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Antiplasmodial activities of dyes against Plasmodium falciparum asexual and sexual stages: Contrasted uptakes of triarylmethanes Brilliant green, Green S (E142), and Patent Blue V (E131) by erythrocytes
Auteur(s) : Leba, Louis-Jérôme Popovici, Jean Estevez, Yannick Pelleau, Stéphane Legrand, Eric Musset, Lise DUPLAIS, Christophe
Auteurs secondaires : Laboratoire de Parasitologie, Centre National de Référence du Paludisme - Région Antilles-Guyane ; Institut Pasteur de la Guyane - Réseau International des Instituts Pasteur (RIIP) - WHO Collaborating Center for Surveillance of Antimalarial Drug Resistance Université de Guyane (UG) Institut Pasteur du Cambodge Ecologie des forêts de Guyane (ECOFOG) ; Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD) - Institut National de la Recherche Agronomique (INRA) - Université des Antilles et de la Guyane (UAG) - AgroParisTech - Université de Guyane (UG) - Centre National de la Recherche Scientifique (CNRS) Génétique et Génomique des Insectes vecteurs ; Institut Pasteur [Paris] - Centre National de la Recherche Scientifique (CNRS) This work was supported by European commission (REGPOT-CT-2011-285837-STRonGer), by Institut Pasteur (ACIP A10-2013) and by Investissement d’Avenir of the ANR (CEBA: ANR-10-LABX-25-01) We thank MR4 for providing us Plasmodium falciparum 7G8 (MRA-926 contributed by Thomas Wellems) and 3D7 (MRA-102 contributed by Daniel J. Carucci) malaria parasites. ANR-10-LABX-25-01/10-LABX-0025, CEBA, CEnter of the study of Biodiversity in Amazonia(2010)

Éditeur(s) : HAL CCSD
Résumé : International audience
The search for safe antimalarial compounds acting against asexual symptom-responsible stages and sexual transmission-responsible forms of Plasmodium species is one of the major challenges in malaria elimination programs. So far, among current drugs approved for human use, only primaquine has transmission-blocking activity. The discovery of small molecules targeting different Plasmodium falcip-arum life stages remains a priority in antimalarial drug research. In this context, several independent studies have recently reported antiplasmodial and transmission-blocking activities of commonly used stains, dyes and fluorescent probes against P. falciparum including chloroquine-resistant isolates. Herein we have studied the antimalarial activities of dyes with different scaffold and we report that the triar-ylmethane dye (TRAM) Brilliant green inhibits the growth of asexual stages (IC 50 2 mM) and has exflagellation-blocking activity (IC 50 800 nM) against P. falciparum reference strains (3D7, 7G8) and chloroquine-resistant clinical isolate (Q206). In a second step we have investigated the antiplasmodial activities of two polysulfonated triarylmethane food dyes. Green S (E142) is weakly active against P. falciparum asexual stage (IC 50 x 17 mM) whereas Patent Blue V (E131) is inactive in both antimalarial assays. By applying liquid chromatography techniques for the culture supernatant analysis after cell washings and lysis, we report the detection of Brilliant green in erythrocytes, the selective uptake of Green S (E142) by infected erythrocytes, whereas Patent Blue V (E131) could not be detected within non-infected and 3D7-infected erythrocytes. Overall, our results suggest that two polysulfonated food dyes might display different affinity with transporters or channels on infected RBC membrane.
International journal for parasitology. Drugs and drug resistance

Droits : http://creativecommons.org/licenses/by-nc-sa/
pasteur-01614675
https://hal-pasteur.archives-ouvertes.fr/pasteur-01614675
https://hal-pasteur.archives-ouvertes.fr/pasteur-01614675/document
https://hal-pasteur.archives-ouvertes.fr/pasteur-01614675/file/Leba%20LJ-article%20colorant-version%20publiee.pdf
DOI : 10.1016/j.ijpddr.2017.07.002

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