Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490

Éditeur(s) : Gauthier-Villars
Résumé : The Middle America Trench off Guatemala was transected by 24-channel seismic-reflection surveys, seismic-refraction surveys, and drilling with the Glomar Challanger . The drilling was doneat three sites on the oceanic Cocos plate and four sites on the Caribbean plate. These plates converge at about 10 cm/yr as indicated by global plate reconstruction. On the oceanic Cocos plate a basal chalk sequence of lower and middle Miocene age is overlain by a thin interval of abyssal clay. In contrast is the Cretaceous to lower Miocene claystone sequence recovered only at a site 3 km landward of the trench axis where drilling penetrated the hemipelagic slope deposits. A large amount of sediment along with ocean crust has been subducted during the present (Miocene to Quaternary) episode of subduction, and parts of the continental framework may have been subducted as well. No current tectonic model satisfactorily explains the surprising occurrence of Cretaceous to Miocene claystone at the foot of the continent.
Oceanologica Acta, Special issue (0399-1784) (Gauthier-Villars), 1981

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/00245/35671/34179.pdf
http://archimer.ifremer.fr/doc/00245/35671/

Éditeur(s) : HAL CCSD Wiley/Blackwell
Résumé : 4 pages
International audience
This article documents the addition of 268 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Alburnoides bipunctatus, Chamaerops humilis, Chlidonias hybrida, Cyperus papyrus, Fusarium graminearum, Loxigilla barbadensis, Macrobrachium rosenbergii, Odontesthes bonariensis, Pelteobagrus vachelli, Posidonia oceanica, Potamotrygon motoro, Rhamdia quelen, Sarotherodon melanotheron heudelotii, Sibiraea angustata, Takifugu rubripes, Tarentola mauritanica, Trimmatostroma sp. and Wallago attu. These loci were cross-tested on the following species: Alburnoides fasciatus, Alburnoides kubanicus, Alburnoides maculatus, Alburnoides ohridanus, Alburnoides prespensis, Alburnoides rossicus, Alburnoides strymonicus, Alburnoides thessalicus, Alburnoides tzanevi, Carassius carassius, Fusarium asiaticum, Leucaspius delineatus, Loxigilla noctis dominica, Pelecus cultratus, Phoenix canariensis, Potamotrygon falkneri, Trachycarpus fortune and Vimba vimba.
ISSN: 1755-098X

hal-00823716
https://hal.archives-ouvertes.fr/hal-00823716
DOI : 10.1111/1755-0998.12095
PUBMED : 23521844

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
The Paleozoic-early Mesozoic geology of Tibet was controlled by the rift-drift, seafloor spreading and subduction zone tectonics of a Paleo-Tethyan realm, which evolved between the West Cathaysides (WC) and the East Cimmerides (EC). Different suture zones with ophiolites and ophiolitic mélanges, high-pressure metamorphic belts, magmatic arcs and accretionary prism complexes separating different terranes mark multiple subduction-accretion systems within this Paleo-Tethyan domain, reminiscent of the modern Western Pacific Ocean. Discrete basins separated by different continental blocks and magmatic arcs constituted a complex paleogeography of Paleo-Tethys, and these oceanic strands were closed as a result of subduction with different polarities during the late Paleozoic-Triassic. The Longmu Tso Shuanghu-Changning Menglian Suture zone (LS-CMS) in Tibet represents the main tectonic boundary between the WC and EC that developed in the Devonian. The East Kunlun-A’nyemaqen oceanic slab was subducted northward beneath the East Kunlun terrane in northern Tibet, whereas the Sumdo oceanic slab was subducted northward beneath the South Qiangtang-North Lhasa terrane in southern Tibet. The Longmu Tso Shuanghu-Changning Menglian and Jinshajiang-Ailaoshan-Song Ma ophiolites were developed and emplaced in subduction-accretion systems with opposite polarities (westward and eastward) beneath the North Qiangtang-Qamdo-Simao-Indochina terrane in central Tibet. The Jinshajiang-Ailaoshan-Song Ma oceanic slab was subducted westward beneath the North Qiangtang-Simao-Indochina terrane along the Jinshajiang and Ailaoshan-Song Ma trenches in a trench-ridge-trench triple junction plate configuration. The Emeishan mantle plume produced a large Permian basaltic terrane, developed on the western passive margin of the South China block. The final closure of the Paleo-Tethyan oceanic branches resulted in continental collisions and development of a vast Indosinian orogenic collage in the latest Triassic-Jurassic.
ISSN: 0743-9547

insu-01119604
https://hal-insu.archives-ouvertes.fr/insu-01119604
https://hal-insu.archives-ouvertes.fr/insu-01119604/document
https://hal-insu.archives-ouvertes.fr/insu-01119604/file/Xu-JAES-2015.pdf
DOI : 10.1016/j.jseaes.2015.01.021