Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : HAL CCSD Centre National de la Recherche Scientifique
Résumé : Une étude stratigraphique, sédimentologique et magnétique a été réalisée sur la partie supérieure d'une terrasse alluviale de l'oued Adaila, dansla plaine d'El Ma Labiod (nord-est de l'Algérie). Cette terrasse renferme un niveau très riche en matériel archéologique appartenant à la civilisation atérienne, dont l'âge dépasse 35 000 ans. Du point de vue sédimentologique, les sédiments sont dominés par la fraction limoneuse suivie par les sables fins et grossiers. Du point de vue stratigraphique, la succession de dépôts à éléments grossiers recouverts par des éléments fins correspond à des niveaux fluviatiles avec des mises en place à débit contrasté. Les courbes cumulatives ont un faciès dit " parabolique " et témoignent d'un sédiment immature et peu évolué. Les résultats de l'étude magnétique concordent avec les précédents. Ils révèlent une forte concentration en grains magnétiques dans la partie médiane de la coupe (foyer) et une faible concentration à la base (Unité L) contemporaine d'un climat sec. Les grains magnétiques, de taille variable (polydomaine, monodomaine et superparamagnétique), sont portés par la magnétite et la maghémite de faible champ coercitif, ainsi que par la goethite et l'hématite de fort champ coercitif. Les courbes de la susceptibilité magnétique en fonction de la température et les courbes de la décomposition de l'ARI (spectres de coercivité) confirment ces résultats.
ISSN: 1142-2904

hal-00750185
https://hal.archives-ouvertes.fr/hal-00750185