Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Wood debris are an important component of mangrove marine environments. Current knowledge of the ecological role of wood falls is limited by the absence of information on metazoan colonization processes over time. The aim of this study was to provide insights to their temporal dynamics of wood eukaryotic colonization from a shallow water experiment in a mangrove swamp. Combined in situ chemical monitoring and biological surveys revealed that the succession of colonizers in the mangrove swamp relates with the rapid evolution of sulfide concentration on the wood surface. Sulfide-tolerant species are among the first colonizers and dominate over several weeks when the sulfide content is at its maximum, followed by less tolerant opportunistic species when sulfide decreases. This study supports the idea that woody debris can sustain chemosynthetic symbioses over short time-scale in tropical shallow waters.
ISSN: 0141-1136

hal-01545913
https://hal.archives-ouvertes.fr/hal-01545913
DOI : 10.1016/j.marenvres.2013.03.007

Éditeur(s) : HAL CCSD Royal Society, The
Résumé : International audience
Bathymodiolin mussels occur at hydrothermal vents and cold seeps, where they thrive thanks to symbiotic associations with chemotrophic bacteria. Closely related genera Idas and Adipicola are associated with organic falls, ecosystems that have been suggested as potential evolutionary 'stepping stones' in the colonization of deeper and more sulphide-rich environments. Such a scenario should result from specializations to given environments from species with larger ecological niches. This study provides molecular-based evidence for the existence of two mussel species found both on sunken wood and bones. Each species specifically harbours one bacterial phylotype corresponding to thioautotrophic bacteria related to other bathymodiolin symbionts. Phylogenetic patterns between hosts and symbionts are partially congruent. However, active endocytosis and occurrences of minor symbiont lineages within species which are not their usual host suggest an environmental or horizontal rather than strictly vertical transmission of symbionts. Although the bacteria are close relatives, their localization is intracellular in one mussel species and extracellular in the other, suggesting that habitat choice is independent of the symbiont localization. The variation of bacterial densities in host tissues is related to the substrate on which specimens were sampled and could explain the abilities of host species to adapt to various substrates.
ISSN: 0950-1193

hal-00755293
https://hal.univ-antilles.fr/hal-00755293
DOI : 10.1098/rspb.2008.1101

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Large organic falls to the benthic environment, such as dead wood or whale bones, harbour organisms relying on sulfide-oxidizing symbionts. Nothing is known however, concerning sulfide enrichment at the wood surface and its relation to wood colonization by sulfide-oxidizing symbiotic organisms. In this study we combined in situ hydrogen sulfide and pH measurements on sunken wood, with associated fauna microscopy analyses in a tropical mangrove swamp. This shallow environment is known to harbour thiotrophic symbioses and is also abundantly supplied with sunken wood. A significant sulfide enrichment at the wood surface was revealed. A 72 h sequence of measurements emphasized the wide fluctuation of sulfide levels (0.1->100 lM) over time with both a tidal influence and rapid fluctuations. Protozoans observed on the wood surface were similar to Zoothamnium niveum and to vorticellids. Our SEM observations revealed their association with ectosymbiotic bacteria, which are likely to be sulfide- oxidizers. These results support the idea that sunken wood surfaces constitute an environment suitable for sulfide-oxidizing symbioses.
ISSN: 0141-1136

hal-00751649
https://hal.univ-antilles.fr/hal-00751649

Éditeur(s) : HAL CCSD Station Biologique
Résumé : International audience
Four "morphotypes" of Mytilidae were collected from wood falls in the west Pacific (Vanuatu islands) and in the Bohol sea (Philippines) between 300 and 1800 metre depth. Results from our study demonstrated the existence of extracellular bacteria located between microvilli at the apical surface of the cells all along the lateral zone of the gill filaments in the four mytilid morphotypes analyzed. Based on TEM observations, these Gram-negative bacteria were not methanotrophic due to the lack of concentric stacking of intracellular membranes in their cytoplasm. Based on FISH experiments, these bacteria belong to g-Proteobacteria. These preliminary observations described gill symbiosis-like interactions in metazoan species associated with sunken wood, strengthening the hypothesis that decomposing wood may serve as steps for the introduction of symbiotic Mytilidae to vents and seeps. Phylogenetic analysis of these extracellular bacteria are in progress in our lab in order to compare their 16SrDNA sequences to that of other marine invertebrate symbionts described to date.
ISSN: 0007-9723

hal-00753023
https://hal.univ-antilles.fr/hal-00753023

Éditeur(s) : HAL CCSD Elsevier Masson
Résumé : International audience
Mussels of the subfamily Bathymodiolinae thrive around chimneys emitting hot fluids at deep sea hydrothermal vents, as well as at cold seeps and on sunken organic debris (sunken wood, whale falls). Despite the absence of light-driven primary production in these deep-sea ecosystems, mussels succeed reaching high biomasses in these harsh conditions thanks to chemosynthetic, carbon-fixing bacterial symbionts located in their gill tissue. Since the discovery of mussel symbioses about three decades ago our knowledge has increased, yet new findings are published regularly regarding their diversity, role and evolution. This article attempts to summarize current knowledge about symbiosis in Bathymodiolinae, focusing on mussel species for which information is available regarding both hosts and symbionts. Moreover, new data obtained from small mussels inhabiting sunken woods around the Philippines are provided. Indeed, mussel species from organic falls remain poorly studied compared to their vent and seep relatives despite their importance for the understanding of the evolution of symbiosis in the subfamily Bathymodiolinae.
ISSN: 1631-0691

hal-00750422
https://hal.univ-antilles.fr/hal-00750422

Éditeur(s) : HAL CCSD
Résumé :   Diffusion du document : INRA Antilles-Guyane, UMR ECOFOG, 97387 Kourou, Guyane (France) Diplôme : Master Recherche
il s'agit d'un type de produit dont les métadonnées ne correspondent pas aux métadonnées attendues dans les autres types de produit : DISSERTATION
The gravitropic reaction is a fundamental phenomenon in the life of the young trees in under-storey of a tropical rain forest. It enables them to preserve a growth in height even after a fall of remains or a wind fallen wood had leaning them. This reaction passes mainly by the differentiation of reaction wood and the offsetting of the radial growth. The gravitropic reaction is also under the control of various parameters such as the diameter of the stems, the hardness of wood, or masses they carried. The tropical rain forest contains a great specific diversity and ecological strategies. In this work we seek to know if this diversity is reflected in the strategies of recovery of the species in particular on a level of efficiency of reaction. We compared eight species of tropical rain forest with various ecological behaviours. We studied their changes in form in the course of time, as to their growth. We showed that there are indeed interspecific differences in the gravitropic reaction. Some behaviours are specific to some species. We also showed that for the majority of the species, the deformation energy is strongly correlated with the relative growth of the species.
Le redressement gravitropique est un phénomène fondamental dans la vie des jeunes arbres en sous-bois de forêt tropicale. Il leur permet de conserver une croissance en hauteur même après avoir subi la chute de débris ou même un chablis ayant conduit à leur inclinaison. Ce redressement passe principalement par la différentiation de bois de réaction et l’excentrement de la croissance radiale. Le redressement gravitropique est également sous le contrôle de différents paramètres tels que le diamètre des tiges, la dureté du bois, ou les masse portées. La forêt tropicale humide contient une grande diversité spécifique et de stratégies écologiques. Dans ce travail nous cherchons à savoir si cette diversité se reflète dans les stratégies de redressement des espèces notamment au niveau de l’efficience de la réaction. Nous avons comparé huit espèces de forêt tropicale humide avec différents comportements écologiques. Nous avons étudié leurs changements de forme au cours du temps, ainsi que leur croissance. Nous avons réussi à montrer qu’il existe effectivement des différences interspécifiques de redressement. Des comportements particuliers à certaines espèces ont pu être dégagés. Nous avons également montré que pour la majorité des espèces, l’énergie de déformation est fortement corrélée à la croissance relative de l’espèce.
https://hal.archives-ouvertes.fr/hal-01189172

hal-01189172
https://hal.archives-ouvertes.fr/hal-01189172
PRODINRA : 11396

Éditeur(s) : HAL CCSD Wiley-Blackwell
Résumé : International audience
Six morphotypes of small mussels (Bivalvia: Mytilidae) were found attached to naturally sunken wood collected in the Bohol Sea (Philippines). These specimens are related to the large Bathymodiolus mussels that are found worldwide at cold seeps and hydrothermal vents. In these habitats, the mytilids harbour sulphur- and methane-oxidizing endosymbionts in their gills and depend on the energy and carbon provided by the symbionts. In this study, bacteria associated with the gills of wood-associated mussels are characterized using molecular and microscopic techniques. The existence of bacteria in the lateral zone of gill filaments in all specimens is demonstrated. Comparative analyses of 16S rRNA gene and adenosine 5′-phosphosulphate (APS) reductase gene sequences indicate that the bacteria are closely related to sulphur-oxidizing endosymbionts of Bathymodiolus. FISHs using specific probes confirm that sulphur oxidizers are by far the most abundant, if not the only bacteria present. Electron micrographs displayed mostly extracellular bacteria located between microvilli at the apical surface of host gill epithelial cells all along the lateral zone of each gill filament. In some specimens, occasional occurrence of intracellular bacteria with similar morphology was noted. This study provides the first molecular evidence for the presence of possible thiotrophic symbiosis in sunken wood ecosystems. With their epibiotic bacteria, wood-associated mussels display a less integrated type of interaction than described in their seep, vent and whale fall relatives.
ISSN: 0168-6496

hal-00715244
https://hal.univ-antilles.fr/hal-00715244
DOI : 10.1111/j.1574-6941.2008.00438.x

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Ciliates represent a diversified group of protists known to establish symbioses with prokaryotic microorganisms. They are mainly phagotrophs and symbiotic relationships with bacteria can give them an important advantage in chemosynthetic environments. The aim of this study is to describe the thiotrophic association that occurs between the peritrich ciliate Pseudovorticella sp. and potential sulfur-oxidizing bacteria. Investigations at microscopic scale (LM, SEM, TEM) showed ectosymbiotic bacteria covering the surface of the body of Pseudovorticella sp. According to 16S rDNA phylogenetic analysis, these ectosymbiotic bacteria belong to-proteobacteria and are phylogenetically close to the symbiont of the recently described Zoothamnium ignavum, which inhabits shallow-water wood falls. FISH experiments, using symbiont specific probes, clearly indicate that these ectosymbiotic bacteria are also ingested into food vacuoles. Electron lucent granules observed in TEM in the cytoplasm of the ectosymbiotic bacteria have been identified as sulfur granules by Raman microspectrometry analyses. Raman microspec-trometry analyses confirmed the thiotrophic nature of this relationship already suggested by the results obtained by TEM and phylogeny. A complete sulfur map was then performed to investigate the sulfur distribution in the zooid. Results show that the relationship between this protist and its bacterial partner is a thiotrophic ectosymbiosis.
ISSN: 0932-4739

hal-01655933
http://hal.upmc.fr/hal-01655933
http://hal.upmc.fr/hal-01655933/document
http://hal.upmc.fr/hal-01655933/file/GRIMONPREZ_Adrien_sans%20marque.pdf
DOI : 10.1016/j.ejop.2017.11.002

Éditeur(s) : HAL CCSD Springer Verlag
Résumé : International audience
ISSN: 0720-213X

hal-00755290
https://hal.univ-antilles.fr/hal-00755290
DOI : 10.1007/s00435-007-0035-3