Éditeur(s) : HAL CCSD Elsevier
Résumé : International audience
We present new, full geomagnetic field vector results from three Neolithic ovens discovered at the archaeological site of Portonovo (Marche, Italy). The discovered structures are a rare example of very well preserved underground ovens from the Early Neolithic period. Standard thermal demagnetization procedures were used to isolate the direction of the Characteristic Remanent Magnetization acquired by the baked clay during the ovens' last firing. The corresponding archaeointensities were determined by the multi-specimen procedure (MSP-DSC) and show a clear intensity low during the Neolithic period. Both directional and intensity results are of high quality, offering the first contribution of full geomagnetic field vector data for this period in Italy. The new data are compared with other contemporaneous data from Europe and with global geomagnetic field models. Independent archaeomagnetic dating of the three ovens was also performed by means of the SCHA.DIF.14k model. The obtained results are in excellent agreement with available radiocarbon dates and confirm that all ovens belong to the Neolithic. These new data importantly enrich our knowledge of the geomagnetic field during the Neolithic period that is poorly documented by data, not only in Italy but also in the whole of Europe and show that archaeomagnetic dating can provide precise results even for prehistoric periods.
ISSN: 0012-821X

hal-01357276
https://hal.archives-ouvertes.fr/hal-01357276
DOI : 10.1016/j.epsl.2016.05.003

Éditeur(s) : HAL CCSD Springer Verlag (Germany)
Résumé : We present a detailed rock-magnetic and archaeomagnetic study of two brick kilns, named OSA and OSB, discovered at the location of Osterietta, in northern Italy. The magnetic properties of representative samples have been investigated to identify the nature of the magnetic carriers, their domain state and thermal stability, and investigate their suitability for archaeomagnetic determinations. Thermally stable, mainly pseudosingle domain (PSD) magnetite is identified as the main magnetic carrier. The full geomagnetic field vector has been determined for the two kilns, including directional and intensity analysis. Archaeointensities have been recovered with both the classical Thellier and the multi-specimen protocols. The multi-specimen procedure was performed with a very fast-heating oven developed at Montpellier, France. A Matlab® code for anisotropy correction during the Thellier experiment is provided. The archaeointensity results obtained from both techniques for the OSA kiln are of high quality and in good mutual agreement. For the OSB kiln, Thellier results are characterized by large standard deviation and the multi-specimen (MSP) technique was not successful. The obtained full geomagnetic field vector (declination, inclination and intensity) has been used for the archaeomagnetic dating of the two structures suggesting that the OSA kiln was for the last time used between 1761?1841 A.D. and the OSB kiln between 1752?1831 A.D., at 95% probability. This study shows that intensity determinations do not restrict the dating results when referring to the last few centuries, as this period is characterized by very small intensity variations.
ISSN: 0039-3169

hal-01236052
https://hal.archives-ouvertes.fr/hal-01236052
DOI : 10.1007/s11200-015-0413-0

Éditeur(s) : HAL CCSD Landes Bioscience
Résumé : International audience
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
Autophagy

hal-00735751
https://hal.archives-ouvertes.fr/hal-00735751
PUBMED : 22966490