Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
Selective serotonin reuptake inhibitor medication exposure during the perinatal period can have a long term impact in adult offspring on neuroplasticity and the serotonergic system, but the impact of these medications during early development is poorly understood. The aim of this study was to determine the effects of developmental exposure to the SSRI, fluoxetine, on the serotonergic system, dopaminergic system, and synaptophysin density in the prefrontal cortex and hippocampus, as well as number of immature neurons in the dentate gyrus, in juvenile rat offspring at weaning. To model aspects of maternal depression, prenatal restraint stress was used. Sprague-Dawley rat offspring were exposed to either prenatal stress and/or fluoxetine. Main findings show that developmental fluoxetine exposure to prenatally stressed offspring decreased 5-HT and 5-HIAA levels and altered the dopaminergic system in the hippocampus. Prenatal stress, regardless of fluoxetine, increased synaptophysin density in the PFC. This work indicates that early exposure to maternal stress and SSRI medication can alter brain monoamine levels and synaptophysin density in offspring at weaning. © 2015 Wiley Periodicals, Inc. Dev Psychobiol
ISSN: 0012-1630

hal-01237091
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01237091
DOI : 10.1002/dev.21372
PUBMED : 26477449

Résumé : Different seabass (Dicentrarchus labrax) intensiv rearing technics have been experimented in the pilot scale unit of our laboratory. During these upscalfing operation, the results obtained allow us to compare costs related to each fingemng production method. Closed system combined with early weaning with an efficient micropellet saves at least 69 % of direct production costs by comparison with an open system with Arternia during larval phase. However if closed systems are commonly used by private hatcheries, larval micropellets are always at experimental step.
Cet article fait le point sue les coûts de production directs relatifs à différentes méthodes d'élevage intensif du loup (Dicentrarchus labrax). Il s'appuie sur des résultats zootechniques obtenus dans l'unité de démonstration à grande échelle de notre laboratoire. Par rapport à un système de production en circuit ouvert, le recyclage de l'eau permet de diviser par 2.2 les charges directes de production. Si les proies vivantes sont précédemment remplacées par une microparticule efficace, ces charges sont alors divisées par 3.2. Les techniques de recyclage de l'eau ont franchi les portes du laboratoire pour être couramment utilisées par les professionnels, alors que les aliments articifiels convenant au stade larvaire sont toujours au stade expérimental.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/1993/rapport-1916.pdf
http://archimer.ifremer.fr/doc/00000/1916/

Éditeur(s) : Elsevier Science Bv
Résumé : In this study, three microdiets were tested on weaning of Florida pompano larvae: Otohime, Gemma and a reference diet LR803. The experimental system was stocked with 11-day-old larvae, which were co-fed micro-diets and live food from 11 dph to 17 dph then micro-diets only until 28 dph. Survival from 11 dph to 28 dph was similar for all treatments, with an average of 33%. At the end of the trial, the Gemma larvae were significantly longer and heavier than larvae fed the other diets. Significant differences were observed in fatty acid composition of the diets and larvae between treatments. The Gemma larvae incorporated the lowest amount of eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and arachidonic acid (ARA). However, they had the highest DHA/EPA and ARA/EPA ratios, which is in agreement with the concept that the proportions of polyunsaturated fatty acids could be of greater importance than their absolute amount. Results from the enzyme analysis suggest that fishmeal is suitable as the main protein source for Florida pompano larvae compared to krill meal. This study gives new insights on Florida pompano early nutritional requirements and demonstrated the full functionality of the pancreas at 16 days post hatch, opening possibilities of an earlier weaning time.
Aquaculture (0044-8486) (Elsevier Science Bv), 2014-02 , Vol. 422 , P. 268-276

Droits : 2013 Elsevier B.V. All rights reserved
http://archimer.ifremer.fr/doc/00169/28067/26282.pdf
DOI:10.1016/j.aquaculture.2013.12.006
http://archimer.ifremer.fr/doc/00169/28067/

Éditeur(s) : HAL CCSD Wiley
Résumé : International audience
With the growing use of selective serotonin reuptake inhibitor medications (SSRIs) for the treatment of depression during the perinatal period, questions have been raised about the longterm impact of these medications on development. We aimed to investigate how developmental SSRI exposure may alter affect-related behaviors and associated molecular processes in offspring using a rodent model of maternal stress and depression. For this purpose, prenatally stressed or non-stressed male offspring were exposed to fluoxetine (5 mg/kg/day) or vehicle, via lactation, until weaning. Primary results show that postnatal fluoxetine exposure differentially altered anxiety-like behavior by increasing anxiety in non-stressed offspring and decreasing anxiety in prenatally stressed offspring. In the hippocampus, developmental fluoxetine exposure decreased BDNF IV and TrkB mRNA expression. Prenatal stress alone also decreased escape behaviors and decreased hippocampal BDNF IV mRNA expression. These data provide important evidence for the long-term programming effects of early-life exposure to SSRIs on brain and behavior.
ISSN: 0012-1630

hal-01305478
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01305478
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01305478/document
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01305478/file/Developmental%20SSRIs%2C%20anxiety%20and%20BDNF_accepted.pdf
DOI : 10.1002/dev.21385

Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
Subcutaneous fat tissues from an indigenous fat-type breed and an intensively-lean selected breed were studied in juvenile pigs. Combining DIGE with bioinformatics and target analyses of key genes, enzymes or terminal routes, this study identifies metabolic and homeostatic processes, response to organic substances, and acute-phase responses as the main pathways whose proteins were regulated in association with adiposity. Breed-related differences in abundance and activities of malic enzyme and glucose-6-phosphate dehydrogenase NADPH-supplying enzymes suggested up-regulation of the lipogenic pathway to dispose for a greater adiposity. Over-abundance in the lipolytic protein carboxylesterase-1 was revealed in fat-type piglets. A panel of pro- and anti-inflammatory proteins such as serpins, had an altered abundance in the fat-type piglets, suggesting adverse consequences of fat accumulation even in early post-weaning stages. Propensity to low-grade inflammation in fat pigs was reinforced by the up-regulation of genes encoding pro-inflammatory cytokines IL6 and TNF-α in these piglets. Differential abundance in annexin-A5 and pericentrin suggested a positive regulation of cell apoptosis in lean piglets. Our results are relevant in the context of data linking the accretion of body lipids to the physiology and pathology of adipose tissue in models other than rodents for a better control of human health and nutrition.
ISSN: 1874-3919

hal-01122161
https://hal-univ-rennes1.archives-ouvertes.fr/hal-01122161
PUBMED : 22061664
DOI : 10.1016/j.jprot.2011.10.012

Éditeur(s) : Proceedings of the 59th Annual Gulf and Caribbean Fisheries Institute
Résumé : In most of marine fishes cultured, larvae in their early development are fed with live prey (rotifer and/or artemia). In hatcheries, culture of these preys is labour consuming, needs specific facilities and represent an important part of production cast. On the other hand, world artemia production is subjected to fluctuation of harvest in the wild which could Iimit fish farming industry in the near future. A dried formula adapted to marine fish larvae has been perfected by Ifremer's center of Brest (France) and is distributed by a feeding company since 1999. This artificial microbound diet has been successfully tested on several temperate fishes such as European seabass (Dicentrarchus labrax), Atlantic cod (Gadus morhua) or European seabream (Sparus aurata) in a co-feeding (i.e. artemia and dried diet) strategy. Experiments described here were carried out ta evaluate the performances obtained during total substitution of artemia by this diet in Red drum (Sciaenops ocellatus) larval culture. ln 2004 and 2005, four experiments were conducted on feed technology aspects (experimental or industrial manufacturing), weaning strategy (direct or co-feeding) and feed distribution method (prototypes or classical self feeder) in comparison to the standardized method using live preys. At the end of larval phase (day 15) and at day 30, end of nursery phase (0.6 g), survival relative to control (RTC) was respectively 96.9% and 111.0% in the best treatment. Concerning growth, the weight RTC reached at day 15 and day 30 was respectively 82.8% and 72.4%. However, it is important to note that rearing protocols have to be adapted ta the use of these micro pellets in order toprevent degradation of culture quality. These results show that this new product can be applied on industrial scale in private hatcheries. But further research is needed ta evaluate replacement of rotifers by this dried formula in order to completely eliminate live prey in larval culture of Red drum.
Dans la plupart des poisons marins élevés, les larves sont nourries avec des proies vivantes (rotifères et/ou artémia) au début de leur développement. Dans les écloseries, la culture de ces proies est consommatrice de main d'oeuvre, nécessite des installations spécifiques et entre pour une part importante dans le coût de production. Par ailleurs, la production mondiale d'artémia est dépendante de la fluctuation des récoltes dans le milieu naturel, ce qui pourrait gêner la filière piscicole marine dans un futur proche. Une formulation alimentaire inerte adaptée aux larves de poisson marin a été mise au point par le centre Ifremer de Brest (France) et est commercialisée par un provendier depuis 1999. Ces microparticules artificielles ont été testée avec succès sur plusieurs poissons tempérés comme le bar européen (Dicentrarchus labrax), la morue atlantique (Gadus morha) ou la daurade européenne (Sparus aurata) dans un contexte de co-feeding (i.e. artémia et aliment inerte). Les expériences décrites ici ont été menées afin d'évaluer les performances obtenues lors de la substitution totale des artémia par cet aliment dans l'élevage larvaire de l'ombrine (Sciaenops ocellatus). En 2004 et 2005, quatre expériences ont été menées sur l'aspect technologie alimentaire (fabrication expérimentale ou industrielle), la stratégie de sevrage (direct ou co-feeding) et le mode de distribution de l'aliment (distributeur classique ou prototypes) en comparaison au standard sur proies vivantes. En fin de phase larvaire (jour 15) et au jour 30 après une phase de prégrossissement (0.6g), la survie par rapport au témoin (RTC) était respectivement de 96.9% et 111 .1% dans le meilleur traitement. Concernant la croissance, les poids obtenus aux jours 15 et 30 atteignaient respectivement 82.8% et 72.4%. Cependant il est important de relever que les protocoles d'élevage doivent être adaptés à l'utilisation de ces microparticules afin de prévenir une dégradation de la qualité d'élevage. Ces résultats montrent que ce nouveau produit peut être appliqué à l'échelle industrielle dans les écloseries privées. Cependant, les recherches doivent être poursuivies pour évaluer les possibilités de remplacement des rotifères par ces aliments inertes afin de s'affranchir complètement de l'utilisation de proies vivantes dans l'élevage larvaire de l'ombrine.

Droits : info:eu-repo/semantics/openAccess
http://archimer.ifremer.fr/doc/2006/acte-6597.pdf
http://archimer.ifremer.fr/doc/00000/6597/