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Defining the human sperm microtubulome: an integrated genomics approach
Auteur(s) : Jumeau, Fanny Chalmel, Frédéric Fernandez-Gomez, Francisco-Jose Carpentier, Celine Obriot, Hélène TARDIVEL, Meryem Caillet-Boudin, Marie-Laure Rigot, Jean-Marc
Auteurs secondaires : Centre de recherche Jean-Pierre Aubert-Neurosciences et Cancer ; Institut National de la Santé et de la Recherche Médicale (INSERM) - Université de Lille, Droit et Santé Institut de recherche, santé, environnement et travail [Rennes] (Irset) ; Université d'Angers (UA) - Université des Antilles et de la Guyane (UAG) - Université de Rennes 1 (UR1) - École des Hautes Études en Santé Publique [EHESP] (EHESP) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ) Alzheimer & Tauopathies (Equipe 1) ; Université de Lille - Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille) - Centre de Recherche Jean-Pierre AUBERT - Neurosciences et Cancer (JPArc) ; Institut National de la Santé et de la Recherche Médicale (INSERM) - Université de Lille, Droit et Santé - Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille) - Institut National de la Santé et de la Recherche Médicale (INSERM) - Université de Lille, Droit et Santé Université Lille 2 - Faculté de Médecine Institut de médecine predictive et de recherche thérapeutique (IMPRT) ; Institut Pasteur de Lille - CRLCC Oscar Lambret - Institut National de la Santé et de la Recherche Médicale (INSERM) - Université de Lille, Droit et Santé - Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille) Université de Rouen Normandie (URN) ; Normandie Université (NU) Lille University Medical Center (CHRU de Lille) INSERM

Éditeur(s) : HAL CCSD Society for the Study of Reproduction
Résumé : International audience
Spermmotility notably depends on the structural integrity of the flagellum and the regulation ofmicrotubule dynamics. Although researchers have started to use " omics" techniques to characterize the human sperm's molecular landscape, the constituents responsible for the assembly, organization, and dynamics of the flagellum microtubule have yet to be fully defined. In this study, we defined a core set of 116 gene products associated with the human sperm microtubulome (including products potentially involved in abnormal ciliary phenotypes and male infertility disorders). To this end, we designed and applied an integrated genomics workflow and combined relevant proteomics, transcriptomics, and interactomics datasets to reconstruct a dynamic interactome map. By further integrating phenotypic information, we defined a disease-interaction network; this enabled us to highlight a number of novel factors potentially associated with altered sperm motility and male fertility. Lastly, we experimentally validated the expression pattern of two candidate genes (CUL3 and DCDC2C) that had never previously been associated with male germline differentiation. Our analysis suggested that CUL3 and DCDC2C's products have important roles in the sperm flagellum. Taken as a whole, our results demonstrate that an integrated genomics strategy can highlight relevant molecular factors in specific sperm components. This approach could be easily extended by including other " omics" data (from asthenozoospermic men, for example) and identifying other critical proteins from the human sperm microtubulome. Summary Sentence Although researchers have started to use "omics" techniques to characterize the human sperm's molecular landscape, the constituents responsible for the assembly, organization, and dynamics of the flagellum microtubule have yet to be fully defined.
ISSN: 0006-3363

hal-01515563
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01515563
DOI : 10.1095/biolreprod.116.143479
PUBMED : 28395323

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