enough for data mining and highlighting meaningful cell-cell communication events between different types of cells in a complex tissue, via a biological fluid. This integrative strategy could be applied more widely, to gain access to secretomes that have proved difficult to study whilst avoiding the limitations of in vitro culture.
ISSN: 1932-6203

hal-01129957
https://hal.archives-ouvertes.fr/hal-01129957
https://hal.archives-ouvertes.fr/hal-01129957/document
https://hal.archives-ouvertes.fr/hal-01129957/file/2014_FChalmel_PlosOne_%7B1236BFCE-4830-400D-B816-4B6651311900%7D.pdf
DOI : 10.1371/journal.pone.0104418

Éditeur(s) : HAL CCSD Public Library of Science
Résumé : International audience
Mammalian spermatogenesis, which takes place in complex testicular structures called seminiferous tubules, is a highly specialized process controlled by the integration of juxtacrine, paracrine and endocrine information. Within the seminiferous tubules, the germ cells and Sertoli cells are surrounded by testicular fluid (TF), which probably contains most of the secreted proteins involved in crosstalk between these cells. It has already been established that germ cells can modulate somatic Sertoli cell function through the secretion of diffusible factors. We studied the germ cell secretome, which was previously considered inaccessible, by analyzing the TF collected by microsurgery in an "integrative omics" strategy combining proteomics, transcriptomics, genomics and interactomics data. This approach identified a set of proteins preferentially secreted by Sertoli cells or germ cells. An interaction network analysis revealed complex, interlaced cell-cell dialog between the secretome and membranome of seminiferous cells, mediated via the TF. We then focused on germ cell-secreted candidate proteins, and we identified several potential interacting partners located on the surface of Sertoli cells. Two interactions, APOH/CDC42 and APP/NGFR, were validated in situ, in a proximity ligation assay (PLA). Our results provide new insight into the crosstalk between germ cells and Sertoli cells occurring during spermatogenesis. Our findings also demonstrate that this "integrative omics" strategy is powerful enough for data mining and highlighting meaningful cell-cell communication events between different types of cells in a complex tissue, via a biological fluid. This integrative strategy could be applied more widely, to gain access to secretomes that have proved difficult to study whilst avoiding the limitations of in vitro culture.
PLoS ONE

hal-01063901
https://hal.archives-ouvertes.fr/hal-01063901
DOI : 10.1371/journal.pone.0104418
PUBMED : 25111155

Éditeur(s) : Université des Antilles Études caribéennes
Résumé : Ce numéro met en perspective l’approche développementaliste et la dimension dialogique du tourisme. Les auteurs successifs y interrogent l’équation tourisme, culture et développement, par le prisme de la patrimonialisation.L’émergence puis le développement, dans les années soixante-dix, d’un tourisme culturel ont largement contribué à favoriser la prise de conscience des populations et des politiques, ainsi qu’à formaliser la nécessité d’une nouvelle approche des discours s’agissant des espaces naturels et du patrimoine historique et immatériel. Des regards croisés de juristes, d’anthropologues et de géographes, se dégage une problématique qui fait écho à un constat largement partagé : les connaissances sur les applications et les instruments des orientations politiques internationales, de même que sur leurs liens avec les identités sociales et ethniques, restent insuffisantes.Les enjeux de la patrimonialisation requièrent donc que l’on se pose, dans le domaine considéré, la question de la relation entre les concepts, les discours et les politiques, et que l’on esquisse, à partir des expériences rapportées, les contours de réponses susceptibles d’y être apportées.
This issue puts into perspective the development-centric vision and the dialogic dimension of tourism. The successive authors examine the relationship between tourism, culture, and development, in the light of heritage.In the 1970s, the emergence and the growth of cultural tourism contributed significantly to raise awareness among ordinary people and politicians, as well as to formalize the need for a new approach to discourse about natural spaces and historical and intangible heritage. The overlapping perspectives of legal experts, anthropologists, and geographers, draw out a set of problems that echoes an observation shared by many: the knowledge on the applications and the instruments of international policy guidance, as well as the knowledge on their relationship with social and ethnic identity, is still inadequate.hen dealing with the issues at stake in the heritage field, it is thus necessary to question the relationship between concepts, discourse, and policies, and to outline the basis of possible solutions from the experience covered.

Droits : info:eu-repo/semantics/openAccess
http://journals.openedition.org/etudescaribeennes/5711