Manioc : Portail | resultats 1 - 19 pour la recherche: Aguirre Guzman, G

Histoire généralle des Indes occidentales, et Terres-Neuves qui jusques à présent ont esté descouvertes, augmentee en ceste cinquiesme edition de la description de la nouvelle Espagne, & de la grande ville de Mexicque, autrement nommee, Tenuctilan: composee en espagnol par François Lopez de Gomara, & traduite en françois par le S. de Genillé Mart. Fumée
Auteur(s) : López de Gómara, Francisco (1511-1566)
Auteurs secondaires : Fumée Genillé, Martin (1540-1590)
Année de publication :

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Voyage pittoresque dans les deux Amériques : résumé général de tous les voyages
Auteur(s) : Orbigny, Alcide d' (1802-1857)
Auteurs secondaires : Sainson
Année de publication :

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Anecdotes américaines ou Histoire abrégée des principaux événements arrivés dans le nouveau monde, depuis sa découverte jusqu'à l'époque présente
Auteur(s) : Hornot, Antoine (17..-....)
Année de publication :

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Cuba por fuera (apuntes del natural)
Auteur(s) : Gallego Garcia, Tesifonte
Année de publication :

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Proteinaceous exotoxins of shrimp-pathogenic isolates of Vibrio penaeicida and Vibrio nigripulchritudo
Auteur(s) : Aguirre-guzman, Gabriel Labreuche, Yannick Ansquer, Dominique Espiau, Benoit Levy, Peva Ascencio, Felipe Saulnier, Denis

Éditeur(s) : Universidad Autonoma de Baja California
Résumé : The pathogenicity of two V. penaeicida strains, AM101 and KH-1, with different geographic origin, and V. nigripulchritudo strain AM102, were investigated in juvenile blue shrimp species Litopenaeus stylirostris. Alive bacteria and protein fractions (PFs) obtained from cell-free supernatants (CFS) were used in experimental challenges. Strains AM102, AM101, and KH-1 produced respectively 60, 54 and 12% mortality at 96h after infection using 104 cfu mL-1 of bacterial suspension. Exocellular toxin-like factors were evidenced in CFS from the New Caledonian strains (AM102 and AM101) but not the Japanese strain (KH-1). At 48h post injection of each CFS, mortality rates were respectively 96,98 and 5% when these strains were cultivated at 20°C, whereas only O,16 and 5% mortality rates were observed when these strains were cultivated at 30°C. Clear differences in pathogenicity between both V. penaeicida strains of distinct geographic origin (AM101 and KH-1) were thus emphasized. Protein fractions were obtained from CFS of all the strains tested in this study and cultivated at 20°C, by ammonium sulphate precipitation. Whatever the used strain, significantly higher mortalities were produced with PFs obtained with 0-40% of ammonium sulphate saturation, in respect to those produced with PFs60 and PFs80. Shrimp injected with PFs40 from strains AM102, AM101 and KH-1, and at a 20-µg org-1 dose produced respectively 100, 90 and 60% mortality 46h after the challenge. The strain AM101 showed a mediam lethal dose of approximately 5µg protein org-1 (1-1.25 µg protein g-1 body weight) 59h after injection. The PFs40 from New Caledonian strains were fond sensitive to heating and proteinase K treatments, reinforcing thus the hypothesis of their proteinaceous nature. Surprisingly, PFs40 from each bacterial strain displayed similar protein bands by SDS-PAGE suggesting that the tested strains share a common exotoxic compound regardless their distinct geographic origin or species.
Ciencias Marinas (Universidad Autonoma de Baja California), 2003 , Vol. 29 , N. 1 , P. 77-88

Droits : Universidad Autonoma de Baja California
http://archimer.ifremer.fr/doc/2003/publication-2807.pdf
http://archimer.ifremer.fr/doc/00000/2807/

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